MED1 (NM_004774) Human 3' UTR Clone

CAT#: SC220424

3`UTR clone of mediator complex subunit 1 (MED1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MED1
• Synonyms: CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
• ACCN: NM_004774
• Insert Size: 3195 bp
• Sequence Data:
  Insert Sequence

  >SC220424 3'UTR clone of NM_004774
  The sequence shown below is from the reference sequence of NM_004774. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGGATGTGGCCCTGATTGGGAATTAGGAACCTTATTTCCTAAAAGAAACAGGGCCAGAGGAAAAAAAAC
  TATTGATAAGTTTATAGGCAAACCACCATAAGGGGTGAGTCAGACAGGTCTGATTTGGTTAAGAATCCTA
  AATGGCATGGCTTTGACATCAAGCTGGGTGAATTAGAAAGGCATATCCAGACCCTATTAAAGAAACCACA
  GGGTTTGATTCTGGTTACCAGGAAGTCTTCTTTGTTCCTGTGCCAGAAAGAAAGTTAAAATACTTGCTTA
  AGAAAGGGAGGGGGGTGGGAGGGGTGTAGGGAGAGGGAAGGGAGGGAAACAGTTTTGTGGGAAATATTCA
  TATATATTTTCTTCTCCCTTTTTCCATTTTTAGGCCATGTTTTAAACTCATTTTAGTGCATGTATATGAA
  GGGCTGGGCAGAAAATGAAAAAGCAATACATTCCTTGATGCATTTGCATGAAGGTTGTTCAACTTTGTTT
  GAGGTAGTTGTCCGTTTGAGTCATGGGCAAATGAAGGACTTTGGTCATTTTGGACACTTAAGTAATGTTT
  GGTGTCTGTTTCTTAGGAGTGACTGGGGGAGGGAAGATTATTTTAGCTATTTATTTGTAATATTTTAACC
  CTTTATCTGTTTGTTTTTATACAGTGTTTCGTTCTAAATCTATGAGGTTTAGGGTTCAAAATGATGGAAG
  GCCGAAGAGCAAGGCTTATATGGTGGTAGGGAGCTTATAGCTTGTGCTAATACTGTAGCATCAAGCCCAA
  GCAAATTAGTCAGAGCCCGCCTTTAGAGTTAAATATAATAGAAAAACCAAAATGATATTTTTATTTTAGG
  AGGGTTTAAATAGGGTTCAGAGATCATAGGAATATTAGGAGTTACCTCTCTGTGGAGGTATTGACTTGTA
  ATCTCATTTTCCTTTCAAAAAAAAAAAAAAAAGCTAAGGTGGCTTGTTGGGATGTAAACATGTTTTCAGA
  TGCAGTAAGGTTTAGTGTAGGACAGCCTTCCTGACCCAGTGGCATGAAAACCATTACAGGATTAATAGTT
  CTCCTACTTCCACAATGTGCCAAAAGTCTGCATCCCAGCATTTTGTTTGCAGGAGAACTGATGCCATTCC
  TAAGAGCTGGACTCACTGTTTCTCTTCATCACAAGGAGAAGGAGTCCAAACTTTAATCACTCCACTGTAT
  GCTCCCTGAGATAAAACAGTAAAAAATCCGCAGCCATAGTTCACTTAAAACAATTTCAAGCTCACTTTTG
  AAGTAATGGGGGCCTGGAATGCTAGGTGAGCATGAAGATAAACCCTTGCTACTATGTAGCAACCCAATTG
  GACCTTTTTGGAGAAATAGGTCTGAGTCTGGATTCTGGGGGACATCAATAAGAGCCCTTCACATAAAAAT
  ATAGAAATCCAGGAGACTGTTTCGAGTGCAACAGAAGTTCTCAGTATTTGGAGGGTCCTCTCAAAAATTC
