CYP1B1 (NM_000104) Human 3' UTR Clone

CAT#: SC220331

3`UTR clone of cytochrome P450 family 1 subfamily B polypeptide 1 (CYP1B1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP1B1
• Synonyms: ASGD6; CP1B; CYPIB1; GLC3A; P4501B1
• ACCN: NM_000104
• Insert Size: 3116 bp
• Sequence Data:
  Insert Sequence

  >SC220331 3'UTR clone of NM_000104
  The sequence shown below is from the reference sequence of NM_000104. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACAAGCCAAGGAAACTTGCCAATAAGAAGCAAGAGGCAAGCTGAAATTTTAGAAATATTCACATCTTCGG
  AGATGAGGAGTAAAATTCAGTTTTTTTCCAGTTCCTCTTTTGTGCTGCTTCTCAATTAGCGTTTAAGGTG
  AGCATAAATCAACTGTCCATCAGGTGAGGTGTGCTCCATACCCAGCGGTTCTTCATGAGTAGTGGGCTAT
  GCAGGAGCTTCTGGGAGATTTTTTTGAGTCAAAGACTTAAAGGGCCCAATGAATTATTATATACATACTG
  CATCTTGGTTATTTCTGAAGGTAGCATTCTTTGGAGTTAAAATGCACATATAGACACATACACCCAAACA
  CTTACACCAAACTACTGAATGAAGCAGTATTTTGGTAACCAGGCCATTTTTGGTGGGAATCCAAGATTGG
  TCTCCCATATGCAGAAATAGACAAAAAGTATATTAAACAAAGTTTCAGAGTATATTGTTGAAGAGACAGA
  GACAAGTAATTTCAGTGTAAAGTGTGTGATTGAAGGTGATAAGGGAAAAGATAAAGACCAGAAATTCCCT
  TTTCACCTTTTCAGGAAAATAACTTAGACTCTAGTATTTATGGGTGGATTTATCCTTTTGCCTTCTGGTA
  TACTTCCTTACTTTTAAGGATAAATCATAAAGTCAGTTGCTCAAAAAGAAATCAATAGTTGAATTAGTGA
  GTATAGTGGGGTTCCATGAGTTATCATGAATTTTAAAGTATGCATTATTAAATTGTAAAACTCCAAGGTG
  ATGTTGTACCTCTTTTGCTTGCCAAAGTACAGAATTTGAATTATCAGCAAAGAAAAAAAAAAAAGCCAGC
  CAAGCTTTAAATTATGTGACCATAATGTACTGATTTCAGTAAGTCTCATAGGTTAAAAAAAAAAGTCACC
  AAATAGTGTGAAATATATTACTTAACTGTCCGTAAGCAGTATATTAGTATTATCTTGTTCAGGAAAAGGT
  TGAATAATATATGCCTTGTATAATATTGAAAATTGAAAAGTACAACTAACGCAACCAAGTGTGCTAAAAA
  TGAGCTTGATTAAATCAACCACCTATTTTTGACATGGAAATGAAGCAGGGTTTCTTTTCTTCACTCAAAT
  TTTGGCGAATCTCAAAATTAGATCCTAAGATGTGTTCTTATTTTTATAACATCTTTATTGAAATTCTATT
  TATAATACAGAATCTTGTTTTGAAAATAACCTAATTAATATATTAAAATTCCAAATTCATGGCATGCTTA
  AATTTTAACTAAATTTTAAAGCCATTCTGATTATTGAGTTCCAGTTGAAGTTAGTGGAAATCTGAACATT
  CTCCTGTGGAAGGCAGAGAAATCTAAGCTGTGTCTGCCCAATGAATAATGGAAAATGCCATGAATTACCT
  GGATGTTCTTTTTACGAGGTGACAAGAGTTGGGGACAGAACTCCCATTACAACTGACCAAGTTTCTCTTC
  TAGATGATTTTTTGAAAGTTAACATTAATGCCTGCTTTTTGGAAAGTCAGAATCAGAAGATAGTCTTGGA
  AGCTGTTTGGAAAAGACAGTGGAGATGAGGTCAGTTGTGTTTTTTAAGATGGCAATTACTTTGGTAGCTG
  GGAAAGCATAAAGCTCAAATGAAATGTATGCATTCACATTTAGAAAAGTGAATTGAAGTTTCAAGTTTTA
  AAGTTCATTGCAATTAAACTTCCAAAGAAAGTTCTACAGTGTCCTAAGTGCTAAGTGCTTATTACATTTT
  ATTAAGCTTTTTGGAATCTTTGTACCAAAATTTTAAAAAAGGGAGTTTTTGATAGTTGTGTGTATGTGTG
  TGTGGGGTGGGGGGATGGTAAGAGAAAAGAGAGAAACACTGAAAAGAAGGAAAGATGGTTAAACATTTTC
  CCACTCATTCTGAATTAATTAATTTGGAGCACAAAATTCAAAGCATGGACATTTAGAAGAAAGATGTTTG
  GCGTAGCAGAGTTAAATCTCAAATAGGCTATTAAAAAAGTCTACAACATAGCAGATCTGTTTTGTGGTTT
  GGAATATTAAAAAACTTCATGTAATTTTATTTTAAAATTTCATAGCTGTACTTCTTGAATATAAAAAATC
  ATGCCAGTATTTTTAAAGGCATTAGAGTCAACTACACAAAGCAGGCTTGCCCAGTACATTTAAATTTTTT
  GGCACTTGCCATTCCAAAATATTATGCCCCACCAAGGCTGAGACAGTGAATTTGGGCTGCTGTAGCCTAT
  TTTTTTAGATTGAGAAATGTGTAGCTGCAAAAATAATCATGAACCAATCTGGATGCCTCATTATGTCAAC
  CAGGTCCAGATGTGCTATAATCTGTTTTTACGTATGTAGGCCCAGTCGTCATCAGATGCTTGCGGCAAAA
  GGAAAGCTGTGTTTATATGGAAGAAAGTAAGGTGCTTGGAGTTTACCTGGCTTATTTAATATGCTTATAA
  CCTAGTTAAAGAAAGGAAAAGAAAACAAAAAACGAATGAAAATAACTGAATTTGGAGGCTGGAGTAATCA
  GATTACTGCTTTAATCAGAAACCCTCATTGTGTTTCTACCGGAGAGAGAATGTATTTGCTGACAACCATT
  AAAGTCAGAAGTTTTACTCCAGGTTATTGCAATAAAGTATAATGTTTATTAAATGCTTCATTTGTATGTC
  AAAGCTTTGACTCTATAAGCAAATTGCTTTTTTCCAAAACAAAAAGATGTCTCAGGTTTGTTTTGTGAAT
  TTTCTAAAAGCTTTCATGTCCCAGAACTTAGCCTTTACCTGTGAAGTGTTACTACAGCCTTAATATTTTC
  CTAGTAGATCTATATTAGATCAAATAGTTGCATAGCAGTATATGTTAATTTGTGTGTTTTTAGCTGTGAC
  ACAACTGTGTGATTAAAAGGTATACTTTAGTAGACATTTATAACTCAAGGATACCTTCTTATTTAATCTT
  TTCTTATTTTTGTACTTTATCATGAATGCTTTTAGTGTGTGCATAATAGCTACAGTGCATAGTTGTAGAC
  AAAGTACATTCTGGGGAAACAACATTTATATGTAGCCTTTACTGTTTGATATACCAAATTAAAAAAAAAT
  TGTATCTCATTACTTATACTGGGACACCATTACCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000104.3
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]'
• Locus ID: 1545