UBE3B (NM_183415) Human 3' UTR Clone
CAT#: SC217113
3`UTR clone of ubiquitin protein ligase E3B (UBE3B) transcript variant 3 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | UBE3B |
Synonyms | BPIDS; KOS |
ACCN | NM_183415 |
Insert Size | 1917 |
Sequence Data |
>SC217113 3'UTR clone of NM_183415
The sequence shown below is from the reference sequence of NM_183415. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC GGGCTTTGAACTCTCCTAGCTCCTGTCCCAGCCCTGCCTCCAGGGCTCCTGGGCTGCCAGGGACCTTCAG CTCCCAGAGGCAGTGTGGTCCTGGGAATGTGACCAACATGCCAGGTGACATTGGCCCCTAGACCCTCTCT ATAGCCATGAGACTCCTTGTGGCCTCAAGAAATTTAGACGCCCACGACAGCACTACACAGCATCTCCAGG TGATGCCCAAGGCACAGGGCTGCAGAAAATAAACCTCCAGATTCCACCAACACGGGTCCATTCTTCCTGG TGATGGCAGAGGGGCTTCTTTTAGCTAGTTTGATCTTTTGGGAGTCTGTCTTTCCTTAGCCGTCTGAGTG AGCTGTGTATGAACAAGTCCCAGGAGTTCCAAGAGTCTAGAGTGGTTTTTGCAGCATGGGTTGAGTGTAC AAAGCCTACTGTGCGTGAGATCCTCTCCTTCCGTTTCTGAAATCTCTTACTCAGGTAAGGCCTCGCCAAG CCTCTATGCACCCCACAAAGTTTCTGCCTCCATGCCGTCCACAGCGCCTCTTCCCAGACAGCCAGGCCCA TCTGCTGCCCAGGGAAGCGCAGGCGCCTGCTAGGGACGCTATGGACACCGTGAGTCCAAGGCGCTGCTCC TGCCTTGAAGCCACGCGCTCCACGCCGCGGCCCTCCCATTTTCTGCGTCCTCAGCGGGCTGAGCTGCCAG AGAGTCTTCCCGGACCTATTCCCGTCCTATGCATTCACATTGGCATCCTGGTTTGGGGGAAGAAAAACAA CGGCCCTTAGCAGCAGCCCCGTTTCCAGAATGTGCTGCCTGTTCCCCAAAGCCTGCTTGTCCCGCGGAGG ACGGCTGCCTTTGACCCTGCTTATTTGTCTCACTGGTTATCTAATGAGGAACAAACACTAACCTAAGGTA CACATCCCATCTGGGCGGTGGCTTCACTCCTGAGTCTGCAAAATCCCAGGAAACTTGGGTCTTGCTGCCC ATCCTTCCTCAGCACAGGGAACCCGGAAGCCCGTTGCACTGACAGAGGCTCACACCCTCTGGGTTTTTTG TTTTTTTTTTAAACTTCATTTCTCTTCCAACCCATTGTGTTCCTCTGCCCTCATTCTTTACCTTTGTTGT AAGAACTTTAGCTCCAGGGAACTGAGGCAGAGGCCCTGTTTGTGGTCTGTGTTGACAGCCACCCACCCTC TCCCACCTCATGCTCCTGCACCTGCACCACCAAGGTTGATGCCGGATTCGAAGCCAGGAAGGCCCAGTCC CTCCTCTGCTCTCCTCAAAGACGCAAAACATTTTCCAACAAGAGCTGTCACAGTGGCAGCAAAGCAGGGG CCACCCGTCTCCACAGTCCTTCAGAGTTTCAGCTGCATCCTGGTGTCCCACCCAGGTCGAGCCCCCAGGC TTCTGGAAAAGAGTGTGTGCTCTACTTTGATGGAAACATGGCAAGGAATTTAAAGACAGGAATGTATTAA TATTATTGAAGGTGTGTTCGTAACCTCTGATTCTGTGGACTTGCCACTTTCTCCAAACGCTCGGTTCCTT TGAAGATTTCTTCTGAACGTGTGTGCGCACGCTGGGCGGGTTCGTGCATACATGCGGGGACCCCAGACTG TCAGCACAGGGAAGATGGATCCCATCCTAATTTTTATCACCTGAAGGTTGGAACCAGTGAGGGACTGGGA GAGAGTGATTTATAAGCACCAATATCAACTTCATGTGGATTTTTGACAAGGAGGGGTAGTTTGTAATTTC ATTTAAATTCTTTTCAGCAGCTGGAAATATTGCACTATCTGAAACACTGAATCTCCTTTTGTAACTGGTG TTCACTGACACCTTGATGGCTCTTGATGGCTCTAAAAAGTTGTAGGATTTTTTGTTTTTGTAGCTAACTT ATGGATTGAGATGTGATCAAAGGCTTT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Reference Data | |
RefSeq | NM_183415.1 |
Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012] |
Locus ID | 89910 |
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