Fgd4 (NM_139232) Mouse Untagged Clone

CAT#: MC221459

Fgd4 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant alpha, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgd4
• Synonyms: 9030023J02Rik; 9330209B17Rik; Frabp; ZFYVE6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221459 representing NM_139232
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGTCTAATCCGGCCCCTACTTCTTGTACCTCAAAAGGGAAGCATAGTAAGGTATCGGATCTCA
  TCAGCCACTTTGAAGGAGGCAGTGTTTTGTCAAGTTACATTGATTTGCAGAAAGATTCTACTATGAACCT
  CAATATTCCTCAAACTCTAGGACAGCCTGGGTTAACCTCCTCACCTCCACGAAAATTTCTGCCCCAGCAC
  TCTCCACAGAAGCAGGAAAACGACCCAGATCAGACTCAGGGGCAACATGGTTGTTTGGCCAATGGTGTAG
  TGGCTGCACAAAACCAGATGGAATGTGAAGATGAGAAGGAGACTACTCTTAGCCCAGAGATGGCTATTCA
  GACTGCTGCCGCCTCTCCTGATACACATGTGCTGAATGGAGAAAGAAATGAAACCATCACAGATTCTGCA
  TCATCCATAGCCAATAGTCATGATGAAAATGCTTCTGACAGCAGCTGCAGGACTCCAGGGACGGACTTAG
  GGCTTCCCTCAAAAGAAGGGGAGCCAGGGATGGACGCTGAGCTCCAAGAGAGGGAAAATGGGGTGAACAC
  CATGGGATTGGATACGTTGGACCAGCACCATGAAGTGAAGGAGACTAATGAACAGAAACTTCACAAAATA
  GCCACTGAACTTTTACTTACAGAAAGAGCTTATGTCAGCCGGCTCGACCTCCTAGATCAGGTATTTTATT
  GCAAACTATTAGAAGAAGCAAACCGAGGCTCATTTCCTGCAGAGATGGTGAATAAAATCTTTTCTAACAT
  TTCATCAATAAATGCCTTCCATAGTAAATTCCTATTACCTGAGCTGGAGAAACGAATGCAAGAATGGGAA
  ACTACACCCAGAATTGGAGATATCCTGCAAAAGTTGGCGCCATTCCTTAAGATGTATGGAGAATACGTGA
  AGGGATTTGATAATGCAGTGGAACTGGTTAAAACCATGACAGAGCGTGTTCCCCAGTTTAAATCAGTGAC
  TGAAGAGATTCAGAAACAGAAGATCTGTGGAAGCTTAACGCTGCAGCATCACATGCTGGAGCCTATTCAG
  CGCATTCCTCGCTATGAGATGCTCCTGAAGGACTACCTGAAGAAGTTGTCTCCTGACTCCCCAGACTGGA
  ATGATGCAAAAAAGTCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGAAAAAT
  GGAGAACCTGAAGAAACTTTTAGAAATTTATGAGATGTTGGGAGAAGAAGAGGATATTGTAAATCCCTCA
  AATGAACTAATAAAAGAAGGACAAATCCTCAAACTAGCAGCTCGGAACACATCAGCACAAGAGCGCTACC
  TCTTCTTATTCAACAACATGTTGCTATATTGTGTGCCCAGATTCAGCTTGGTTGGCTCAAAATTCACAGT
  TCGAACCAGAGTTGGCATTGATGGAATGAAAATTGTGGAGACTCACAATGAAGAATATCCACACACTTTC
  CAGATATCTGGGAAAGAAAGAACCCTGGAGCTGCAGGCCAGTTCTGAACAAGACAAGGAAGAATGGATCA
  AGGCCCTTCAAGAAAGTATTGATGCTTTTCATCAAAGGCATGAAACTTTCAGAAATGCAATCGCAAAGGA
  AAATGACATTCCCCTAGAAGTTTCTACTGCTGAGCTGGGAAAACGAGCTCCAAGATGGATACGTGATAAT
  GAAGTGACCATGTGTATGAAGTGCAAAGAGTCCTTCAATGCACTGACCAGAAGGCGGCATCACTGCCGGG
  CATGTGGACATGTGGTTTGTTGGAAATGTTCTGACTACAAAGCTCAGCTTGAGTATGATGGTGGAAGATT
  GAACAAGGTTTGTAAAGACTGTTACCAGATAATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAGG
  GGCATTTTAGAGATTGAATCAGCAGAAGTATCAGGAAATAGTGAGGTGTGCAGTTTCCTTCAGTACATGG
  AAAAGTCAAAGCCTTGGCAGAAAATCTGGTGTGTGATCCCCAAGCAAGACCCCCTTGTGCTGTACATGTA
  TGGCGCTCCTCAGGATGTCAGAGCCCAAGCGACCATACCCCTCCTGGGCTACGTTGTGGATGATATGCCT
  AAGAGTGCAGATCTGCCACATAGTTTCAAGCTGACCCAGTCCAAGTCTGTGCATAGCTTTGCTGCAGACA
  ATGAGGAACTGAAACAGAAATGGCTGAAAATCATCCTTCTAGCTGTCACAGGTGAGACACCAGATGGCCC
  AAGTGAGCATCTAGCCACCTTGAACAATCTCCCTGGACCCAAGAAAAAGTCAGAATGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139232
• ORF Size: 2301 bp
• Insert Size: 2301
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_139232.3, NP_631978.1
• RefSeq Size: 8344
• RefSeq ORF: 2301
• Locus ID: 224014
• Gene Summary: This gene is a member of the FYVE, RhoGEF and PH domain containing (FGD) family. The encoded protein is a Cdc42-specific guanine nucleotide exchange factor (GEF) that plays an essential role in regulating the actin cytoskeleton and cell morphology. Disruption of the gene in mouse causes abnormal nerve development and dysmyelination. Mutations in a similar gene in human can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (alpha) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. It represents use of an alternate promoter. The resulting isoform (alpha) has a shorter N-terminus, compared to isoform 1. Variants alpha and 2 encode the same isoform (alpha).