Whrn (NM_001008798) Mouse Untagged Clone

CAT#: MC211460

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 9, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; bM340H1.8; C430046P22Rik; mKIAA1526; whirlinNT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211460 representing NM_001008798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATACATTAGACCTGGAAGGAACCGGTGAGACCACCCAGGGCAGCACCAACGCTCTCCCGGATG
  TGTCTGTGGATGATGTCAAATCCCCCTCAGAAGATCTGCCTGGTATCAAGCCACCTCCTCCCCCACCACC
  CCTGGCTCAAGGTCATGACCGCTTGCTTGGCCAGCCACGGAAGCCAGGGAGGGAGGACCCCGCACCTCTG
  TCTTCTGCTGCCCACTCAGGCATTGTCTTCTCAGCACCACGGAACCGCAGCCCGCCGCCAGGGACTGCTC
  CCACTCCGGGACCTTCCTCAGCACAGGACTCTCCCTCCTCCCCCATTTATGCCTCCATCTCCCATGCCAA
  CCCCAGTTCCAGAAAGCCGCTGGACACCCACCTGGCCCTGGTTAACCAGCACCCCATCGGCCCCTTCCCT
  CGAGTCCAGTCCCCACCTCACCTGAAGAGCCCTCCTGCAGAGACCCCAGGAGCTGGGGCCTGCCTTCCAC
  CACCATCACCCTCTGAACACCCTGACGCCGTGGGTGCAAACCAGCACTTTGTTCTGGTGGAGGTGCACCG
  TCCGGACAGTGAGCCTGATGTGAATGAAGTGCGGGCTCTGCCCCAGACTCGCACAGCCTCCACACTCTCT
  CAGCTCTCAGACAGTGGGCAGACCCTGAGCGAGGACAGTGGCGTGGATGCCGGGGAGACGGAGGCCAGCA
  CCTCAGGCCGAGGCAGACAGACAGCATCCGCTAAGAACAAGAATGGCAAGGAGCAGCCCCGGACGGAGAG
  GACCGCGGAGGGTGCCAACAAACCTCCCGGCCTGCTGGAGCCAACGTCCACCCTGGTCCGTGTGAGGAAA
  AGTGCAGCCACACTGGGCATCGCCATTGAGGGCGGTGCCAACACACGCCAGCCTCTGCCCAGGATCGTCA
  CAATTCAGCGAGGAGGTTCTGCCCATAACTGTGGACAGCTCAAGGTGGGCCACGTAATTCTGGAAGTGAA
  TGGGCAGACACTTCGGGGTAAGGAGCACAAGGAGGCCGCCCGAATCATCGCTGAGGCCTTCAAGACCAAG
  GAGAGAGACTACATCGACTTTCTGGTCACTGAGTTCAACGTGATGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008798
• Insert Size: 1101 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008798.1, NP_001008798.1
• RefSeq Size: 2869 bp
• RefSeq ORF: 1101 bp
• Locus ID: 73750
• Cytogenetics: 4 C1
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]