MAT1A (NM_000429) Human 3' UTR Clone

CAT#: SC217281

3`UTR clone of methionine adenosyltransferase I alpha (MAT1A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAT1A
• Synonyms: MAT; MATA1; SAMS; SAMS1
• ACCN: NM_000429
• Insert Size: 1985 bp
• Sequence Data:
  Insert Sequence

  >SC217281 3'UTR clone of NM_000429
  The sequence shown below is from the reference sequence of NM_000429. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTTTAGAGCCAGGGGGAGCTGGGCCTGGT
  CTCACCCTGGAGGCACCTGGTGGCCATGCTCCTCTTCCCCAGACGCCTGGCTGCTGATCGCCTTCCCCAC
  CCACCAACCCTCAGGGCAAAGCCAGGTCCCTCTCATTTAGCCTGTCCTGTCATCATCATGGCCAGCTGGA
  GGCAGGGGCTTCCTGGTGCTGGAGGTTGGATCTTGATGTAAGGATGGGCATGGTGTTCTCCTGCTGCTCC
  CTCAGACTGGGGCAATGTTAATTTAGTGGAAAAGGCACCCCCGTCAAGAGTGAATTCCCTCACTCGTCTC
  CCCCAACAGCTGGACCCTGACCAGCTCCCCCTCCCTCCCCTTGCCTGTGCCAGGTGAGGTCAGCACATCT
  CAACAGGCCTCAGGGCTCCTTGTGGGCCTGGGCTCCTGGACCCCCCTTTCACAGGCAGCCAGTGCCCTGA
  GCCAGGGTCTCCAGAAAGCCCCACCCAGGCCAGGCATGTGGCAGGGGTTAGAGCAGGACTGATGTCTCCT
  AAGCACCTGTAATGTGCGAGGGACCCAGCTAATAACTGATCTCGTTTTTTCTTCACTGCAACATGATGAG
  GTAGTACCTTTTATATCCCATTTATAGATGGGGGAAAGCAAAGCACAGAGAGTCTGGATAACTTCCACAG
  GGTCCCACAGCCACGTGTTTAGACCTAGATGTATAACTAGGAGCTTTGACTCAGGAGCCTGTGACATACC
  CCCTCCCCCACCGTTGTCTCATGCCAGTAACAGGCTCAAACAATGACAAAGCAGATTCAGAAATGAGGCC
  ATGGACTCTGTCCTGAAGGCCTGAGGTTACTGGAAATTAGGGGATTAACCCACTAGCTCTTGTTGAGCCG
  TGGGCAATTGTCTGAAAAGTGAAGACAGAACCACAGGGCTATTTTGTTTGCTTCATGTGTCCCAGAAGAT
  GACTGAGGGTGAGTTGGCTTACCTGGCCCATCAGGGTAGGCTGGAGTTAGGGACTGACCAGCAGCTTTAG
  AATCCCAGCCCCCTGACCACTCAGAGACATGCAGAGATTGGGTTTTTGGACTTCTGGGGTAAGTGGTCTA
  AGTCCAGTCCAGTCCTATCTGGGCTTCCTGGAGCAGAAGCAGCAACTTGTCCTAGCACAGATGGCCAGCC
  CCTTAGACAGAGGCCCTCAAGTCTTTCTCTTTCCCTGGTCCCTTGTATCCCCTGCAGGCTGAGTGCATTT
  GGAGGGAGTGAGTGGCCCTTTCGGATCCAGGGAGGCTGGTCCTATGGCCTCATGTTAAATAGGCGGGGCT
  TGCCTTCTGGTGTTGGACAAGCTTCTGAGACGTCATGAGGAGATTCTGCCTTTGCCAGGTGACTGTCTGG
  GGAGCGGGTCTGCTCCCAAGGGGCCTGAGCAGTCCTTGGCCTGCTAAGGTCTTGGAACTTGCCTGCCTTT
  CCATCCATGGCCAGCAGCACCTGCCCTACCTGCCCCACTTGTCCTTAGCCTGGACCTCTGACAGCAGCAT
  CTCTACCTTCTCCCCAGCTCCCAGGACCACAGGCTCAGGCAGGGGCCTCCATGGGCCCCAGGGGAACACT
  GGGGACTTGGCCTCTCTCTAGGGTACATGGTGCTGGGAGAGGCAGCCCAGGAAGTCTCATCTGGGGAGCA
  GGCAGCCAGCATCTGGGCCTTGGCCTGGAGCACAAAGACCCTGGCTTTCATTTTCTCTCAGGTGAAAGGA
  AATTAAGGCAACAAAAGAAGCCCGGCTCCTGGTCACCTAGGAAGCCTCAGATTCCTTCCCATGGAGGGAG
  GGAGTGGTTTGCAGGTGGCCAAGTTCCTCTAACTTGGCTCACACTCGACATGAAAATTCAGAATTTTATA
  CTTTCCCTACCCTCTAGAGAAATAAGATCTTTTTTGTCAGTTTGTTTGTATGAAACTAAAGCCTTTATTT
  GTTAATAGTTCCTGCTAAAACAATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000429.2
• Summary: 'This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]'
• Locus ID: 4143