MED1 (NM_004774) Human 3' UTR Clone
CAT#: SC220424
3`UTR clone of mediator complex subunit 1 (MED1) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | MED1 |
Synonyms | CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2 |
ACCN | NM_004774 |
Insert Size | 3195 bp |
Sequence Data |
>SC220424 3'UTR clone of NM_004774
The sequence shown below is from the reference sequence of NM_004774. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC ATGGATGTGGCCCTGATTGGGAATTAGGAACCTTATTTCCTAAAAGAAACAGGGCCAGAGGAAAAAAAAC TATTGATAAGTTTATAGGCAAACCACCATAAGGGGTGAGTCAGACAGGTCTGATTTGGTTAAGAATCCTA AATGGCATGGCTTTGACATCAAGCTGGGTGAATTAGAAAGGCATATCCAGACCCTATTAAAGAAACCACA GGGTTTGATTCTGGTTACCAGGAAGTCTTCTTTGTTCCTGTGCCAGAAAGAAAGTTAAAATACTTGCTTA AGAAAGGGAGGGGGGTGGGAGGGGTGTAGGGAGAGGGAAGGGAGGGAAACAGTTTTGTGGGAAATATTCA TATATATTTTCTTCTCCCTTTTTCCATTTTTAGGCCATGTTTTAAACTCATTTTAGTGCATGTATATGAA GGGCTGGGCAGAAAATGAAAAAGCAATACATTCCTTGATGCATTTGCATGAAGGTTGTTCAACTTTGTTT GAGGTAGTTGTCCGTTTGAGTCATGGGCAAATGAAGGACTTTGGTCATTTTGGACACTTAAGTAATGTTT GGTGTCTGTTTCTTAGGAGTGACTGGGGGAGGGAAGATTATTTTAGCTATTTATTTGTAATATTTTAACC CTTTATCTGTTTGTTTTTATACAGTGTTTCGTTCTAAATCTATGAGGTTTAGGGTTCAAAATGATGGAAG GCCGAAGAGCAAGGCTTATATGGTGGTAGGGAGCTTATAGCTTGTGCTAATACTGTAGCATCAAGCCCAA GCAAATTAGTCAGAGCCCGCCTTTAGAGTTAAATATAATAGAAAAACCAAAATGATATTTTTATTTTAGG AGGGTTTAAATAGGGTTCAGAGATCATAGGAATATTAGGAGTTACCTCTCTGTGGAGGTATTGACTTGTA ATCTCATTTTCCTTTCAAAAAAAAAAAAAAAAGCTAAGGTGGCTTGTTGGGATGTAAACATGTTTTCAGA TGCAGTAAGGTTTAGTGTAGGACAGCCTTCCTGACCCAGTGGCATGAAAACCATTACAGGATTAATAGTT CTCCTACTTCCACAATGTGCCAAAAGTCTGCATCCCAGCATTTTGTTTGCAGGAGAACTGATGCCATTCC TAAGAGCTGGACTCACTGTTTCTCTTCATCACAAGGAGAAGGAGTCCAAACTTTAATCACTCCACTGTAT GCTCCCTGAGATAAAACAGTAAAAAATCCGCAGCCATAGTTCACTTAAAACAATTTCAAGCTCACTTTTG AAGTAATGGGGGCCTGGAATGCTAGGTGAGCATGAAGATAAACCCTTGCTACTATGTAGCAACCCAATTG GACCTTTTTGGAGAAATAGGTCTGAGTCTGGATTCTGGGGGACATCAATAAGAGCCCTTCACATAAAAAT ATAGAAATCCAGGAGACTGTTTCGAGTGCAACAGAAGTTCTCAGTATTTGGAGGGTCCTCTCAAAAATTC TGCGGCCTTACTTTGATATTGACACCTGCACTGTGCCATTCCTGATTATTCCATTCAGGATCTGTATCAG CGGGATGGGGCATGGTCCCCAGCACAACTCTTCTGGGTTAAAAAAAAAAAGCCAGGTGATTCCTTTGTGT GTTATGTCGTAAGTGGAGTGACTTCATCATATATGGAAGAAGATTTCTATATTCAGCTTTTTCTGCAGGT TGGAGTCAGCATAGAGTTGGAAAATCAGCTTTGGCTTTCTTTCCTGTCTCATTTCCTCTAGTGTTCTCCT TTTTATTGTCATCAGCTCTCAACAACTCTGCCACTTTTGTGTCCCAAGGTAATAAGATGTAGGAAACAAA ACATTGTAAAGTGGAGCAAGAAAAGTTATCAATTAACCACATCAGAGTCAAATGTCTTGGGTGACACTAA GGAGGATATGGGCAGGTGATACCAGAGTGCTTTATCTTGTGATGTTGATACAGTAGCAGCCTCTCAGACA TTCAGCCAGGTTGGATTTCTCATGAGTTTGTCACCTAGTTTTGAATCCTATCCCGTTGGTTTCTGCAGGA AAAAAAAAAAAATTTATGTGGTTTTTAAAATTTGTTCTGAGTGGGGAGAATCTTAGGGGGAATGTACTGA ATAGTATCATGGGCTCAGCTCCCCCATGCAGGGCCAACAAATACCAAAATGAGTAAACTGGGAAGCTTTT CTCTCTTTCTGTCTTCATCCCAGATCAAAGAATCCCGAGTTAGGATCTGGATGAAGGATAAGCCCCTGAA TTGTCGATGGGCTCACCCCCACACTGACCCAGCATCTGAACTTGCTTAACAGGGAGCCGGGGCTAAACTG CTTCACCCTGCCTGAGAACCAGGGAGCACTGCATTTCTCCACAGGGTGGAGGAGAAGAGGCAGAATAAAC CAAGCCTGGGACACCTCCCTCCTGTCTAGGTGTACTCATTCTTCTGTTTCAAAAGAAGGCAAGGACATGA AGTCAACTTCTACCTATCTTCTGCTGCTGGTGTCTTATGTATTCTCAGTTTGACCTGATTCCTCTTCTGT CTTTTGACTTAACATTAGGGGTTCTTGGTCATAACCTGCTCTGATGTACATAAAGATTTCAGGTTCAATA TCAATGTGTCTTAAAACAGAAGTATTTTAGCGGGTGGGGGGTGGGGTGGTGGGGACAAACAACGCAGGAT ATAATTGCCAAAACCAGGCTTGAGGTTGGTGACTCTTGAAAGATTTTCTTTCTTCAGGCCTAGATCAGAA AATTAAGTGCAGCAATATCATGAATTCTCAGAAGCCCTTTCAGGGAGCCAGTGAGTCATACAGTATCCAC AGTTGAGTCACTTAAAGATGTCAGTATACGAAACATTATTCACAATCCTTGGGCAATCTCATTTTTTTTT CCTTCTCCCCTCCTCCCCTGCCCCCCATACATTTCTATCCTTGAGTTAGTTTTGGAGGGGCAGGAAGTAC TTAACATCTCAGAAGCTAGATTGGGAAACATGCTCAGCTATAAGAACTGAGCTTTAAATTTTGAGTTTAA AAATGTACATCAGGAGCAGCTGGGGAGGGTCTTTTTTTAAAAAAATCTTTCAAATTTGGTTTTCTGTGCA TATGGCCGTTTTGTAAATACTTTGGGGTTTTTCATTTTTTTGAAAGTAGATGAAATCTGTTGTGGGATTT TTTTCCCGAAACATTACAAAATAACCTGTTTATTTACATGCAAAT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_004774.3 |
Summary | 'The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]' |
Locus ID | 5469 |
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