Raptor (RPTOR) (NM_020761) Human 3' UTR Clone

CAT#: SC217490

3`UTR clone of regulatory associated protein of MTOR complex 1 (RPTOR) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RPTOR
• Synonyms: KOG1; Mip1
• ACCN: NM_020761
• Insert Size: 2060
• Sequence Data:
  Insert Sequence

  >SC217490 3'UTR clone of NM_020761
  The sequence shown below is from the reference sequence of NM_020761. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACATCTCCGTGTACTCGGTGGAGAAGCGTGTCAGATAGCGGCGTGACCCGGGCCCACCAGGCCACGGCCG
  CCTGCTGTACATAGTGAAGCTGTCACTCGCCGGGGCACGGGGCGTCGGCTGCTGCGGCCCCGCAGTGTGA
  ACGTTGGCTGCTGCCTTAGCTGCTGATGACGGCAGGAGGGCCCTGCTACTCGCTTTTGTCTGTCTTCGCT
  GTCGTGTCTGGAATGTCAGGGAAGGGGAGGGCTCGGGTTGACGGTGGCTTCCCACTGAGCACCAGCATCC
  AGGTGCACCCCCGCGGCCACGGCGCCTCTGTCCCTCTCCTGTTCTGTGTTTCTCTGAGACGCTGAAAGGG
  GAAACACCTCACTTTATTTCCATGTAATCAGAGCATTAGCTGCAGAAAAACCCCCCGACAGAGCCCTGGC
  GGAGAGGCAGGCGCTGGGGCTCCTACGGGTCCCTGGGGCAGCTGTCCCCATCAGGCCAAGAGCGAGCGAG
  AGGCGCTGCCCCAGCCAGGCCCACCACCTCTCACAGTCAGTGCACGCAAGCAGGGACATTTCCTAGCCAG
  CTGGGGGACACTGGAAATTCGGGAAACCAAGAGAGAGGAAGAAGGAGACGCCCCTCCAACTGGCGGGTGT
  GAAGGAAGCCGCCCAGGGGTCCGGGCTGTCCTTGGCCGCTGGCAGCATCACTGAGCAGGAAGCGCACAGC
  CCACCCTCCCCGCACCTCCAGGTCTCTGGACTCCAGTTTTGGCCCCTCTCACACAGAGCTGTCAGCAGGG
  GCCGCTGTGGCGGTGCACAGGGGAGGCAGGTCCTTGGCGAGGTAGCCCCTGCCTTAATCCACGGGGCTCC
  TTTCCCTCCGAAGGGCTGCTCTTCCCCACAGGCGCGGGGACAGCAGCCCGACCTGTGGTCTCCATGCCTG
  TGCCCTCACACAGGTGTAGCACACGCATGTGCAGATGGCACCACGGCCGGCACCTGGGGGCACACACATG
  CAGGCGGCGTGGTCTCCCTGCTCTGTCCCCACACGTTCCTCACATACAGGCAAGAGGCACTGCCGGGTCC
  CGGACGGCTCCGGGTGACACCAGCCCCGTCTCCAGCCTTGAGCCGCCCATGCTGATGCGACCTCGGCTGA
  CAGCTGGGCCTGTGGTGCAGACAGGAGCTGTGTGGACAGTCCCGCCCAGGAGGGGCCGCAGGGCGTGTAT
  GAGCAGTTTTGCAAACAGAACACAACCACAATGATGGTATTTTGAAAAGTGTTCTTTCCGTGTTCGTCGG
  GAATCAGGATTATTGAGAGGTGAAGGAGCCAGGTGGCTTCATTCTGGCGGTGAGAGGCCCACGACCACGG
  GAGTGAGAGCTGGTGTGGCGAGGCCCGGCTCTCCTGCGGTGTGGCTGGTGGCCTGCCGTGGCCAAGAGCA
  TCTTCTGGGTGGATGGAACCCTGCCTGGTCACATTTGGCCAGAGACACACCTGGCCCTCAGGGGGCTGAG
  CTGGAGACTGAGCTGGGGCTGGCCGGGACGTGACAAGGCAGGACAGAGGCGGCCCCTCCGCTGCTCCTTT
  TTGGAATGCGAGCTCCCACCAGAAGAAGGTTCCGGCACGAATCCCATCCCCACGTCTGGGCCGAGAAAGC
  AGCCCGGGTCCGGAAGGTGTAGAGAGTCCCGGCCTCACTCAGCTCACAGGGCGTGCCAGGCGGCAACACC
  AGAATCTTCCAGAAGCCCAGCTCCACCCGCACACGCAGCTTCCCATCCAGTCCTTCAACTCAATTCTTAC
  CCAACACGCGTTTCTGTTTGTTTTGAGACAAAATCACCACCTGTCAAAAGGCAGGTGGCTCCAGAGGGGT
  CAAGACCCCCCCCCGCCCCCGCTCCACCCTGGAGCCCACCCCCATGGGCACCGCGTGCCGCCTGCACGTG
  GGCTGTCTTCACAGGTCTGATGTGAAAATTCAATCACGACGTTAACCGGCTCGAGAGAGCGCCGGCCTAG
  AGGCTCATTATCTATTTATTTTACCAAACGCGAATTGAGACGGACTTTGACAAAACACGAAATGGTAATG
  TGAAGCTAAGAGCAGAGAGTGACCAACAGT
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_020761.2
• Summary: This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
• Locus ID: 57521