Hgf (NM_010427) Mouse Untagged Clone

CAT#: MC221077

Hgf (untagged) - Mouse hepatocyte growth factor (Hgf), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hgf
• Synonyms: C230052L06Rik; HGF/SF; NK1; NK2; SF; SF/HGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221077 representing NM_010427
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTCCTGCACCTCCTCCTGCTTC
  ATGTCGCCATCCCCTATGCAGAAGGACAGAAGAAAAGAAGAAATACACTTCATGAATTTAAAAAGTCAGC
  AAAAACTACTCTTACCAAGGAAGACCCATTACTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG
  TGTGCCAACAGGTGTATCAGGAACAGGGGCTTTACGTTCACTTGCAAGGCCTTCGTTTTTGATAAGTCAA
  GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT
  TGACCTCTATGAAAACAAAGACTATATTAGAAACTGCATCATTGGTAAAGGAGGCAGCTATAAAGGGACG
  GTATCCATCACTAAGAGTGGCATCAAATGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT
  TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG
  ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT
  GAATGCATGACCTGCAATGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACTTGTC
  AGCGCTGGGACCAGCAGACACCACACCGGCACAAGTTCTTGCCAGAAAGATATCCCGACAAGGGCTTTGA
  TGATAATTATTGCCGCAATCCTGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT
  TGGGAGTATTGTGCAATTAAAACGTGCGCTCACAGTGCTGTGAATGAGACTGATGTCCCTATGGAAACAA
  CTGAATGCATTCAAGGCCAAGGAGAAGGTTACAGGGGAACCAGCAATACCATTTGGAATGGAATTCCCTG
  TCAGCGTTGGGATTCGCAGTACCCTCACAAGCATGATATCACTCCCGAGAACTTCAAATGCAAGGACCTT
  AGAGAAAATTATTGCCGCAATCCAGATGGGGCTGAATCACCATGGTGTTTTACCACTGACCCAAACATCC
  GAGTTGGCTACTGCTCTCAAATTCCCAAGTGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG
  GAAAAATTACATGGGCAACTTATCCAAAACAAGGTCTGGACTTACATGTTCCATGTGGGACAAGAATATG
  GAGGATTTACACCGTCATATCTTCTGGGAGCCAGATGCTAGCAAATTGAATAAGAATTACTGCCGGAATC
  CTGATGATGATGCCCATGGACCTTGGTGCTACACGGGGAATCCTCTTATTCCTTGGGATTATTGCCCTAT
  TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA
  ACAAAACAACTGCGGGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT
  ACAGAAATAAACATATCTGTGGAGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGACAATGTTT
  TCCAGCCAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGCATCCACGATGTTCATGAGAGAGGC
  GAGGAGAAGCGCAAGCAGATCTTAAACATTTCCCAGCTGGTCTATGGTCCTGAAGGCTCAGACTTGGTTT
  TACTGAAGCTTGCTCGACCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGTTG
  TACAATCCCTGAAAAGACCACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCGGATGGT
  TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA
  CTTTGAATGAGTCTGAGTTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCATGTGAGGGAGATTATGG
  TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT
  GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT
  TGACATACAAGTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010427
• ORF Size: 2187 bp
• Insert Size: 2187
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: BC119228, AAI19229
• RefSeq Size: 2283
• RefSeq ORF: 2187
• Locus ID: 15234
• Gene Summary: This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).