TGF beta 2 (TGFB2) (NM_003238) Human 3' UTR Clone

CAT#: SC220584

3`UTR clone of transforming growth factor beta 2 (TGFB2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TGFB2
• Synonyms: G-TSF; LDS4; TGF-beta2
• ACCN: NM_003238
• Insert Size: 3252 bp
• Sequence Data:
  Insert Sequence

  >SC220584 3'UTR clone of NM_003238
  The sequence shown below is from the reference sequence of NM_003238. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAAGTCTTGCAAATGCAGCTAAAATTCTTGGAAAAGTGGCAAGACCAAAATGACAATGATGATGATAATG
  ATGATGACGACGACAACGATGATGCTTGTAACAAGAAAACATAAGAGAGCCTTGGTTCATCAGTGTTAAA
  AAATTTTTGAAAAGGCGGTACTAGTTCAGACACTTTGGAAGTTTGTGTTCTGTTTGTTAAAACTGGCATC
  TGACACAAAAAAAGTTGAAGGCCTTATTCTACATTTCACCTACTTTGTAAGTGAGAGAGACAAGAAGCAA
  ATTTTTTTTAAAGAAAAAAATAAACACTGGAAGAATTTATTAGTGTTAATTATGTGAACAACGACAACAA
  CAACAACAACAACAAACAGGAAAATCCCATTAAGTGGAGTTGCTGTACGTACCGTTCCTATCCCGCGCCT
  CACTTGATTTTTCTGTATTGCTATGCAATAGGCACCCTTCCCATTCTTACTCTTAGAGTTAACAGTGAGT
  TATTTATTGTGTGTTACTATATAATGAACGTTTCATTGCCCTTGGAAAATAAAACAGGTGTATAAAGTGG
  AGACCAAATACTTTGCCAGAAACTCATGGATGGCTTAAGGAACTTGAACTCAAACGAGCCAGAAAAAAAG
  AGGTCATATTAATGGGATGAAAACCCAAGTGAGTTATTATATGACCGAGAAAGTCTGCATTAAGATAAAG
  ACCCTGAAAACACATGTTATGTATCAGCTGCCTAAGGAAGCTTCTTGTAAGGTCCAAAAACTAAAAAGAC
  TGTTAATAAAAGAAACTTTCAGTCAGAATAAGTCTGTAAGTTTTTTTTTTTCTTTTTAATTGTAAATGGT
  TCTTTGTCAGTTTAGTAAACCAGTGAAATGTTGAAATGTTTTGACATGTACTGGTCAAACTTCAGACCTT
  AAAATATTGCTGTATAGCTATGCTATAGGTTTTTTCCTTTGTTTTGGTATATGTAACCATACCTATATTA
  TTAAAATAGATGGATATAGAAGCCAGCATAATTGAAAACACATCTGCAGATCTCTTTTGCAAACTATTAA
  ATCAAAACATTAACTACTTTATGTGTAATGTGTAAATTTTTACCATATTTTTTATATTCTGTAATAATGT
  CAACTATGATTTAGATTGACTTAAATTTGGGCTCTTTTTAATGATCACTCACAAATGTATGTTTCTTTTA
  GCTGGCCAGTACTTTTGAGTAAAGCCCCTATAGTTTGACTTGCACTACAAATGCATTTTTTTTTTAATAA
  CATTTGCCCTACTTGTGCTTTGTGTTTCTTTCATTATTATGACATAAGCTACCTGGGTCCACTTGTCTTT
  TCTTTTTTTTGTTTCACAGAAAAGATGGGTTCGAGTTCAGTGGTCTTCATCTTCCAAGCATCATTACTAA
  CCAAGTCAGACGTTAACAAATTTTTATGTTAGGAAAAGGAGGAATGTTATAGATACATAGAAAATTGAAG
