Ube3a (NM_001033962) Mouse Untagged Clone

CAT#: MC222043

Ube3a (untagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube3a
• Synonyms: 4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a; mKIAA4216
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222043 representing NM_001033962
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATG
  AGGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTAT
  TAAAGCCCTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGC
  TCAGCTTACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGGAA
  AAGATTTTAAAGATGTGATTTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGAGAGAG
  TGAGGATTATTCCCCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCTGAGC
  TTTCGGAAAGTCAAACAGCACACAAAGGAGGAATTGAAATCTCTTCAAGAAAAGGATGAAGACAAGGATG
  AAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAG
  GATGGGTGATAGTTCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATT
  GATGCTATTAGAAGGGTCTACAGCAGTTTGCTCGCTAATGAAAAATTAGAAACTGCCTTCCTGAATGCAC
  TTGTATATCTGTCACCTAACGTGGAATGTGATTTGACATATCATAATGTGTATACTCGAGATCCTAATTA
  TCTCAATTTGTTCATTATTGTAATGGAGAATAGTAATCTCCACAGTCCTGAATATCTGGAAATGGCGTTG
  CCATTATTTTGCAAAGCTATGTGTAAGCTACCCCTTGAAGCTCAAGGAAAACTGATTAGGCTGTGGTCTA
  AATACAGTGCTGACCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAG
  CAATGAATTTAATAGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAA
  ATGGTTTACTATGCAAATGTAGTGGGAGGGGATGTGGACACAAATCATAATGAGGAAGATGATGAAGAAC
  CCATACCTGAGTCCAGCGAATTAACACTTCAGGAGCTTCTGGGAGATGAAAGAAGAAATAAGAAAGGTCC
  TCGAGTGGATCCACTAGAAACCGAACTTGGCGTTAAAACTCTAGACTGTCGAAAACCACTTATCTCCTTT
  GAAGAATTCATTAATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACCTTTTTCAAAGTTG
  AAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATT
  ATATTATGACAATAGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGA
  CAGCAGTTGAATCCGTATTTGAGACTCAAAGTCAGACGTGACCATATTATAGATGATGCACTGGTCCGGC
  TAGAGATGATTGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACA
  AGGAGTAGATGAGGGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATTTTTAATCCAGAT
  ATTGGTATGTTCACATATGATGAAGCTACGAAATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGG
  GTCAGTTTACTCTGATTGGCATAGTCCTGGGTCTGGCTATTTACAATAATTGTATACTGGATGTCCATTT
  TCCCATGGTTGTATACAGGAAGCTAATGGGGAAAAAAGGAACCTTTCGTGACTTGGGAGACTCTCACCCA
  GTTTTATATCAGAGTTTAAAGGATTTATTGGAATATGAAGGGAGTGTGGAAGATGATATGATGATCACTT
  TCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAAT
  TCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTCTATTCAGACTACATTCTCAATAAATCTGTA
  GAAAAACAATTCAAGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCGCCCTTAAAATACTTAT
  TCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAGGCACTAGAAGAAAC
  TACAGAGTATGACGGTGGCTATACGAGGGAATCTGTTGTGATTAGGGAGTTCTGGGAAATTGTTCATTCG
  TTTACAGATGAACAGAAAAGACTCTTTCTGCAGTTTACAACAGGCACAGACAGAGCACCTGTTGGAGGAC
  TAGGAAAATTGAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTG
  CTTTAATGTCCTTTTACTTCCGGAATATTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATC
  ACATATGCCAAAGGATTTGGCATGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033962
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033962.1, NP_001029134.1
• RefSeq Size: 4910 bp
• RefSeq ORF: 2550 bp
• Locus ID: 22215
• Cytogenetics: 7 33.95 cM