Whrn (NM_001008792) Mouse Untagged Clone

CAT#: MC222532

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; C430046P22Rik; Dfnb31; Ush2d; wi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222532 representing NM_001008792
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCACAGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCGGCGG
  CGGCGGGCGGCGGCGGGGGCGCGGGGTTGCGGCTGCTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCT
  CACGGCGCTGCTGAGCGAGCCCGAGCGGGAGCAGTTCACTCACTGCCTCAACGCGTACCACGCGCGCCGC
  AACGTCTTCGACCTGGTGCGCACCCTGCGCGTCCTGCTGGACAGCCCGGTCAAGCGGCGTCTGCTGCCCA
  TGCTGCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAGTACACGGCCGAGGGCCTCTACCT
  GCCAGCCACCACCCCCTACAGGCAGCCCGCCTGGGCCGCTCCCGACGGCGCGGGGCCCGGGGAGGTGCGA
  CTCGTGAGCCTGCGGCGCGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGCGGGGGCTCGGAACACG
  GCGTGGGCATCTACGTGTCTCTAGTGGAGCCGGGCTCCCTGGCAGAGAAGGAAGGGTTGCGGGTCGGGGA
  CCAGATTCTGCGCGTCAACGATAAATCTCTAGCCCGGGTGACCCACGCGGAGGCTGTCAAGGCTCTCAAA
  GGCTCCAAGAAGCTGGTGCTGTCTGTATACTCAGCTGGGCGTATCCCAGGGGGCTATGTGACCAACCACA
  TCTACACCTGGGTGGACCCACAGGGTCGAAGCACATCCCCTCCCTCCAGCCTGCCCCAGCCCCATGGCAG
  CACCCTGAGACAGCGTGAAGATGACCGAAGGAGTACCCTCCACCTCCTGCAGAGTGGAGATGAGAAAAAG
  GTGAACCTGGTGTTGGGGGACGGCCGGTCCTTGGGCCTCACGATCCGAGGTGGAGCAGAGTACGGCCTTG
  GCATTTACATCACTGGTGTGGACCCAGGCTCTGAAGCAGAGAGCAGCGGCCTCAAGGTTGGAGACCAGAT
  TCTGGAGGTGAATGGGCGGAGCTTTCTCAACATCCTGCATGATGAGGCAGTGAAGCTGCTCAAGTCATCC
  CGGCACCTCATCCTGACGGTGAAGGACGTCGGAAGGCTGCCCCACGCACGTACCACCGTGGACCAGACCA
  AGTGGATCGCCAGTTCCCGGATCGGGGAAAGCGTCGCCAACTCAGCAGGGTCTGGTCTCAGGTTTCCAGG
  GGACCACACAGAAGAAGGGACAAGCAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACCCTG
  AGCAGCCTGGGGAATCAGACTCGTGCACTGCTGGACGACCAAGCCAGGCACCTGCTGACAGAGCAGGAGC
  GCGCCACCATGATGTACTACCTGGCCCAGTACCGTGGTGGCACCATCTCTGTGGAGGCCATGGTCATGGC
  ACTGTTTGAGTTGCTCAACACACACGCCAAGTTCTCACTCCTGTCTGAGGTGAGAAGCATCATCTCACCC
  CAAGACCTGGACCGCTTTGACCACCTGGTGCTGAGGCGCGAGATCGAGTCTATGAAGGCGAGGCAGCCCC
  CCGGCCCCGGGGTTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAGGCTCATCCACAGGCAGCCA
  CGGCACCTCTACAACCGTCAGCTCGGCCAGGAATACATTAGACCTGGAAGGAACCGGTGAGACCACCCAG
  GGCAGCACCAACGCTCTCCCGGATGTGTCTGTGGATGATGTCAAATCCCCCTCAGAAGATCTGCCTGGTA
  TCAAGCCACCTCCTCCCCCACCACCCCTGGCTCAAGGTCATGACCGCTTGCTTGGCCAGCCACGGAAGCC
  AGGGAGGGAGGACCCCGCACCTCTGTCTTCTGCTGCCCACTCAGGCATTGTCTTCTCAGCACCACGGAAC
  CGCAGCCCGCCGCCAGGGACTGCTCCCACTCCGGGACCTTCCTCAGCACAGGACTCTCCCTCCTCCCCCA
  TTTATGCCTCCATCTCCCATGCCAACCCCAGTTCCAGAAAGCCGCTGGACACCCACCTGGCCCTGGTTAA
  CCAGCACCCCATCGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCACCTGAAGAGCCCTCCTGCAGAGACC
  CCAGGAGCTGGGGCCTGCCTTCCACCACCATCACCCTCTGAACACCCTGACGCCGTGGGTGCAAACCAGC
  ACTTTGTTCTGGTGGAGGTGCACCGTCCGGACAGTGAGCCTGATGTGAATGAAGTGCGGGCTCTGCCCCA
  GACTCGCACAGCCTCCACACTCTCTCAGCTCTCAGACAGTGGGCAGACCCTGAGCGAGGACAGTGGCGTG
  GATGCCGGGGAGACGGAGGCCAGCACCTCAGGCCGAGGCAGACAGACAGCATCCGCTAAGAACAAGAATG
  GCAAGGAGCAGCCCCGGACGGAGAGGACCGCGGAGGGTGCCAACAAACCTCCCGGCCTGCTGGAGCCAAC
  GTCCACCCTGGTCCGTGTGAGGAAAAGTGCAGCCACACTGGGCATCGCCATTGAGGGCGGTGCCAACACA
  CGCCAGCCTCTGCCCAGGATCGTCACAATTCAGCGAGGAGGTTCTGCCCATAACTGTGGACAGCTCAAGG
  TGGGCCACGTAATTCTGGAAGTGAATGGGCAGACACTTCGGGGTAAGGAGCACAAGGAGGCCGCCCGAAT
  CATCGCTGAGGCCTTCAAGACCAAGGAGAGAGACTACATCGACTTTCTGGTCACTGAGTTCAACGTGATG
  CTCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001008792
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008792.2, NP_001008792.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 2736 bp
• Locus ID: 73750
• Cytogenetics: 4 C1
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks an in-frame exon in the coding region, compared to variant 2. The encoded isoform (3) is shorter than isoform 2.