Erbb2 (NM_001003817) Mouse Untagged Clone

CAT#: MC224084

Erbb2 (untagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erbb2
• Synonyms: c-erbB2; c-neu; Erbb-2; HER-2; HER2; l11Jus8; mKIAA3023; Neu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224084 representing NM_001003817
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGCGGCCTGGTGCCGTTGGGGGTTCCTCCTCGCCCTCCTGTCCCCCGGAGCCGCGGGTACCC
  AAGTGTGTACCGGTACCGACATGAAGTTGCGACTCCCTGCCAGTCCTGAGACCCACCTGGACATGCTTCG
  CCACCTCTACCAGGGCTGTCAGGTGGTGCAGGGCAATTTGGAGCTTACCTACCTGCCCGCCAATGCCAGC
  CTCTCATTCCTGCAGGACATCCAGGAAGTCCAGGGATACATGCTCATCGCTCACAACCGAGTGAAACACG
  TCCCACTGCAGAGGTTGCGCATCGTGAGAGGGACTCAGCTCTTTGAGGACAAGTATGCCCTGGCTGTGCT
  AGACAACCGAGACCCTTTGGACAACGTCACCACCGCCGCCCCAGGCAGAACCCCAGAAGGGCTGCGGGAG
  CTGCAGCTTCGAAGTCTCACAGAGATCTTGAAGGGAGGAGTTTTGATCCGTGGGAACCCTCAGCTCTGCT
  ACCAGGACATGGTTTTGTGGAAGGATGTCCTCCGTAAGAATAACCAGCTGGCTCCTGTCGACATGGACAC
  CAATCGTTCCCGGGCCTGTCCACCTTGTGCCCCAACCTGCAAAGACAATCACTGTTGGGGTGAGAGTCCT
  GAAGACTGTCAGATCTTGACTGGCACCATCTGTACTAGTGGCTGTGCCCGGTGCAAGGGCCGGCTGCCCA
  CTGACTGTTGCCATGAGCAGTGTGCTGCAGGCTGCACGGGTCCCAAGCATTCTGACTGCCTGGCCTGCCT
  CCACTTCAATCATAGTGGTATCTGTGAGCTGCACTGCCCGGCCCTCATCACCTACAACACAGACACCTTC
  GAGTCCATGCTCAACCCTGAGGGTCGCTACACCTTTGGTGCCAGCTGTGTGACCACCTGCCCCTACAACT
  ACCTCTCCACGGAAGTGGGATCCTGCACTCTGGTCTGTCCCCCGAACAACCAAGAGGTCACAGCTGAGGA
  CGGAACACAGCGGTGTGAGAAATGCAGCAAGCCCTGTGCTGGAGTATGCTATGGTCTGGGCATGGAGCAC
  CTCCGAGGGGCGAGGGCCATCACCAGTGACAATATCCAGGAGTTTGCTGGCTGCAAGAAGATCTTTGGGA
  GCCTGGCATTTTTGCCGGAGAGCTTTGATGGGAACCCCTCCTCCGGCGTTGCCCCACTGAAGCCAGAGCA
  TCTCCAAGTGTTCGAAACCCTGGAGGAGATCACAGGTTACCTATACATTTCAGCATGGCCAGAGAGCTTC
  CAAGACCTCAGTGTCTTCCAGAACCTTCGGGTCATTCGGGGACGGATTCTCCATGATGGTGCTTACTCAT
  TGACGTTGCAAGGCCTGGGGATTCACTCACTGGGGCTACGCTCACTGCGGGAGCTGGGCAGTGGATTGGC
  TCTCATTCACCGCAACACCCATCTCTGCTTTGTAAACACTGTACCTTGGGACCAGCTCTTCCGGAACCCG
  CACCAGGCCCTACTCCACAGTGGGAACCGGCCAGAAGAGGCATGTGGTCTTGAGGGCTTGGTCTGTAACT
  CACTGTGTGCCCGTGGGCACTGCTGGGGGCCAGGGCCCACCCAGTGTGTCAACTGCAGTCAGTTCCTCCG
  GGGCCAGGAGTGTGTGGAGGAGTGCCGAGTATGGAAGGGGCTCCCCAGGGAGTATGTGAGGGGCAAGCAC
  TGTCTGCCATGCCACCCCGAGTGTCAGCCTCAAAACAGCTCGGAGACCTGCTATGGATCGGAGGCTGACC
  AGTGTGAGGCTTGTGCCCACTACAAGGACTCATCTTCCTGTGTGGCTCGCTGCCCCAGTGGTGTGAAGCC
