UBE3B (NM_183415) Human 3' UTR Clone

CAT#: SC217113

3`UTR clone of ubiquitin protein ligase E3B (UBE3B) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE3B
• Synonyms: BPIDS; KOS
• ACCN: NM_183415
• Insert Size: 1917
• Sequence Data:
  Insert Sequence

  >SC217113 3'UTR clone of NM_183415
  The sequence shown below is from the reference sequence of NM_183415. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGGCTTTGAACTCTCCTAGCTCCTGTCCCAGCCCTGCCTCCAGGGCTCCTGGGCTGCCAGGGACCTTCAG
  CTCCCAGAGGCAGTGTGGTCCTGGGAATGTGACCAACATGCCAGGTGACATTGGCCCCTAGACCCTCTCT
  ATAGCCATGAGACTCCTTGTGGCCTCAAGAAATTTAGACGCCCACGACAGCACTACACAGCATCTCCAGG
  TGATGCCCAAGGCACAGGGCTGCAGAAAATAAACCTCCAGATTCCACCAACACGGGTCCATTCTTCCTGG
  TGATGGCAGAGGGGCTTCTTTTAGCTAGTTTGATCTTTTGGGAGTCTGTCTTTCCTTAGCCGTCTGAGTG
  AGCTGTGTATGAACAAGTCCCAGGAGTTCCAAGAGTCTAGAGTGGTTTTTGCAGCATGGGTTGAGTGTAC
  AAAGCCTACTGTGCGTGAGATCCTCTCCTTCCGTTTCTGAAATCTCTTACTCAGGTAAGGCCTCGCCAAG
  CCTCTATGCACCCCACAAAGTTTCTGCCTCCATGCCGTCCACAGCGCCTCTTCCCAGACAGCCAGGCCCA
  TCTGCTGCCCAGGGAAGCGCAGGCGCCTGCTAGGGACGCTATGGACACCGTGAGTCCAAGGCGCTGCTCC
  TGCCTTGAAGCCACGCGCTCCACGCCGCGGCCCTCCCATTTTCTGCGTCCTCAGCGGGCTGAGCTGCCAG
  AGAGTCTTCCCGGACCTATTCCCGTCCTATGCATTCACATTGGCATCCTGGTTTGGGGGAAGAAAAACAA
  CGGCCCTTAGCAGCAGCCCCGTTTCCAGAATGTGCTGCCTGTTCCCCAAAGCCTGCTTGTCCCGCGGAGG
  ACGGCTGCCTTTGACCCTGCTTATTTGTCTCACTGGTTATCTAATGAGGAACAAACACTAACCTAAGGTA
  CACATCCCATCTGGGCGGTGGCTTCACTCCTGAGTCTGCAAAATCCCAGGAAACTTGGGTCTTGCTGCCC
  ATCCTTCCTCAGCACAGGGAACCCGGAAGCCCGTTGCACTGACAGAGGCTCACACCCTCTGGGTTTTTTG
  TTTTTTTTTTAAACTTCATTTCTCTTCCAACCCATTGTGTTCCTCTGCCCTCATTCTTTACCTTTGTTGT
  AAGAACTTTAGCTCCAGGGAACTGAGGCAGAGGCCCTGTTTGTGGTCTGTGTTGACAGCCACCCACCCTC
  TCCCACCTCATGCTCCTGCACCTGCACCACCAAGGTTGATGCCGGATTCGAAGCCAGGAAGGCCCAGTCC
  CTCCTCTGCTCTCCTCAAAGACGCAAAACATTTTCCAACAAGAGCTGTCACAGTGGCAGCAAAGCAGGGG
  CCACCCGTCTCCACAGTCCTTCAGAGTTTCAGCTGCATCCTGGTGTCCCACCCAGGTCGAGCCCCCAGGC
  TTCTGGAAAAGAGTGTGTGCTCTACTTTGATGGAAACATGGCAAGGAATTTAAAGACAGGAATGTATTAA
  TATTATTGAAGGTGTGTTCGTAACCTCTGATTCTGTGGACTTGCCACTTTCTCCAAACGCTCGGTTCCTT
  TGAAGATTTCTTCTGAACGTGTGTGCGCACGCTGGGCGGGTTCGTGCATACATGCGGGGACCCCAGACTG
  TCAGCACAGGGAAGATGGATCCCATCCTAATTTTTATCACCTGAAGGTTGGAACCAGTGAGGGACTGGGA
  GAGAGTGATTTATAAGCACCAATATCAACTTCATGTGGATTTTTGACAAGGAGGGGTAGTTTGTAATTTC
  ATTTAAATTCTTTTCAGCAGCTGGAAATATTGCACTATCTGAAACACTGAATCTCCTTTTGTAACTGGTG
  TTCACTGACACCTTGATGGCTCTTGATGGCTCTAAAAAGTTGTAGGATTTTTTGTTTTTGTAGCTAACTT
  ATGGATTGAGATGTGATCAAAGGCTTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_183415.1
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]
• Locus ID: 89910