Penk (NM_001002927) Mouse Untagged Clone

CAT#: MC209162

Penk (untagged) - Mouse preproenkephalin (Penk), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Penk
• Synonyms: AI326464; ENK; Penk1; PPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209162 representing NM_001002927
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGTTCCTGAGGCTTTGCACCTGGCTGCTGGCGCTTGGGTCCTGCCTCCTGGCTACAGTGCAGG
  CGGAATGCAGCCAGGACTGCGCTAAATGCAGCTACCGCCTGGTTCGCCCAGGCGACATCAATTTCCTGGC
  GTGCACACTGGAATGTGAAGGACAGCTGCCTTCTTTCAAAATCTGGGAGACCTGCAAGGATCTCCTGCAG
  GTGTCCAGGCCCGAGTTCCCTTGGGATAACATCGACATGTACAAAGACAGCAGCAAACAGGATGAGAGCC
  ACTTGCTAGCCAAGAAGTACGGAGGCTTCATGAAACGGTACGGAGGCTTCATGAAGAAGATGGACGAGCT
  ATATCCCATGGAGCCAGAAGAAGAAGCGAACGGAGGAGAGATCCTTGCCAAGAGGTATGGCGGCTTCATG
  AAGAAGGATGCAGATGAGGGAGACACCTTGGCCAACTCCTCCGATCTGCTGAAAGAGCTACTGGGAACGG
  GAGACAACCGTGCGAAAGACAGCCACCAACAAGAGAGCACCAACAATGACGAAGACATGAGCAAGAGGTA
  TGGGGGCTTCATGAGAAGCCTCAAAAGAAGCCCCCAACTGGAAGATGAAGCAAAAGAGCTGCAGAAGCGC
  TACGGGGGCTTCATGAGAAGGGTGGGACGCCCCGAGTGGTGGATGGACTACCAGAAGAGGTATGGGGGCT
  TCCTGAAGCGCTTTGCTGAGTCTCTGCCCTCCGATGAAGAAGGCGAAAATTACTCGAAAGAAGTTCCTGA
  GATAGAGAAAAGATACGGGGGCTTTATGCGGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001002927
• Insert Size: 807 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002927.2, NP_001002927.1
• RefSeq Size: 1442 bp
• RefSeq ORF: 807 bp
• Locus ID: 18619
• Cytogenetics: 4 2.31 cM
• Gene Summary: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. [UniProtKB/Swiss-Prot Function]