Whrn (NM_001008791) Mouse Untagged Clone

CAT#: MC222594

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; C430046P22Rik; Dfnb31; Ush2d; wi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222594 representing NM_001008791
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCACAGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCGGCGG
  CGGCGGGCGGCGGCGGGGGCGCGGGGTTGCGGCTGCTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCT
  CACGGCGCTGCTGAGCGAGCCCGAGCGGGAGCAGTTCACTCACTGCCTCAACGCGTACCACGCGCGCCGC
  AACGTCTTCGACCTGGTGCGCACCCTGCGCGTCCTGCTGGACAGCCCGGTCAAGCGGCGTCTGCTGCCCA
  TGCTGCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAGTACACGGCCGAGGGCCTCTACCT
  GCCAGCCACCACCCCCTACAGGCAGCCCGCCTGGGCCGCTCCCGACGGCGCGGGGCCCGGGGAGGTGCGA
  CTCGTGAGCCTGCGGCGCGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGCGGGGGCTCGGAACACG
  GCGTGGGCATCTACGTGTCTCTAGTGGAGCCGGGCTCCCTGGCAGAGAAGGAAGGGTTGCGGGTCGGGGA
  CCAGATTCTGCGCGTCAACGATAAATCTCTAGCCCGGGTGACCCACGCGGAGGCTGTCAAGGCTCTCAAA
  GGCTCCAAGAAGCTGGTGCTGTCTGTATACTCAGCTGGGCGTATCCCAGGGGGCTATGTGACCAACCACA
  TCTACACCTGGGTGGACCCACAGGGTCGAAGCACATCCCCTCCCTCCAGCCTGCCCCAGCCCCATGGCAG
  CACCCTGAGACAGCGTGAAGATGACCGAAGGAGTACCCTCCACCTCCTGCAGAGTGGAGATGAGAAAAAG
  GTGAACCTGGTGTTGGGGGACGGCCGGTCCTTGGGCCTCACGATCCGAGGTGGAGCAGAGTACGGCCTTG
  GCATTTACATCACTGGTGTGGACCCAGGCTCTGAAGCAGAGAGCAGCGGCCTCAAGGTTGGAGACCAGAT
  TCTGGAGGTGAATGGGCGGAGCTTTCTCAACATCCTGCATGATGAGGCAGTGAAGCTGCTCAAGTCATCC
  CGGCACCTCATCCTGACGGTGAAGGACGTCGGAAGGCTGCCCCACGCACGTACCACCGTGGACCAGACCA
  AGTGGATCGCCAGTTCCCGGATCGGGGAAAGCGTCGCCAACTCAGCAGGGTTTCCAGGGGACCACACAGA
  AGAAGGGACAAGCAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACCCTGAGCAGCCTGGGG
  AATCAGACTCGTGCACTGCTGGACGACCAAGCCAGGCACCTGCTGACAGAGCAGGAGCGCGCCACCATGA
  TGTACTACCTGGCCCAGTACCGTGGTGGCACCATCTCTGTGGAGGCCATGGTCATGGCACTGTTTGAGTT
  GCTCAACACACACGCCAAGTTCTCACTCCTGTCTGAGGTGAGAAGCATCATCTCACCCCAAGACCTGGAC
  CGCTTTGACCACCTGGTGCTGAGGCGCGAGATCGAGTCTATGAAGGCGAGGCAGCCCCCCGGCCCCGGGG
  TTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAGGCTCATCCACAGGCAGCCACGGCACCTCTAC
  AACCGTCAGCTCGGCCAGGGAGCGGCTGCTGTGGCTTATAGACCTGATGGAGAATACATTAGACCTGGAA
  GGAACCGGTGAGACCACCCAGGGCAGCACCAACGCTCTCCCGGATGTGTCTGTGGATGATGTCAAATCCC
  CCTCAGAAGATCTGCCTGGTATCAAGCCACCTCCTCCCCCACCACCCCTGGCTCAAGGTCATGACCGCTT
  GCTTGGCCAGCCACGGAAGCCAGGGAGGGAGGACCCCGCACCTCTGTCTTCTGCTGCCCACTCAGGCATT
  GTCTTCTCAGCACCACGGAACCGCAGCCCGCCGCCAGGGACTGCTCCCACTCCGGGACCTTCCTCAGCAC
  AGGACTCTCCCTCCTCCCCCATTTATGCCTCCATCTCCCATGCCAACCCCAGTTCCAGAAAGCCGCTGGA
  CACCCACCTGGCCCTGGTTAACCAGCACCCCATCGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCACCTG
  AAGAGCCCTCCTGCAGAGACCCCAGGAGCTGGGGCCTGCCTTCCACCACCATCACCCTCTGAACACCCTG
  ACGCCGTGGGTGCAAACCAGCACTTTGTTCTGGTGGAGGTGCACCGTCCGGACAGTGAGCCTGATGTGAA
  TGAAGTGCGGGCTCTGCCCCAGACTCGCACAGCCTCCACACTCTCTCAGCTCTCAGACAGTGGGCAGACC
  CTGAGCGAGGACAGTGGCGTGGATGCCGGGGAGACGGAGGCCAGCACCTCAGGCCGAGGCAGACAGACAG
  CATCCGCTAAGAACAAGAATGGCAAGGAGCAGCCCCGGACGGAGAGGACCGCGGAGGGTGCCAACAAACC
  TCCCGGCCTGCTGGAGCCAACGTCCACCCTGGTCCGTGTGAGGAAAAGTGCAGCCACACTGGGCATCGCC
  ATTGAGGGCGGTGCCAACACACGCCAGCCTCTGCCCAGGATCGTCACAATTCAGCGAGGAGGTTCTGCCC
  ATAACTGTGGACAGCTCAAGGTGGGCCACGTAATTCTGGAAGTGAATGGGCAGACACTTCGGGGTAAGGA
  GCACAAGGAGGCCGCCCGAATCATCGCTGAGGCCTTCAAGACCAAGGAGAGAGACTACATCGACTTTCTG
  GTCACTGAGTTCAACGTGATGCTCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001008791
• Insert Size: 2757 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008791.2, NP_001008791.1
• RefSeq Size: 4089 bp
• RefSeq ORF: 2757 bp
• Locus ID: 73750
• Cytogenetics: 4 C1
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).