RUNX2 (NM_001015051) Human 3' UTR Clone

CAT#: SC221230

3`UTR clone of runt-related transcription factor 2 (RUNX2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RUNX2
• Synonyms: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2aA
• ACCN: NM_001015051
• Insert Size: 3800 bp
• Sequence Data:
  Insert Sequence

  >SC221230 3'UTR clone of NM_001015051
  The sequence shown below is from the reference sequence of NM_001015051. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATGAATCTGTTTGGCGACCATATTGAAATTCCTCAGCAGTGGCCCAGTGGTATCTGGGGGCCACATCC
  CACACGTATCAATATATACATATATAGAGAGAGTGCATATATATGTATATCGATTAGCTATCTACAAAGT
  GCCTATTTTTTAGAAGATTTTTCATTCACTCACTCAGTCATGATCTTGCAGCCATAAGAGGGTAGATATT
  GAGAAGCAGAAGGCTCAAGAGAGACAATTGCAATCGAGCTTCAGATTGTTTACTATTTAAGATGTACTTT
  TACAAAGGAACAAAGAAGGGAAAAGGTATTTTTGTTTTTGTTGTTTGGTCTGTTATCATCAATAACCTGT
  TCATATGCCAATTCAGAGAGGTGGACTCCAGGTTCAGGAGGGAGAAGAGCAAAGCCGCTTCCTCTCTGTG
  CTTTGAAACTTCACACCCTCACGGTGGCAGCTGTGTATGGACCAGTGCCCTCCGCAGACAGCTCACAAAA
  CCAGTTGAGGTGCACTAAAGGGACATGAGGTAGAATGGATGCTTCCATCACAGTACCATCATTCAGAATA
  ACTCTTCCAATTTCTGCTTTCAGACATGCTGCAGGTCCTCATCTGAACTGTTGGGTTCGTTTTTTTTTTT
  TTTTTTCCTGCTCCAAGAAAGTGACTTCAAAAATAACTGATCAGGATAGATTATTTTATTTTACTTTTTA
  ACACTCCTTCTCCCCTTTTCCCACTGAACCAAAAAGAAATCCCATCCCTAAAACCTGCCTTCTCCTTTTA
  TGCAAAACTGAAAATGGCAATACATTATTATAGCCATAATGGTATAGATAGTGATTGCGTTTGGCTATGT
  GTTGTTTTCTTTTTTTTTAAATTATGAATATGTGTAAAATCTGAGGTAACTTGCTAACGTGAATGGTCAT
  ATAACTTTAAAGATATATTTATAATTATTTAATGACATTTGGACCCTTGAAACATTTCTTAGTGTATTGA
  TATGTTGACTTCGGTCTCTAAAAGTGCTCTTTATTAAATAACAAATTTCTTCAGTGGTCTAGAGCCATAT
  CTGAAATATTGCTAAGCAATTTCAGTTCATCCAGGCACAATGTGATTTTAAAAAATACTTCCATCTCCAA
  ATATTTTAGATATAGATTGTTTTTGTGATGTATGAAGGAAATGTTATGTTTAGTTCTTTCAGATCTTTGA
  ATGCCTCTAACACAGCTTTGCCTTCTAAAGCGGTAATTAGGGATTTAAAAAACAACCTTTAGCCCTTTAT
  CAGCATGAAATGCTGGAGTGATGTGGTTTTCTAATTTCTTTGGGGTAATTATGACTCTTGTCATATTAAA
  AAGACAAGCACAAGTAAATCATTGAACTACAGAAAAATGTTCTGTGGTTTCATAGTTAAGCAAAACTCTA
  AATCGCCAGGCTTCATAGCAAAGACATAGTCAGCTAAAAGCCGCACATGTGGATAGAGGGTTCAATTATG
  AGACACCTAGTACAGGAGAGCAAAATTGCACCAGAGATTCTTAACCAACCAGCCTTACCAAACAACACAA
  CAGGGGAACCCCAATCTGCCTTACCCAAGGCCCCACTGGCAGCTTTCCACAGAATTTGCATTTAGAGGAG
  CAGAATGACATCACTGTCCTTTGGGAGTAGGTCCTCTGAAAAGGCAGCAGGTTCCAGCAGGTAGCTGAGC
  TGAGAGGACATATGGCCCACGGGGACCTACAGACAGCCTTTGACATTTGTATTTCTTACAATGGAGGGCC
