Mouse Polr2j (NM_011293) AAV Particle

CAT#: MR200521A1V

AAV ORF Particles, serotype AAV-2, Polr2j (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide J (Polr2j), 250ul, >10^13 TU/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Polr2j
• Synonyms: 14.5kDa; Polr2i; Rpb11a; Rpo2-4
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200521 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCTCCTCCGGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGAAAATCACCATTAACAAGG
  ACACTAAGGTTCCCAACGCCTGCTTGTTCACCATCAACAAAGAAGACCACACTCTGGGGAACATCATTAA
  ATCGCAGCTGTTGAAGGACCCGCAGGTGCTGTTTGCTGGCTACAAAGTCCCTCACCCCTTGGAGCACAAG
  ATCATCATTCGTGTGCAGACCACCCCAGACTACAGTCCCCAGGAGGCTTTCACCAACGCCATCACAGACC
  TCATCAGCGAGCTCTCCCTTCTGGAGGAGCGATTCCGGGTGGCCATCAAGGACAAGCAAGAAGGAATTGA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200521 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHK
  IIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_011293
• ORF Size: 354 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_011293.1
• RefSeq Size: 586 bp
• RefSeq ORF: 354 bp
• Locus ID: 20022
• Cytogenetics: 5 G2
• MW: 13.3 kDa