Il4ra (NM_001008700) Mouse Untagged Clone

CAT#: MC221751

Il4ra (untagged) - Mouse interleukin 4 receptor, alpha (Il4ra), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Il4ra
• Synonyms: CD124; Il4r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221751 representing NM_001008700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGCTTTGCACCAAGTTCCTGACCTCTGTGGGCTGTCTGATTTTGCTGTTGGTGACTGGATCTG
  GGAGCATCAAGGTCCTGGGTGAGCCCACCTGCTTCTCTGACTACATCCGCACTTCCACGTGTGAGTGGTT
  CCTGGATAGCGCTGTGGACTGCAGTTCTCAGCTCTGCCTACACTACAGGCTGATGTTCTTCGAGTTCTCT
  GAAAACCTCACATGCATCCCGAGGAACAGTGCCAGCACTGTGTGTGTGTGCCACATGGAAATGAATAGGC
  CGGTCCAATCAGACAGATACCAGATGGAACTGTGGGCTGAGCACAGACAGCTGTGGCAGGGCTCCTTCAG
  CCCCAGTGGTAATGTGAAGCCCCTAGCTCCAGACAACCTCACACTCCACACCAATGTGTCCGACGAATGG
  CTGCTGACCTGGAATAACCTGTACCCATCGAACAACTTACTGTACAAAGACCTCATCTCCATGGTCAACA
  TCTCCAGAGAGGACAACCCTGCAGAATTCATAGTCTATAATGTGACCTACAAGGAACCCAGGCTGAGCTT
  CCCGATCAACATCCTGATGTCAGGGGTCTACTATACGGCGCGTGTGAGGGTCAGATCCCAGATACTCACT
  GGCACCTGGAGTGAGTGGAGTCCTAGCATCACGTGGTACAACCACTTCCAGCTGCCCCTGATACAGCGCC
  TTCCACTGGGGGTCACCATCTCCTGCCTCTGCATCCCGTTGTTTTGCCTGTTCTGTTACTTCAGCATTAC
  CAAGATTAAGAAGATATGGTGGGACCAGATTCCCACCCCAGCACGCAGTCCCTTGGTGGCCATCATCATT
  CAGGATGCACAGGTGCCCCTCTGGGATAAGCAGACCCGAAGCCAGGAGTCAACCAAGTACCCGCACTGGA
  AAACTTGTCTAGACAAGCTGCTGCCTTGCTTGCTGAAGCACAGAGTAAAGAAGAAGACAGACTTCCCGAA
  GGCTGCCCCAACCAAGTCTCTCCAGAGTCCTGGAAAGGCAGGCTGGTGTCCCATGGAGGTCAGCAGGACC
  GTCCTCTGGCCAGAGAATGTTAGTGTCAGTGTGGTGCGCTGTATGGAGCTGTTTGAGGCCCCAGTACAGA
  ATGTGGAGGAGGAAGAAGATGAGATAGTCAAAGAGGACCTGAGCATGTCACCTGAGAACAGCGGAGGCTG
  CGGCTTCCAGGAGAGCCAGGCAGACATCATGGCTCGGCTCACTGAGAACCTGTTTTCCGACTTGTTGGAG
  GCTGAGAATGGGGGCCTTGGCCAGTCAGCCTTGGCAGAGTCATGCTCCCCTCTGCCTTCAGGAAGTGGGC
  AGGCTTCTGTATCCTGGGCCTGCCTCCCCATGGGGCCCAGTGAGGAGGCCACATGCCAGGTCACAGAGCA
  GCCTTCACACCCAGGCCCTCTTTCAGGCAGCCCAGCCCAGAGTGCACCTACTCTGGCTTGCACGCAGGTC
  CCACTTGTCCTTGCAGACAATCCTGCCTACCGGAGTTTTAGTGACTGCTGTAGCCCGGCCCCAAATCCTG
  GAGAGCTGGCTCCAGAGCAGCAGCAGGCTGATCATCTGGAAGAAGAGGAGCCTCCAAGCCCGGCTGACCC
  CCATTCTTCAGGGCCACCAATGCAGCCAGTGGAGAGCTGGGAGCAGATCCTTCACATGAGTGTCCTGCAG
  CATGGGGCAGCTGCTGGCTCCACCCCAGCCCCTGCCGGTGGCTACCAGGAGTTTGTGCAGGCAGTGAAGC
  AGGGTGCCGCCCAGGATCCTGGGGTGCCTGGTGTCAGGCCTTCTGGAGACCCCGGTTACAAGGCCTTCTC
  GAGCCTGCTCAGCAGCAATGGCATCCGCGGGGACACAGCAGCAGCGGGGACTGACGATGGGCATGGAGGC
  TACAAGCCCTTCCAGAATCCTGTTCCTAACCAGTCCCCTAGCTCCGTGCCCTTATTTACTTTCGGACTAG
  ACACGGAGCTGTCACCCAGTCCTCTGAACTCAGACCCACCCAAAAGCCCCCCAGAATGCCTTGGTCTGGA
  GCTGGGGCTCAAAGGAGGTGACTGGGTGAAGGCCCCTCCTCCTGCAGATCAGGTGCCCAAGCCCTTTGGG
  GATGACCTGGGCTTTGGTATTGTGTACTCGTCCCTCACTTGCCACTTGTGTGGCCACCTGAAGCAACACC
  ACAGCCAGGAGGAAGGTGGCCAGAGCCCCATCGTTGCTAGCCCTGGCTGTGGCTGCTGCTACGATGACAG
  ATCACCATCCCTGGGGAGCCTCTCGGGGGCCTTGGAAAGCTGTCCTGAGGGAATACCACCAGAAGCCAAC
  CTCATGTCAGCACCCAAGACACCCTCAAACTTGTCAGGGGAGGGCAAGGGCCCTGGTCACTCTCCTGTTC
  CCAGCCAGACGACCGAGGTGCCTGTGGGCGCCCTGGGCATTGCTGTTTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008700
• Insert Size: 2433 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008700.3, NP_001008700.1
• RefSeq Size: 5122 bp
• RefSeq ORF: 2433 bp
• Locus ID: 16190
• Cytogenetics: 7 68.94 cM
• Gene Summary: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. [UniProtKB/Swiss-Prot Function]