Prkg1 (NM_001013833) Mouse Untagged Clone

CAT#: MC220322

Prkg1 (untagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkg1
• Synonyms: AW125416; CGKI; Gm19690; Prkg1b; Prkgr1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220322 representing NM_001013833
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAACTGGAGGAAGACTTTGCGAAGATTCTCATGCTCAAGGAGGAGAGGATCAAAGAGCTGGAGA
  AGCGGCTGTCAGAGAAGGAGGAAGAAATCCAGGAGCTGAAGAGGAAACTCCACAAATGCCAGTCGGTGCT
  GCCGGTGCCCTCCACCCACATCGGCCCGCGGACCACCCGGGCGCAGGGCATCTCCGCGGAGCCGCAGACC
  TACAGGTCCTTCCACGACCTGCGCCAGGCGTTCCGGAAGTTCACTAAATCCGAGAGGTCGAAGGATCTCA
  TAAAGGAGGCTATCCTTGACAATGACTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGA
  CTGTATGTACCCCGTGGAATACGGCAAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTG
  TACGTCATGGAAGATGGGAAGGTTGAAGTCACAAAAGAAGGCGTGAAGCTCTGCACCATGGGTCCTGGAA
  AAGTGTTCGGGGAGCTGGCTATACTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGT
  GAAACTCTGGGCCATCGATCGACAATGTTTTCAAACAATAATGATGAGGACAGGACTCATCAAGCATACC
  GAGTACATGGAATTTTTAAAAAGTGTTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGG
  CTGATGTCCTCGAAGAGACCCACTATGAAAATGGAGAATATATCATCAGGCAGGGTGCAAGAGGAGACAC
  CTTCTTCATCATCAGTAAAGGGCAGGTGAATGTTACTCGAGAAGACTCACCAAGTGAAGACCCAGTCTTC
  CTTAGAACTTTAGGGAAGGGAGATTGGTTTGGAGAGAAAGCGTTGCAGGGGGAGGATGTGAGAACAGCAA
  ATGTTATTGCCGCAGAAGCTGTCACCTGCCTTGTGATTGACAGAGACTCTTTCAAGCATTTGATTGGAGG
  ACTGGACGATGTTTCTAACAAAGCATATGAGGACGCAGAAGCAAAAGCAAAATATGAAGCCGAAGCTGCC
  TTCTTCGCCAACCTGAAGCTGTCTGATTTCAACATCATTGACACCCTTGGAGTTGGAGGTTTCGGACGAG
  TAGAGCTGGTCCAGTTGAAAAGTGAAGAATCCAAAACATTTGCAATGAAGATCCTCAAGAAACGCCACAT
  TGTGGACACCAGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCTGACTTC
  ATTGTGAGGCTGTACAGGACATTTAAAGACAGCAAATACTTGTATATGTTGATGGAAGCGTGCCTGGGTG
  GAGAGCTCTGGACTATTCTCAGGGATAGGGGTTCGTTTGAAGACTCAACAACCAGGTTTTACACTGCATG
  TGTGGTAGAAGCATTCGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTCAAGCCGGAGAATCTC
  ATCCTAGATCATCGAGGCTATGCCAAACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGA
  AAACATGGACTTTTTGTGGGACTCCAGAATATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACAT
  TTCAGCTGACTACTGGTCACTAGGAATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGC
  CCAGATCCAATGAAAACCTACAATATCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTG
  CAAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAACCCATCAGAGAGGTTAGGAAATTTGAA
  AAACGGAGTCAAAGACATTCAGAAACACAAGTGGTTTGAGGGCTTTAATTGGGAAGGCTTAAGAAAAGGC
  ACCTTGACACCTCCCATAATTCCAAGTGTTGCGTCACCCACAGACACAAGCAATTTTGACAGTTTCCCTG
  AGGACAGCGATGAGCCACCACCTGATGACAACTCAGGCTGGGACATAGACTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013833
• Insert Size: 2016 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013833.2, NP_001013855.1
• RefSeq Size: 3914 bp
• RefSeq ORF: 2016 bp
• Locus ID: 19091
• Cytogenetics: 19 C1