Hif1a (NM_010431) Mouse Untagged Clone

CAT#: MC221924

Hif1a (untagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hif1a
• Synonyms: AA959795; bHLHe78; HIF-1-alpha; HIF1-alpha; HIF1alpha; MOP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221924 representing NM_010431
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAAGATGAGTTCTGAACGTCGAAAAGAAAAGTCTAGAG
  ATGCAGCAAGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCC
  CCACAATGTGAGCTCACATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTATTTACGTGTGAGA
  AAACTTCTGGATGCCGGTGGTCTAGACAGTGAAGATGAGATGAAGGCACAGATGGACTGTTTTTATCTGA
  AAGCCCTAGATGGCTTTGTGATGGTGCTAACAGATGACGGCGACATGGTTTACATTTCTGATAACGTGAA
  CAAATACATGGGGTTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC
  CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAAGGGAAAGAACTAAACACAC
  AGCGGAGCTTTTTTCTCAGAATGAAGTGCACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTCAGC
  AACGTGGAAGGTGCTTCACTGCACGGGCCATATTCATGTCTATGATACCAACAGTAACCAACCTCAGTGT
  GGGTACAAGAAACCACCCATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCGTCAAATATTG
  AAATTCCTTTAGATAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGATGA
  AAGAATTACTGAGTTGATGGGTTATGAGCCGGAAGAACTTTTGGGCCGCTCAATTTATGAATATTATCAT
  GCTTTGGATTCTGATCATCTGACCAAAACTCACCATGATATGTTTACTAAAGGACAAGTCACCACAGGAC
  AGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAATAC
  GAAGAACTCCCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGACTTG
  ATTTTCTCCCTTCAACAAACAGAATCTGTGCTCAAACCAGTTGAATCTTCAGATAAGAAGATGACTCAGC
  TGTTCACCAAAGTTGAATCAGAGGCTACAAGCTGCCTTTTTGATAAGCTTAAGAAGGAGCCTGATGCTCT
  CACTCTGCTGGCTCCAGCTGCCGGCGACACCATCATCTCTCTGGATTTTGGCAGCGATGACACAGAAACT
  GAAGATCAACAACTTGAAGATGTTCCATTATATAATGATGTAATGTTTCCCTCTTCTAATGAAAAATTAA
  ATATAAACCTGGCAATGTCTCCTTTACCTTCATCGGAAACTCCAAAGCCACTTCGAAGTAGTGCTGATCC
  TGCACTGAATCAAGAGGTTGCATTAAAATTAGAATCAAGTCCAGAGTCACTGGGACTTTCTTTTACCATG
  CCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGTTCACCTGAGAGACTTC
  TTCAGGAAAACGTAAACACTCCTAACTTTTCCCAGCCTAACAGTCCCAGTGAATATTGCTTTGATGTGGA
  TAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGAAAAACTGTTTGCTGAAGACACAGAGGCAAAG
  AATCCATTTTCAACTCAGGACACTGATTTAGACTTGGAGATGCTGGCTCCCTATATCCCAATGGATGATG
  ATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAGAGAGCAATTCTCCAAGCCCTCCAAGTATGAG
  CACAGTTACTGGGTTCCAGCAGACCCAGTTACAGAAACCTACCATCACTGCCACTGCCACCACAACTGCC
  ACCACTGATGAATCAAAAACAGAGACGAAGGACAATAAAGAAGATATTAAAATACTGATTGCATCTCCAT
  CTTCTACCCAAGTACCTCAAGAAACGACCACTGCTAAGGCATCAGCATACAGTGGCACTCACAGTCGGAC
  AGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAACAGACAGACAAAGCTCATCCAAGGAGCCTTAAC
  CTGTCTGCCACTTTGAATCAAAGAAATACTGTTCCTGAGGAAGAATTAAACCCAAAGACAATAGCTTCGC
  AGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCTCCCTTTTTCAAGCAGCAGGAATTGGAACATT
  ATTGCAGCAACCGGGTGACTGTGCACCTACTATGTCACTTTCCTGGAAACGAGTGAAAGGATTCATATCT
  AGTGAACAGAATGGAACGGAGCAAAAGACTATTATTTTAATACCCTCCGATTTAGCATGCAGACTGCTGG
  GGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCAGTTACGATTGTGAAGTTAATGCTCCCATACA
  AGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010431
• ORF Size: 2511 bp
• Insert Size: 2511
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: BC026139, AAH26139
• RefSeq Size: 4775
• RefSeq ORF: 2511
• Locus ID: 15251
• Gene Summary: This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.