Nsd3 (NM_001001735) Mouse Untagged Clone

CAT#: MC220208

Whsc1l1 (untagged) - Mouse Wolf-Hirschhorn syndrome candidate 1-like 1 (human) (Whsc1l1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsd3
• Synonyms: 6720429E03; A530023P05; AI528490; WHISTLE; Whsc1l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220208 representing NM_001001735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCTCTTTCTCTTTCATGCAAGGGATCATGGGAAACACAATTCAGCAACCACCTCAACTCATTG
  ACTCGGCCAACATCCGCCAGGAGGATGCCTTTGATAACCACAGTGACATTGTTGAAGATGGTGGCCCGAC
  ACCCTTTGAAGCTACTTTGCAACAAGGTTTTCAATATCCACCTACAACAGAAGACCTTCCTCCGCTCACA
  AATGGCTACCCACCATCAATCAGCTTGTATGAAACTCAAACCAAATACCCGCCATATAATCAGTATCCCA
  ATGGGTCAGCCAACGGTTTTGGTGCAGTTAGAAACTTTAGCCCTACTGACTATTACCATTCAGAAATTCC
  AAACACAAGACCACATGAAATTCTGGAAAAACCTTCTCCTCCTCAGCCACCTCCTCTTCCTCCTCCTCCT
  CCTCCTCCTCCTCCTCCTCCTTCGGTACCACAAACTGTGATTCCAAAGAAGACAGGCTCACCCGAGATTA
  AACTAAAAATAACCAAAACTATCCAGAATGGCAGGGAATTGTTTGAGTCTTCCCTTTGTGGAGACCTCTT
  AAATGAAGTACAGGCAAGCGAGCACACAAAGTCTAAGCATGAAAGCAGAAAGGAAAAGAGGAAAAAGAGC
  AACAGACACGAGTCATCTCGATCCGAAGAGCGGAGGTCACACAAGATTCCCAAGCTAGAGCCGGAGGGAC
  AGAATAGACCAAATGAGAGGGTGGACACTGCACCAGAGAAGCCAAGAGAAGAGCCAGTGCTCAAAGAGGC
  CATCCCGGTTCAGCCAATACTGTCTTCTGTTCCAACAACAGAAACATCCACTGGTGTTAAGTTCCAGGTT
  GGTGATCTTGTTTGGTCCAAGGTGGGAACCTACCCTTGGTGGCCTTGTATGGTTTCAAGTGATCCCCAGC
  TTGAGGTCCATTCCAAAATTAACACAAGAGGTGCCCGGGAATATCATGTCCAATTTTTTAGCAACCAGCC
  AGAGAGGGCATGGGTTCATGAGAAACGGGTACGGGAATACAAAGGTCATGAACAGTATGAAGAGTTACTA
  GCCGAGGCAGCCAAGCAAGCCAGCAATCATTCTGAAAAGCAAAAGATTCGGAAACCCCGACCTCAGAGAG
  AACGTGCCCAATGGGACATTGGCATTGCTCATGCAGAGAAAGCATTGAAAATGACTCGGGAGGAAAGAGT
  AGAGCAGTACACTTTCATCTACATTGATAAGCAGCCAGAAGAGGCTTCGTCCCAAGCAAAGAAGAATGTT
  ACCTCTAAGACGGAAGTCAAGAAACCCCGAAGACCAAGATCTGTGCTGAACAGTCAGCCAGAACAGACCA
  ATGCTGGGGAGGTGGCCTCCTCACAATCAAGTACTGACCTTCGAAGGCAGAGCCAGAGGCGGCATACTAG
  CTTGGAAGAGGAAGAGCCACCTCCTGTTAAAATCGCCTGGAAAACAGCAGCCGCAAGGAAGTCCTTACCA
  GCCTCCATCACAATGCACAAAGGGAGCCTAGATTTGCAGAAGTGTAATATGTCTCCAGTTGTGAAAATTG
  AACAAGTGTTTGCTCTCCAGAATGCAACAGGAGATGGGAAGTTTATTGATCAGTTTGTTTATTCAACGAA
  GGGTATTGGTAACAAAACAGAAATAAGTGTCAGGGGGCAAGACAGGCTTATAATCTCTTCACCAAGTCAG
  AGAAGTGAAAAGCCGGCTCAGAGCGCGTCATCTCCGGAGGCAACATCTGGTTCTGCAGGCCCAGTAGAGA
  AGAAGCAGCAGAGAAGATCCATCAGGACTCGATCTGAGTCAGAGAAGTCCGCCGAGGTTGTGCCAAAGAA
  GAAGATCAAAAAGGAGCAGGTTGAGACAGCTCCCCAGGCCTCCCTGAAGACCGGGTTACAGAAAGGGTCG
  GCGGACCGAGGAGCGCAGGGCTCTGTCAGATTCAGTGACAGCTCCGTCTCCGCAGCGAAAGAGGAAACTG
  TGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001735
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001735.2, NP_001001735.1
• RefSeq Size: 3680 bp
• RefSeq ORF: 1968 bp
• Locus ID: 234135
• Cytogenetics: 8 A2
• Gene Summary: This gene encodes a member of the SET domain family of histone lysine N-methyltransferase proteins. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. It acts in opposition to the histone demethylase Jmjd1c. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode the same isoform (1).