Whrn (NM_001008794) Mouse Untagged Clone

CAT#: MC216522

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; bM340H1.8; C430046P22Rik; mKIAA1526; whirlinNT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216522 representing NM_001008794
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTACTACCTGGCCCAGTACCGTGGTGGCACCATCTCTGTGGAGGCCATGGTCATGGCACTGTTTG
  AGTTGCTCAACACACACGCCAAGTTCTCACTCCTGTCTGAGGTGAGAAGCATCATCTCACCCCAAGACCT
  GGACCGCTTTGACCACCTGGTGCTGAGGCGCGAGATCGAGTCTATGAAGGCGAGGCAGCCCCCCGGCCCC
  GGGGTTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAGGCTCATCCACAGGCAGCCACGGCACCT
  CTACAACCGTCAGCTCGGCCAGGGAGCGGCTGCTGTGGCTTATAGACCTGATGGAGAATACATTAGACCT
  GGAAGGAACCGGTGAGACCACCCAGGGCAGCACCAACGCTCTCCCGGATGTGTCTGTGGATGATGTCAAA
  TCCCCCTCAGAAGATCTGCCTGGTATCAAGCCACCTCCTCCCCCACCACCCCTGGCTCAAGGTCATGACC
  GCTTGCTTGGCCAGCCACGGAAGCCAGGGAGGGAGGACCCCGCACCTCTGTCTTCTGCTGCCCACTCAGG
  CATTGTCTTCTCAGCACCACGGAACCGCAGCCCGCCGCCAGGGACTGCTCCCACTCCGGGACCTTCCTCA
  GCACAGGACTCTCCCTCCTCCCCCATTTATGCCTCCATCTCCCATGCCAACCCCAGTTCCAGAAAGCCGC
  TGGACACCCACCTGGCCCTGGTTAACCAGCACCCCATCGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCA
  CCTGAAGAGCCCTCCTGCAGAGACCCCAGGAGCTGGGGCCTGCCTTCCACCACCATCACCCTCTGAACAC
  CCTGACGCCGTGGGTGCAAACCAGCACTTTGTTCTGGTGGAGGTGCACCGTCCGGACAGTGAGCCTGATG
  TGAATGAAGTGCGGGCTCTGCCCCAGACTCGCACAGCCTCCACACTCTCTCAGCTCTCAGACAGTGGGCA
  GACCCTGAGCGAGGACAGTGGCGTGGATGCCGGGGAGACGGAGGCCAGCACCTCAGGCCGAGGCAGACAG
  ACAGCATCCGCTAAGAACAAGAATGGCAAGGAGCAGCCCCGGACGGAGAGGACCGCGGAGGGTGCCAACA
  AACCTCCCGGCCTGCTGGAGCCAACGTCCACCCTGGTCCGTGTGAGGAAAAGTGCAGCCACACTGGGCAT
  CGCCATTGAGGGCGGTGCCAACACACGCCAGCCTCTGCCCAGGATCGTCACAATTCAGCGAGGAGGTTCT
  GCCCATAACTGTGGACAGCTCAAGGTGGGCCACGTAATTCTGGAAGTGAATGGGCAGACACTTCGGGGTA
  AGGAGCACAAGGAGGCCGCCCGAATCATCGCTGAGGCCTTCAAGACCAAGGAGAGAGACTACATCGACTT
  TCTGGTCACTGAGTTCAACGTGATGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008794
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008794.1, NP_001008794.1
• RefSeq Size: 2665 bp
• RefSeq ORF: 1431 bp
• Locus ID: 73750
• Cytogenetics: 4 C1
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]