Cdk7 (BC004605) Mouse Untagged Clone

CAT#: MC200160

Cdk7 (untagged) - Mouse cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase) (cDNA clone MGC:6069 IMAGE:3585145),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdk7
• Synonyms: AI323415|AI528512|C230069N13|Cdkn7|Crk4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004605
  CTTTAAATTCCTGCGGGGTGGAGGGCTTAGCTTGGGTGCCCGGGTCTGGTTTCTCACTCGCATGGCTGTG GACGTGAAGTCTCGAGCGAAGCGCTATGAGAAACTGGACTTCCTCGGAGAGGGACAGTTTGCGACGGTCT ATAAGGCCAGGGACAAGAACACCAACCAAATCGTCGCCATTAAGAAAATCAAACTTGGACACAGGTCAGA AGCTAAGGATGGAATAAATAGAACAGCCTTAAGGGAGATAAAGCTCTTACAGGAACTAAGTCATCCAAAT ATAATTGGTCTCCTTGATGCATTTGGACATAAGTCTAACATTAGCCTTGTCTTTGATTTTATGGAAACTG ACCTCGAGGATCTGAAACCAAACAACTTGTTGTTAGATGAGAATGGAGTTCTGAAACTGGCAGATTTTGG CCTGGCCAAATCATTTGGGAGCCCCAATAGGGCTTACACACATCAAGTTGTGACCAGATGGTACCGGGCT CCTGAGTTATTGTTTGGAGCTAGGATGTATGGTGTGGGAGTAGACATGTGGGCTGTTGGTTGTATATTAG CAGAATTGCTTCTAAGGGTTCCATTTTTGCCTGGAGATTCAGATCTTGATCAGCTAACAAGGATATTTGA AACTCTGGGTACACCAACTGAAGAGCAGTGGCCTGACATGTGTAGTCTTCCCGATTATGTGACATTTAAG AGTTTCCCTGGGGTCCCACTGCAGCACATCTTCATCGCGGCTGGGGACGACCTGCTGGAGCTCATCCAAG GCCTGTTCTTATTTAACCCGTGTACGCGGACCACAGCCTCACAGGCATTGAAGACCAAGTACTTCAGTAA CCGCCCCGGGCCAACACCTGGATGCCAGCTCCCGCGACCAAACTGTCCGGTGGAGGCATTAAAGGAACCG GCGAATCCAACCGTGGCAACAAAGCGGAAAAGAGCAGAGGCCTTAGAACAAGGAATATTGCCCAAGAAGC TCATTTTTTAGTTGCAGCGAACAATGGACAGTTTCACTGCTGAAATAAATGATCCAAAGGCAAATAATGG AAAAAATAGTGAATATTAAATGCTATAAGAGAGAACTTGTAAATATTCTACAGATGTAAAATATGTAAAA CTACGGGTTATTTTTATTAAATGTATTTTACAATAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC004605
• ORF Size: 930 bp
• Insert Size: 930
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: BC004605, AAH04605
• RefSeq Size: 1170
• RefSeq ORF: 930
• Locus ID: 12572
• Gene Summary: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. [UniProtKB/Swiss-Prot Function]