AKT2 (NM_001626) Human 3' UTR Clone

CAT#: SC220977

3`UTR clone of v-akt murine thymoma viral oncogene homolog 2 (AKT2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AKT2
• Synonyms: HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
• ACCN: NM_001626
• Insert Size: 3567 bp
• Sequence Data:
  Insert Sequence

  >SC220977 3'UTR clone of NM_001626
  The sequence shown below is from the reference sequence of NM_001626. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGTTCTCCTACTCGGCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCC
  ATCACCGCTGGGTGGTTTTTTCCCCCTAACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCCCCACC
  TCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGGCCCCTGCAGCCCCTGC
  CTCCAGCCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGG
  GCTGTTGGGCGGTTGGAGATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCTG
  GGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGCCTCCGCGCTGCTGCATCTGGACGAAG
  GCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGGCTC
  GGAGTGGCCAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAATAAT
  GTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCAGGGCTGGGGGTTAGGGCTGCTCCCTGGTGAATGGAG
  TCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTCCGGCCT
  CCTCCCCACCAAACTGTCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCT
  CCCAGCCCCGTGCCTTACTAGGGCTTCCTTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGGCCTGGCCT
  GGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGGGCCACCAGCCTTGGCT
  GTTACAATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGGTGGGCATCTC
  TGGTACCTATTGGGGTGGGGGGCCTCTGAAAAGGGAGGCTCCTAGGCCCCCCTCACCCCTCCCTCTCCCC
  AGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACGTCCATGTCAGTGCTGTGGGACTCCTG
  TGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGTGTTTGTGT
  GAGGGCAGCGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTGGGCCCCATCCCTGGCCCGAGCATTTCATC
  CTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAGCCCCACTGTGATCCATGAGCTGCCCTGCCCACGCCT
  CCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTGCTCTCCCTATCCCATC
  TCCTGTACTACACAGTTCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTC
  CTCTGTGTGCGATGTTGTTATCTGACAGTTCTCCGTCCCTACTGGCCTTTCTCCTCGTCTTCATATTTGT
  ACGGTACAAGCAATAAAGACACTCATTTCAGACCAGGGCCCAGCCTGCACTCACGCCAGCCCAACCACTC
  TGGGCTTTGCCTTGGTGATGGAGTCAGACCCCTGGGCCCCAGCTCCTCCTGTACTAGCCGTTCCCTTCAG
  CAAGGAGGGCACTGAGCTCAGGGTGAGGGCAGCTGGGGTGTGTGCAGGAGCTCAGGCTGGAGAGGGTGGG
  TGGAGCTGGTGCTGTGGGGCTGAGGGGTATGGGAAGGCTCCCCGCATGTGGGGGTGGGGTGGACAGAGAC
  CACTCCAGGCCCTCAGTGCTGCTTAGGCTAAGAGAGGTGGGGTGGAGGGACAGGGCTGGAAGATCTGGGT
  AGCCCAGAATGAGGAGGGTGCCTGTGCTGTCACTGAATGAGAGGGAGTGGTTCATTCCACCCGGCTGCCG
  AGCCTCAGAGGGGGGCATTCCTATCCTGCCCCACCTCCCTGTTTATGCTGCCACCTGGAAGCCTTGAGGC
  CCCCAAATTCCAGTACAGACCCAGTGGTGTGTTCATGGTGGCGTGGTTGCTGTCACCTGGGAGCTCCTGA
  GCGTTTGGTTAGAACCCTGTTCAGCTTGGGGTCAGCCCTCCCCTAGTCACTGCCCTTTAGCCTGGATGTG
  TCTGGGCCCCTGCACTTCCCGTGCTTGAGTCACGTGGCTGCATGGCCGGGCGCTGGCCGGATGGAACACC
  TCCCCCAGCAAGGGACCAGGGACCAGAGCCCTGGCCTGCCCTGCTGAGCCCTGCTGTGCAGAGGGCCTGG
  CACAGATGAATTTGAGATTTTGCCGCAAGGTGTTAGCACTTCACACCCATTGAGTCTTTGAGATTTTAAG
  TGAATGTAAGCAGAAAAAGTCAGATCCAATTTACAGAAATCAGAGTTAGCTACAGCTAGGACTCGTTTGG
  TTGGGGTTTTTTAGTTTGTCTTTCTAAAGTCATGTGGACCTTAATTTAATTACAAAAGTCTACCCTGGTG
  GTCATAAAATAGGCAGGCCTATGAAGAAAGGCCTTTTACTCTTCCATCTCATCCCAGCCCCGAGTTGACC
  CACGTTGCTGCTCCTCACACCATGGTGATGCAGGTCTCGTAGTGTGGGCACAGGCCTGGCTACCTCATCT
  TTTTAGTGCCTCTCTCCTCTTCCACAGGATGGGGTCCCACAGCTGCAGCAGCTGGCCCCGTAGTTGAGCA
  TGTGTGGTTATCCTGTAGAGCTTTTCCCAAGAAGGGTGTTTGAACTTAGAGTCTTAATAAAATCTTACCA
  AATAAATTTTGAGTAGAATAATCGTCTTTTGCAATGTACATTTTAAAAATTTCACACATTCTTTTTTGTA
  TATAAAGAACAGTGACTGGGCACAGTGGCTCATGCCTGTAATCCCAGCAATTTGGGAGGCCGAGGCGGGC
  GGGTCTCTTGAGGCCAGGGGTTCGAGACCAGCCTGGGCATCATAGGGAGACCTTCATCTCTACAAAAAAT
  ACAAAAATTAGCTGGGCATGGTGGTGCATGCCTGCAATCCCAGCTAACTTGGAAGGCTGAGGTGAGGTGG
  GAAGATCACTTGAGCCCAGGAGTTTGAGGCTGCAGTGAGCTATGATTGCGGCACTGCACTGCAGCCTGGG
  ACAATGAGACTGTGTCTCTAAAAATAAAAAAAAAAAAAACATGATACATGCTATTAAAAAAGACAGCAAA
  GCAGGAGTATAAGAAAGGAAATTCACCCGAGGTCGCAGGGCCTTGAGTACTCATTTTGGTGCTGATTACC
  TCTCTGCAAATGGACACGGCATCATAAATTGGTAGTTTCCTGCTCTTTTTGTGTAATCTTTTCCAGTTAA
  TGTGAAGCCTCTGGGGGCTGCCCTCGTGCACTGATGGTTGTGTGGAGTCGGGGGCGGCAGTGCGATTCCC
  TTTTAGCTGCTGCATGGGGGGAACTCAGGCTTTCCAGCTGCTTCCTGGGGTTCCATGGGGTAGACCCCTC
  AACCGCTTCAGCTGCCCCGTTAACAGGAATTGACTTGGTTTCGTTTGGTGCTACCAGCAGTCCTGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001626.3
• Summary: 'This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019]'
• Locus ID: 208