  AGACCTCTCCTACATGCCTATCTGGAAGTACCCGGATGAGGAGGGCATATGTCAGCCATGCCCCATCAAC
  TGCACCCACTCATGTGTGGACCTGGACGAACGAGGCTGCCCAGCAGAGCAGAGAGCCAGCCCAGTGACAT
  TCATCATTGCAACTGTGGTGGGCGTCCTGTTGTTCCTGATCATAGTGGTGGTCATTGGAATCCTAATCAA
  ACGAAGGCGACAGAAGATCCGGAAGTATACCATGCGTAGGCTGCTGCAGGAGACCGAGCTGGTGGAGCCG
  CTGACGCCCAGTGGAGCTGTGCCCAACCAGGCTCAGATGCGGATCCTAAAGGAGACAGAGCTAAGGAAGC
  TGAAGGTGCTTGGGTCAGGAGCCTTCGGCACTGTCTACAAGGGCATCTGGATCCCAGATGGGGAGAACGT
  GAAAATCCCCGTGGCCATCAAGGTGTTGAGGGAAAACACATCTCCTAAAGCTAACAAAGAAATCCTAGAT
  GAAGCGTACGTCATGGCTGGTGTGGGTTCTCCATATGTGTCCCGCCTCCTGGGCATCTGCCTGACATCCA
  CAGTGCAGCTGGTGACACAGCTTATGCCCTATGGCTGCCTTCTGGACCATGTCCGAGAACACCGAGGTCG
  CTTAGGCTCCCAGGACCTGCTCAACTGGTGTGTTCAGATTGCCAAGGGGATGAGCTACCTGGAGGAAGTT
  CGGCTTGTTCACAGGGACCTAGCTGCCCGAAACGTGCTAGTCAAGAGTCCCAACCACGTCAAGATTACCG
  ACTTCGGGCTGGCACGGCTGCTGGACATTGATGAGACTGAATACCATGCAGATGGGGGCAAGGTGCCCAT
  CAAGTGGATGGCATTGGAATCTATTCTCAGACGCCGGTTCACCCATCAGAGTGATGTGTGGAGCTATGGT
  GTGACTGTGTGGGAGCTGATGACCTTTGGGGCCAAACCTTACGATGGGATCCCAGCTCGGGAGATCCCTG
  ATTTGCTGGAGAAGGGAGAACGCCTACCTCAGCCTCCAATCTGCACCATCGACGTCTACATGATCATGGT
  CAAATGTTGGATGATTGACTCCGAATGTCGCCCGAGATTCCGGGAGTTGGTATCAGAATTCTCCCGTATG
  GCAAGGGACCCCCAGCGCTTTGTGGTCATCCAGAACGAGGACTTAGGCCCCTCCAGCCCCATGGACAGCA
  CCTTCTACCGTTCACTGCTGGAGGATGATGACATGGGGGAGCTGGTCGATGCTGAAGAGTACCTGGTACC
  CCAGCAGGGATTCTTCTCCCCAGACCCTGCCCTAGGTACTGGGAGCACAGCCCACCGCAGACACCGCAGC
  TCGTCGGCCAGGAGTGGCGGTGGTGAGCTGACACTGGGCCTGGAGCCCTCGGAAGAAGAGCCCCCCAGAT
  CTCCACTGGCTCCCTCCGAAGGGGCTGGCTCCGATGTGTTTGATGGTGACCTGGCAGTGGGGGTAACCAA
  AGGACTGCAGAGCCTCTCTCCACATGACCTCAGCCCTCTACAGCGGTACAGTGAGGATCCCACATTACCT
  CTGCCCCCCGAGACTGATGGCTACGTTGCTCCCCTGGCCTGCAGCCCCCAGCCCGAGTATGTGAACCAGC
  CAGAGGTTCGGCCTCAGTCTCCCTTGACCCCAGAGGGTCCTCCGCCTCCCATCCGACCTGCTGGTGCTAC
  TCTAGAAAGACCCAAGACTCTCTCTCCTGGGAAAAATGGGGTTGTCAAAGACGTTTTTGCCTTTGGGGGT
  GCTGTGGAGAACCCTGAATACTTAGCACCCAGAGCAGGCACTGCCTCTCAGCCCCACCCTTCTCCTGCCT
  TCAGCCCAGCCTTTGACAACCTCTATTACTGGGACCAGAACTCATCGGAGCAGGGTCCTCCACCAAGTAC
  CTTTGAAGGGACCCCCACTGCAGAGAACCCTGAGTACCTAGGCCTGGATGTGCCAGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003817
• Insert Size: 3771 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003817.1, NP_001003817.1
• RefSeq Size: 4998 bp
• RefSeq ORF: 3771 bp
• Locus ID: 13866
• Cytogenetics: 11 D