  AAGGAGGGCAAGGGGCTGTGGAGTTTGGTGTCTACTAGTGTGTATGAATTTGAGCTAGAGTCCTTCTGTG
  GCATGCACTTTGACCACTCCTGGCAGTCACATGGCAGATTTCCAAGTGCAAATCCTTAATCCAAACAAGG
  ATCATCTAATGACACCACCAGGCCAATCCCTGCTCTCCTCCCCGAAAAGTCAGGGTCCCTTCATTGGAAT
  CCTCCACCCACCCAAGCAGAATTTAGCAGAGATTTGCCTTCAAACCCTAACGGCCCCCTTGTTCTCTGGT
  CCTTCTCAAACCCACCTTTGTAGGCCACCCAGCATTGCAGGACAGCGTGTGGGGCAGCTGGACCTGTGCT
  TCCTGCCTGGGAGTCTCCCTTGGAATTCATCCTGACTCCTTCTAATAAAAATGGATGGGAAAGCAAAACA
  CTTTGCCTTCTAAAGGCCGTATACCAAGTATGCTTAGATAAATAAGCCACTTTTCTATTACTTAAGTAAG
  AAGGAAGTAGTAATTGATACTATTTATTGTTTGTGTGTGGTAGCTTGAAGCACACCACTGTCCATTTATT
  TGTAAGTGTAAAATATGTGTGTTTGTTTCAGCAGCACTTAAAAAAGCCAGTGTCTGGTTACACATTTCAA
  TTTTAATTAATTGACATAAAAATGCTACCGCCAGTGCCAGCTGCATCCTATTTAATTAAAAAGGTACTAT
  ATTTGTACATTATTTTTTAATGTTAAAAGGGCTTTTTTAAGTTTACAGTACACATACCGAGTGACTTTAG
  GGATGCTTTTGTGTTGAAATGTTACTATAGTGGCTGCAGGCAGCAACCCAGAAACACTTTAGAAGCTTTT
  TTTCCTTGGGAAAAATTCAAGCACTTCTTCCCTCCACCCTCACTCCAACCACCCCAATGGGGGTAATTCA
  CATTTCTTAGAACAAATTCTGCCCTTTTTTGGTCTAGGGATTAAAATTTTGTTTTTCTTTCTTTCTTTTT
  TTTTTTTTTTCACTGAACCCTTAATTTGCACTGGGTCATGTGTTTGATTTGTGATTTCAAGACCAAAGCA
  AAGTCTTACTACTACTGTGGAACCATGTACTAGTTCCTGGGAATTAAAATAGCGTGGTTCTCTTTGTAGC
  ACAAACATTGCTGGAATTTGCAGTCTTTTCAATGCAGCCACATTTTTATCCATTTCAGTTGTCTCACAAA
  TTTTAACCCATATCAGAGTTCCAGAACAGGTACCACAGCTTTGGTTTTAGATTAGTGGAATAACATTCAG
  CCCAGAACTGAGAAACTCAACAGATTAACTATCGTTTGCTCTTTAGACGGTCTCACTGCCTCTCACTTGC
  CAGAGCCCTTTCAAAATGAGCAGAGAAGTCCACACCATTAGGGACCATCTGTGATAAATTCAGAAGGGAG
  GAGATGTGTGTACAGCTTTAAGGATTCCCTCAATTCCGAGGAAAGGGACTGGCCCAGAATCCAGGTTAAT
  ACATGGAAACACGAAGCATTAGCAAAAGTAATAATTATACCTATGGTATTTGAAAGAACAATAATAAAAG
  ACACTTCTTCCAAACCTTGAATTTGTTGTTTTTAGAAAACGAATGCATTTAAAAATATTTTCTATGTGAG
  AATTTTTTAGATGTGTGTTTACTTCATGTTTACAAATAACTGTTTGCTTTTTAATGCAGTACTTTGAAAT
  ATATCAGCCAAAACCATAACTTACAATAATTTCTTAGGTATTCTGAATAAAATTCCATTTCTTTTGGATA
  TGCTTTACCATTCTTAGGTTTCTGTGGAACAAAAATATTTGTAGCATTTTGTGTAAATACAAGCTTTCAT
  TTTTATTTTTTCCAATTGCTATTGCCCAAGAATTGCTTTCCATGCACATATTGTAAAAATTCCGCTTTGT
  GCCACAGGTCATGATTGTGGATGAGTTTACTCTTAACTTCAAAGGGACTATTTGTATTGTATGTTGCAAC
  TGTAAATTGAATTATTTGGCATTTTTCTCATGATTGTAATATTAATTTGAAGTTTGAATTTAATTTTCAA
  TAAAATGGCTTTTTTGGTTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001015051.3
• Summary: 'This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]'
• Locus ID: 860