Endothelin A Receptor (EDNRA) (NM_001957) Human 3' UTR Clone

CAT#: SC218505

3`UTR clone of endothelin receptor type A (EDNRA) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EDNRA
• Synonyms: ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA
• ACCN: NM_001957
• Insert Size: 2335 bp
• Sequence Data:
  Insert Sequence

  >SC218505 3'UTR clone of NM_001957
  The sequence shown below is from the reference sequence of NM_001957. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGCCATAAGGACAGCATGAACTGACCACCCTTAGAAGCACTCCTCGGTACTCCCATAATCCTCTCGGA
  GAAAAAAATCACAAGGCAACTGTGAGTCCGGGAATCTCTTCTCTGATCCTTCTTCCTTAATTCACTCCCA
  CACCCAAGAAGAAATGCTTTCCAAAACCGCAAGGGTAGACTGGTTTATCCACCCACAACATCTACGAATC
  GTACTTCTTTAATTGATCTAATTTACATATTCTGCGTGTTGTATTCAGCACTAAAAAATGGTGGGAGCTG
  GGGGAGAATGAAGACTGTTAAATGAAACCAGAAGGATATTTACTACTTTTGCATGAAAATAGAGCTTTCA
  AGTACATGGCTAGCTTTTATGGCAGTTCTGGTGAATGTTCAATGGGAACTGGTCACCATGAAACTTTAGA
  GATTAACGACAAGATTTTCTACTTTTTTTAAGTGATTTTTTTGTCCTTCAGCCAAACACAATATGGGCTC
  AAGTCACTTTTATTTGAAATGTCATTTGGTGCCAGTATTTTTTAACTGCATAATAGCCTAACATGATTAT
  TTGAACTTATTTACACATAGTTTGAAAAAAAAAAGACAAAAATAGTATTCAGGTGAGCAATTAGATTAGT
  ATTTTCCACGTCACTGTTTATTTTTTTAAAACACAAATTCTAAAGCTACAACAAATACTACAGGCCCTTA
  AAGCACAGTCTGATGACACATTTGGCAGTTTAATAGATGTTACTCAAAGAATTTTTTAAGAACTGTATTT
  TATTTTTTAAATGGTGTTTTATTACAAGGGACCTTGAACATGTTTTGTATGTTAAATTCAAAAGTAATGC
  TTCAATCAGATAGTTCTTTTTCACAAGTTCAATCTGTTTTTCATGTAAATTTTGTATGAAAAATCAATGT
  CAAGTACCAAAATGTTAATGTATGTGTCATTTAACTCTGCCTGAGACTTTCAGTGCACTGTATATAGAAG
  TCTAAAACACACCTAAGAGAAAAAGATCGAATTTTTCAGATGATTCAGAAATTTTCATTCAGGTATTTGT
  AATAGTGACATATATATGTATATACATATCACCTCCTATTCTCTTAATTTTTCTTAAAATGTTAACTGGC
  AGTAAGTCTTTTTTGATCATTCCCTTTTCCATATAGGAAACATAATTTTGAAGTGGCCAGATGAGTTTAT
  CATGTCAGTGAAAAATAATTACCCACAAATGCCACCAGAACTTACGATTCTTCACTTCTTGGGGTTTTCA
  GTATGAACCTAACTCCCCACCCCAACATCTCCCTCCCACATTGTCACCATTTCAAAGGGCCCACAGTGAC
  TTTTGCTGGGCATTTTCCCAGATGTTTACAGACTGTGAGTACAGCAGAAAATCTTTTACTAGTGTGTGTG
  TGTATATATATAAACAATTGTAAATTTCTTTTAGCCCATTTTTCTAGACTGTCTCTGTGGAATATATTTG
  TGTGTGTGATATATGCATGTGTGTGATGGTATGTATGGATTTAATCTAATCTAATAATTGTGCCCCGCAG
  TTGTGCCAAAGTGCATAGTCTGAGTAAAATCTAGGTGATTGTTCATCATGACAACCTGCCTCAGTCCATT
  TTAACCTGTAGCAACCTTCTGCATTCATAAATCTTGTAATCATGTTACCATTACAAATGGGATATAAGAG
  GCAGCGTGAAAGCAGATGAGCTGTGGACTAGCAATATAGGGTTTTGTTTGGTTGGTTGGTTTGATAAAGC
  AGTATTTGGGGTCATATTGTTTCCTGTGCTGGAGCAAAAGTCATTACACTTTGAAGTATTATATTGTTCT
  TATCCTCAATTCAATGTGGTGATGAAATTGCCAGGTTGTCTGATATTTCTTTCAGACTTCGCCAGACAGA
  TTGCTGATAATAAATTAGGTAAGATAATTTGTTGGGCCATATTTTAGGACAGGTAAAATAACATCAGGTT
  CCAGTTGCTTGAATTGCAAGGCTAAGAAGTACTGCCCTTTTGTGTGTTAGCAGTCAAATCTATTATTCCA
  CTGGCGCATCATATGCAGTGATATATGCCTATAATATAAGCCATAGGTTCACACCATTTTGTTTAGACAA
  TTGTCTTTTTTTCAAGATGCTTTGTTTCTTTCATATGAAAAAAATGCATTTTATAAATTCAGAAAGTCAT
  AGATTTCTGAAGGCGTCAACGTGCATTTTATTTATGGACTGGTAAGTAACTGTGGTTTACTAGCAGGAAT
  ATTTCCAATTTCTACCTTTACTACATCTTTTCAACAAGTAACTTTGTAGAAATGAGCCAGAAGCCAAGGC
  CCTGAGTTGGCAGTGGCCCATAAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001957.3
• Summary: 'This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
• Locus ID: 1909