PDGF Receptor alpha (PDGFRA) (NM_006206) Human 3' UTR Clone

CAT#: SC220069

3`UTR clone of platelet-derived growth factor receptor alpha polypeptide (PDGFRA) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDGFRA
• Synonyms: CD140A; PDGFR-2; PDGFR2
• ACCN: NM_006206
• Insert Size: 2992 bp
• Sequence Data:
  Insert Sequence

  >SC220069 3'UTR clone of NM_006206
  The sequence shown below is from the reference sequence of NM_006206. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCTTCAGACCTGGTGGAAGACAGCTTCCTGTAACTGGCGGATTCGAGGGGTTCCTTCCACTTCTGGGGC
  CACCTCTGGATCCCGTTCAGAAAACCACTTTATTGCAATGCAGAGGTTGAGAGGAGGACTTGGTTGATGT
  TTAAAGAGAAGTTCCCAGCCAAGGGCCTCGGGGAGCGTTCTAAATATGAATGAATGGGATATTTTGAAAT
  GAACTTTGTCAGTGTTGCCTCTTGCAATGCCTCAGTAGCATCTCAGTGGTGTGTGAAGTTTGGAGATAGA
  TGGATAAGGGAATAATAGGCCACAGAAGGTGAACTTTGTGCTTCAAGGACATTGGTGAGAGTCCAACAGA
  CACAATTTATACTGCGACAGAACTTCAGCATTGTAATTATGTAAATAACTCTAACCAAGGCTGTGTTTAG
  ATTGTATTAACTATCTTCTTTGGACTTCTGAAGAGACCACTCAATCCATCCATGTACTTCCCTCTTGAAA
  CCTGATGTCAGCTGCTGTTGAACTTTTTAAAGAAGTGCATGAAAAACCATTTTTGAACCTTAAAAGGTAC
  TGGTACTATAGCATTTTGCTATCTTTTTTAGTGTTAAAGAGATAAAGAATAATAATTAACCAACCTTGTT
  TAATAGATTTGGGTCATTTAGAAGCCTGACAACTCATTTTCATATTGTAATCTATGTTTATAATACTACT
  ACTGTTATCAGTAATGCTAAATGTGTAATAATGTAACATGATTTCCCTCCAGAGAAAGCACAATTTAAAA
  CAATCCTTACTAAGTAGGTGATGAGTTTGACAGTTTTTGACATTTATATTAAATAACATGTTTCTCTATA
  AAGTATGGTAATAGCTTTAGTGAATTAAATTTAGTTGAGCATAGAGAACAAAGTAAAAGTAGTGTTGTCC
  AGGAAGTCAGAATTTTTAACTGTACTGAATAGGTTCCCCAATCCATCGTATTAAAAAACAATTAACTGCC
  CTCTGAAATAATGGGATTAGAAACAAACAAAACTCTTAAGTCCTAAAAGTTCTCAATGTAGAGGCATAAA
  CCTGTGCTGAACATAACTTCTCATGTATATTACCCAATGGAAAATATAATGATCAGCAAAAAGACTGGAT
  TTGCAGAAGTTTTTTTTTTTTTTTTCTTCATGCCTGATGAAAGCTTTGGCGACCCCAATATATGTATTTT
  TTGAATCTATGAACCTGAAAAGGGTCAGAAGGATGCCCAGACATCAGCCTCCTTCTTTCACCCCTTACCC
  CAAAGAGAAAGAGTTTGAAACTCGAGACCATAAAGATATTCTTTAGTGGAGGCTGGATGTGCATTAGCCT
  GGATCCTCAGTTCTCAAATGTGTGTGGCAGCCAGGATGACTAGATCCTGGGTTTCCATCCTTGAGATTCT
  GAAGTATGAAGTCTGAGGGAAACCAGAGTCTGTATTTTTCTAAACTCCCTGGCTGTTCTGATCGGCCAGT
  TTTCGGAAACACTGACTTAGGTTTCAGGAAGTTGCCATGGGAAACAAATAATTTGAACTTTGGAACAGGG
  TTGGCATTCAACCACGCAGGAAGCCTACTATTTAAATCCTTGGCTTCAGGTTAGTGACATTTAATGCCAT
  CTAGCTAGCAATTGCGACCTTAATTTAACTTTCCAGTCTTAGCTGAGGCTGAGAAAGCTAAAGTTTGGTT
  TTGACAGGTTTTCCAAAAGTAAAGATGCTACTTCCCACTGTATGGGGGAGATTGAACTTTCCCCGTCTCC
  CGTCTTCTGCCTCCCACTCCATACCCCGCCAAGGAAAGGCATGTACAAAAATTATGCAATTCAGTGTTCC
  AAGTCTCTGTGTAACCAGCTCAGTGTTTTGGTGGAAAAAACATTTTAAGTTTTACTGATAATTTGAGGTT
  AGATGGGAGGATGAATTGTCACATCTATCCACACTGTCAAACAGGTTGGTGTGGGTTCATTGGCATTCTT
  TGCAATACTGCTTAATTGCTGATACCATATGAATGAAACATGGGCTGTGATTACTGCAATCACTGTGCTA
  TCGGCAGATGATGCTTTGGAAGATGCAGAAGCAATAATAAAGTACTTGACTACCTACTGGTGTAATCTCA
  ATGCAAGCCCCAACTTTCTTATCCAACTTTTTCATAGTAAGTGCGAAGACTGAGCCAGATTGGCCAATTA
  AAAACGAAAACCTGACTAGGTTCTGTAGAGCCAATTAGACTTGAAATACGTTTGTGTTTCTAGAATCACA
  GCTCAAGCATTCTGTTTATCGCTCACTCTCCCTTGTACAGCCTTATTTTGTTGGTGCTTTGCATTTTGAT
  ATTGCTGTGAGCCTTGCATGACATCATGAGGCCGGATGAAACTTCTCAGTCCAGCAGTTTCCAGTCCTAA
  CAAATGCTCCCACCTGAATTTGTATATGACTGCATTTGTGTGTGTGTGTGTGTTTTCAGCAAATTCCAGA
  TTTGTTTCCTTTTGGCCTCCTGCAAAGTCTCCAGAAGAAAATTTGCCAATCTTTCCTACTTTCTATTTTT
  ATGATGACAATCAAAGCCGGCCTGAGAAACACTATTTGTGACTTTTTAAACGATTAGTGATGTCCTTAAA
  ATGTGGTCTGCCAATCTGTACAAAATGGTCCTATTTTTGTGAAGAGGGACATAAGATAAAATGATGTTAT
  ACATCAATATGTATATATGTATTTCTATATAGACTTGGAGAATACTGCCAAAACATTTATGACAAGCTGT
  ATCACTGCCTTCGTTTATATTTTTTTAACTGTGATAATCCCCACAGGCACATTAACTGTTGCACTTTTGA
  ATGTCCAAAATTTATATTTTAGAAATAATAAAAAGAAAGATACTTACATGTTCCCAAAACAATGGTGTGG
  TGAATGTGTGAGAAAAACTAACTTGATAGGGTCTACCAATACAAAATGTATTACGAATGCCCCTGTTCAT
  GTTTTTGTTTTAAAACGTGTAAATGAAGATCTTTATATTTCAATAAATGATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006206.4
• Summary: 'This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]'
• Locus ID: 5156