  TAAAATGTTTTCATTTTAGCAAGGATTTAGGGTTCTAACTAAAACTCAGAATCTTTATTGAGTTAAGAAA
  AGTTTCTCTACCTTGGTTTAATCAATATTTTTGTAAAATCCTATTGTTATTACAAAGAGGACACTTCATA
  GGAAACATCTTTTTCTTTAGTCAGGTTTTTAATATTCAGGGGGAAATTGAAAGATATATATTTTAGTCGA
  TTTTTCAAAAGGGGAAAAAAGTCCAGGTCAGCATAAGTCATTTTGTGTATTTCACTGAAGTTATAAGGTT
  TTTATAAATGTTCTTTGAAGGGGAAAAGGCACAAGCCAATTTTTCCTATGATCAAAAAATTCTTTCTTTC
  CTCTGAGTGAGAGTTATCTATATCTGAGGCTAAAGTTTACCTTGCTTTAATAAATAATTTGCCACATCAT
  TGCAGAAGAGGTATCCTCATGCTGGGGTTAATAGAATATGTCAGTTTATCACTTGTCGCTTATTTAGCTT
  TAAAATAAAAATTAATAGGCAAAGCAATGGAATATTTGCAGTTTCACCTAAAGAGCAGCATAAGGAGGCG
  GGAATCCAAAGTGAAGTTGTTTGATATGGTCTACTTCTTTTTTGGAATTTCCTGACCATTAATTAAAGAA
  TTGGATTTGCAAGTTTGAAAACTGGAAAAGCAAGAGATGGGATGCCATAATAGTAAACAGCCCTTGTGTT
  GGATGTAACCCAATCCCAGATTTGAGTGTGTGTTGATTATTTTTTTGTCTTCCACTTTTCTATTATGTGT
  AAATCACTTTTATTTCTGCAGACATTTTCCTCTCAGATAGGATGACATTTTGTTTTGTATTATTTTGTCT
  TTCCTCATGAATGCACTGATAATATTTTAAATGCTCTATTTTAAGATCTCTTGAATCTGTTTTTTTTTTT
  TTTAATTTGGGGGTTCTGTAAGGTCTTTATTTCCCATAAGTAAATATTGCCATGGGAGGGGGGTGGAGGT
  GGCAAGGAAGGGGTGAAGTGCTAGTATGCAAGTGGGCAGCAATTATTTTTGTGTTAATCAGCAGTACAAT
  TTGATCGTTGGCATGGTTAAAAAATGGAATATAAGATTAGCTGTTTTGTATTTTGATGACCAATTACGCT
  GTATTTTAACACGATGTATGTCTGTTTTTGTGGTGCTCTAGTGGTAAATAAATTATTTCGATGATATGTG
  GATGTCTTTTTCCTATCAGTACCATCATCGAGTCTAGAAAACACCTGTGATGCAATAAGACTATCTCAAG
  CTGGAAAAGTCATACCACCTTTCCGATTGCCCTCTGTGCTTTCTCCCTTAAGGACAGTCACTTCAGAAGT
  CATGCTTTAAAGCACAAGAGTCAGGCCATATCCATCAAGGATAGAAGAAATCCCTGTGCCGTCTTTTTAT
  TCCCTTATTTATTGCTATTTGGTAATTGTTTGAGATTTAGTTTCCATCCAGCTTGACTGCCGACCAGAAA
  AAATGCAGAGAGATGTTTGCACCATGCTTTGGCTTTCTGGTTCTATGTTCTGCCAACGCCAGGGCCAAAA
  GAACTGGTCTAGACAGTATCCCCTGTAGCCCCATAACTTGGATAGTTGCTGAGCCAGCCAGATATAACAA
  GAGCCACGTGCTTTCTGGGGTTGGTTGTTTGGGATCAGCTACTTGCCTGTCAGTTTCACTGGTACCACTG
  CACCACAAACAAAAAAACCCACCCTATTTCCTCCAATTTTTTTGGCTGCTACCTACAAGACCAGACTCCT
  CAAACGAGTTGCCAATCTCTTAATAAATAGGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003238.2
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]'
• Locus ID: 7042