Myot (NM_001033621) Mouse Untagged Clone

CAT#: MC217004

Myot (untagged) - Mouse myotilin (Myot), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myot
• Synonyms: 5530402I04Rik; Ttid
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217004 representing NM_001033621
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACTACGAACGTCCAAAACACTTCATCCAGCCGCAAAATCCGTGTGGCTCCAGACTGCAGCCTC
  CAGGACCGGAAGTCTCCGGCTTCCCGAGCCAGACCAAACAGTCTTCCATCGTCATACAGCCTCGCCAGTG
  CACAGAGCAGAGATTTTCTGCCTCCTCCACAGTGAGCTCTCACATCACCGTGTCTTCCTCTGCTTATCCT
  GCTCCCCAGCAGCTTGCTGGCCCCAACCCCGGCCAAAAGGTTACTGCTACCTATAACCAGTCCCCAGCCA
  GCTTCCTCAGTTCTATATTACCGTCTCAACCCGATTACTGTAACAGTAAAATCCCATCCACTGTGGACTC
  CAACTATCAACAATCCTCAGTTAACCAACCTGTAAATGCCATGTCATCCCAAGCTGCAAATGCTAGGCCC
  ACTCCAAAGACTCCTGACCATGAGATCCAAGGATCGAAAGAAGCGTTGATTCAAGATTTGGAAAGAAAGC
  TGAAATGCAAAGACACCCTTCTTCATAATGGAAATCAAAGACTGACCTACGAGGAGAAGATGGCTCGCAG
  GTTGTTAGGACCGCAGAATGCTGCTGCTGTGTTTCAGGCGCAAAACAGTGATGTCCAAGATTCACCACAG
  CATAACCCAGAGCAAGCACGGCTGCACGTTCCCACATCACAAGTAAGAAGTAGATCGTCCTCCAGAGCAG
  AGGCCAATGATCAGGATGCCATCCAGGAGAAGTTTTACCCACCACGCTTCATTCAAGTGCCAGAAAACAT
  GTCGATCGAAGAGGGGAGGTTCTGCCGAATGGACTTTAAAGTAAGTGGACTGCCAGCCCCTGATGTGTCA
  TGGTATCTAAACGGGAGACCAGTGCAATCAGATGAGTTGCACAAAATGATAGTGTCTGAGAAGGGTTTCC
  ACTCCCTCATCTTCGAAGTGGTCAGAGCATCAGATGCAGGGCCATATGCATGCGTGGCCAGGAACAGAGC
  TGGAGAAGCCACCTTCACAGTGCAGCTGGATGTTCTGGCAAAAGAGCATAAAAGAGCACCGATGTTTATC
  TTCAAACCACAGAGTAAGAAAGTTTTTGAGGGAGAGACCGTGAAGCTGGAGTGCCAGATCTCAGCTATCC
  CTCCACCAAAGCTGTTCTGGAAAAGAAACAATGAAATGGTCCAGTTCAACACTGATCGGATAAGCTTATA
  TCATGACAACGCCGGAAGAGTAACTTTACTGATAAAAGATGTAAACAAGAAGGACGCTGGGTGGTATACA
  GTGTCAGCAGTCAATGAAGCTGGTGTGACCACGTGTAACACACGATTAGATGTCACAGCCCGTCCAATCC
  AAACTCTCCCAGCTCCTAAGCAGCTGCGTGTTCGACCAACATTCAGCAAATATTTAGCGCTCAATGGGAG
  AGGTTTGGATGTGAAACAAGCTTTTAACCCTGAAGGAGAATTTCAGCGCCTGGCAGCTCAATCCGGACTC
  TACGAAAGTGAAGAACTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033621
• Insert Size: 1491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033621.3, NP_001028793.1
• RefSeq Size: 2207 bp
• RefSeq ORF: 1491 bp
• Locus ID: 58916
• Cytogenetics: 18 B3