Lipoamide Dehydrogenase (DLD) (NM_000108) Human 3' UTR Clone

CAT#: SC217116

3`UTR clone of dihydrolipoamide dehydrogenase (DLD) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DLD
• Synonyms: DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3
• ACCN: NM_000108
• Insert Size: 1952 bp
• Sequence Data:
  Insert Sequence

  >SC217116 3'UTR clone of NM_000108
  The sequence shown below is from the reference sequence of NM_000108. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCTTGCTGCGTCATTTGGCAAATCAATCAACTTTTGAATTAGAAGATTATATATATTTTTTTCTGAAAT
  TTCCTGGGAGCTTTTGTAGAAGTCACATTCCTGAACAGGATATTCTCACAGCTCCAAGAATTTCTAGGAC
  TGAATTATGAAACTTTTGGAAGGTATTTAATAGGTTTGGACAAAATGGAATACTCTTATATCTATATTTT
  ACATAAATTTAGTATTTTGTTTCAGTGCACTAATGTGTAAGACAAAAAGCTACTTATTGTAGCATCCTGG
  AATATCTCCGTCAACTCATATTTTCATGCTGTTCATGAAAGATTCAATGCCCCTGAATTTAAATAGCTTT
  TTTCTCTGATACAGAAAAGTTGAATTTTACATGGCTGGAGCTAGAATTTGATATGTGAACAGTTGTGTTT
  GAAGCACAGTGATCAAGTTATTTTTAATTTGGTTTTCACATTGGAAACAAGTCAGTCATTCAGATATGAT
  TCAAATGTCTATAAACCGAACTGATGTAAGTAAACGGTCTCTCACTTGTTTTATTTAACCTCTAAATTCT
  TTCATTTTAGGGGTAGCATTTGTGTTGAAGAGGTTTTAAAGCTTCCATTGTTGTCTGCAACTCTGAAGGG
  TAATTATATAGTTACCCAAATTAAGAGAGTCTATTTACGGAACTCAAATACGTGGGCATTCAAATGTATT
  ACAGTGGGGAATGAAGATACTGAAATAAACGTCTTAAATATTCATTTACTGGTTATCATGAGTACGTGTT
  GAGATGGTCATAGTTTTTTTTATGACTACTTCTAGTGTATATTCTAATTTCTTTTCTAGGCCTGAATGTA
  TCTTTATTTTCATGTTATAGGACAATATTAAGGCATTTTAAAGGTCATCATCCTTTCATCTATTTTAGAT
  ACACCTACTAAATGTTTAATATATACTTTTGGAGAAGTACAACATAAGGGAGTCTTTAATCTGTGTTTTC
  CTTGGCTGGGTTAATGACTGTTTATTTAAAGAGTGTTGTAAAATTGGATGTGTGGTGTTTAAAATGGCCA
  TGTCCTGAGGAAACTTAAGTAACAAAGTACTAAATGCTAAGTAGGCTTTTGCATATTGTAACTAAATTTA
  AGAATAATTCAGATTAAGTAGTTCTGAAATTTGGTATAGATAGCATAGATTGTCTCATGCTCATGAGTGA
  CATAATGACCCTGGATTCTGTTACATACTTCTAAAGAAAATTGATTGTTGTCTTAGGAGGCAGTTAACTT
  GGCTGAACACCAACTCCACACTCTGTCTTGTTTGTAGGTGGCAGCAGCTGAAATCTCTTCTCAGTTGTTT
  TAGCTTTAGCTATGCTGCTGGAAGTCTTTCCCATGCAAGTGTGTAGTTCAGGGGTCAACCAGAGTTTGGG
  CAGAAGGAAGTCTGCCCCTTCTGTGCCTCCTGTTTTTTGGGGGTTTCCCCTTTATGTTCCAGCTGTTGTG
  GTTGCCCCATATTCTGCCTTCTGATCCTTAACCAATAAAACTTGGCTTTTGTTTCCCCCTCAAGTGAGAA
  CCCGTTAAAAATGAGACATTGAGCCAGTGCTGTTCACTTTTTAAGTGCCAACTTCCCTCTACTTTCCACT
  TGTTTATAGTTGTTTCCAGTGCCTTTAGTTTTTTCTAAAATATATTTGTTCAGAGTTTGCAGTTGCTATC
  AGCAGGAGGGTTGGTCTGATATCTGTGTGCTACTTTGCCATTATTGGAAGTGAACTCTGCATCTTTTTAA
  AAATTTGAAATCCCGGTATCATGTGAAGTGCTGTTTATGTAAATCTCAACATATCCCTTACTCAGGGAAA
  AAAAAGTTTTTAGTTAGGGAATAGTGAAATATAATTTAATATGGAATTCTAGCTGTAGAGTTAAATCCAT
  CTTTAAGTGTTTACATTCAGTATGAGAATGCAAATTTATCTGTATGGGGAATAAAGTCCTAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000108.3
• Summary: 'This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'
• Locus ID: 1738