  TGCGGCCTTACTTTGATATTGACACCTGCACTGTGCCATTCCTGATTATTCCATTCAGGATCTGTATCAG
  CGGGATGGGGCATGGTCCCCAGCACAACTCTTCTGGGTTAAAAAAAAAAAGCCAGGTGATTCCTTTGTGT
  GTTATGTCGTAAGTGGAGTGACTTCATCATATATGGAAGAAGATTTCTATATTCAGCTTTTTCTGCAGGT
  TGGAGTCAGCATAGAGTTGGAAAATCAGCTTTGGCTTTCTTTCCTGTCTCATTTCCTCTAGTGTTCTCCT
  TTTTATTGTCATCAGCTCTCAACAACTCTGCCACTTTTGTGTCCCAAGGTAATAAGATGTAGGAAACAAA
  ACATTGTAAAGTGGAGCAAGAAAAGTTATCAATTAACCACATCAGAGTCAAATGTCTTGGGTGACACTAA
  GGAGGATATGGGCAGGTGATACCAGAGTGCTTTATCTTGTGATGTTGATACAGTAGCAGCCTCTCAGACA
  TTCAGCCAGGTTGGATTTCTCATGAGTTTGTCACCTAGTTTTGAATCCTATCCCGTTGGTTTCTGCAGGA
  AAAAAAAAAAAATTTATGTGGTTTTTAAAATTTGTTCTGAGTGGGGAGAATCTTAGGGGGAATGTACTGA
  ATAGTATCATGGGCTCAGCTCCCCCATGCAGGGCCAACAAATACCAAAATGAGTAAACTGGGAAGCTTTT
  CTCTCTTTCTGTCTTCATCCCAGATCAAAGAATCCCGAGTTAGGATCTGGATGAAGGATAAGCCCCTGAA
  TTGTCGATGGGCTCACCCCCACACTGACCCAGCATCTGAACTTGCTTAACAGGGAGCCGGGGCTAAACTG
  CTTCACCCTGCCTGAGAACCAGGGAGCACTGCATTTCTCCACAGGGTGGAGGAGAAGAGGCAGAATAAAC
  CAAGCCTGGGACACCTCCCTCCTGTCTAGGTGTACTCATTCTTCTGTTTCAAAAGAAGGCAAGGACATGA
  AGTCAACTTCTACCTATCTTCTGCTGCTGGTGTCTTATGTATTCTCAGTTTGACCTGATTCCTCTTCTGT
  CTTTTGACTTAACATTAGGGGTTCTTGGTCATAACCTGCTCTGATGTACATAAAGATTTCAGGTTCAATA
  TCAATGTGTCTTAAAACAGAAGTATTTTAGCGGGTGGGGGGTGGGGTGGTGGGGACAAACAACGCAGGAT
  ATAATTGCCAAAACCAGGCTTGAGGTTGGTGACTCTTGAAAGATTTTCTTTCTTCAGGCCTAGATCAGAA
  AATTAAGTGCAGCAATATCATGAATTCTCAGAAGCCCTTTCAGGGAGCCAGTGAGTCATACAGTATCCAC
  AGTTGAGTCACTTAAAGATGTCAGTATACGAAACATTATTCACAATCCTTGGGCAATCTCATTTTTTTTT
  CCTTCTCCCCTCCTCCCCTGCCCCCCATACATTTCTATCCTTGAGTTAGTTTTGGAGGGGCAGGAAGTAC
  TTAACATCTCAGAAGCTAGATTGGGAAACATGCTCAGCTATAAGAACTGAGCTTTAAATTTTGAGTTTAA
  AAATGTACATCAGGAGCAGCTGGGGAGGGTCTTTTTTTAAAAAAATCTTTCAAATTTGGTTTTCTGTGCA
  TATGGCCGTTTTGTAAATACTTTGGGGTTTTTCATTTTTTTGAAAGTAGATGAAATCTGTTGTGGGATTT
  TTTTCCCGAAACATTACAAAATAACCTGTTTATTTACATGCAAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004774.3
• Summary: 'The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]'
• Locus ID: 5469