Depdc5 (NM_001025426) Mouse Untagged Clone

CAT#: MC224652

Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Depdc5
• Synonyms: AV016528
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224652 representing NM_001025426
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACCACAAAGGTCTACAAACTTGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG
  TCGTGAACCCTAAAGTATTTCCTCACATCAAACTTGGAGACATTGTGGAGATTGCTCACCCCAATGATGA
  GTACAGTCCTTTGCTTTTGCAAGTCAAGTCGCTTAAGGAAGATTTACAGAAAGAAACTATCAGTGTGGAC
  CAGACTGTGACTCAAGTATTCCGGCTAAGACCTTATCAAGATGTCTATGTGAATGTTGTAGACCCCAAGG
  ATGTGACTCTTGACCTGGTGGAATTGACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT
  AAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACTCAAAAAGTGGAATTTGCTGGCATCAGGGCACAG
  GCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGACCAGGGTGGTGT
  TCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA
  TGGGGATCTGTACTTTGAGAAAGCTGTGAACGGTTTCCTCGCCGACCTGTTTACTAAATGGAAGGAGAAG
  AACTGTAGTCATGAAGTGACTGTGGTCCTGTTTTCCAGAACTTTCTATGATGCAAAATCTATTGATGAAT
  TTCCTGAAATAAACCGAGCTTCAATTCAAGAGGATCACAAAGGGAGATTCTATGAGGACTTTTACAAAGT
  GGTGGTGCAGAATGAGAGAAGGGAAGAGTGGACTTCACTCCTCGTGACCATTAAAAAACTCTTCATCCAG
  TATCCAGTGTTGGTGCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTACCTCAGCACAAG
  GAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAACCGAAACTTTGA
  CCGAACTGGGCAGATGTCTGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG
  ATCTTGACCAAGCAGCGGATGATTGATAATGGAATTGGTGTGGACTTAGTGTGCATGGGAGAGCAGCCAC
  TACATGCTGTGCCATTATTCAAGCTACATAACCGGAGTGTTCCGAGGGATTCTCGACTGGGTGATGATTA
  TAATATTCCTCACTGGATAAACCATAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAACAGTTTCACT
  CCACGGATAAAACTAGCAGGAAAGAAGTCTGCCTCTGAGAAAACCAAAAATGGTCGTGATACATCTCTCG
  GGACTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGATGCTCAAGTGTT
  CAGGCTGCCTGGCCCATCCCGGGCTCAGCGCCTCGCCACCTGCAGGTCTGTGAGAGAACAGGAGAATCAC
  AGTCGCAAAAGTGCAAGCTCCTGTGATGTCTCATCCAGCCCTTCCCTGCCAAGCCGTGCACTGCCCACTG
  AGGAAGTGAGGAGCCAGGCTTCTGATGACAGCTCCCTGGGCAAGAGCACCAACATCCTGATGATCCCTAA
  CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATGTCCTGGAGAAC
  ATGATAGAACCCCCACAGAGGGACTCCAGTGCACCAGGAAGGTTCCATGTGGGTAGTGCAGAGTCCATGC
  TCCATGTCCGACCTGGAGGATACACGCCTCAGAGAGCGCTGATTAACCCCTTCGCCCCCTCGAGGATGCC
  CATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTAGGCCCATCTGGAGAGGCCATC
  CAGATCCATCATCAGACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGAGGGACCCTACCCACT
  CCTCTGCAGAGCTGTTGGAGTTGGCATACCATGAAGCTGCCGGGAGGCACAGCACTTCCCGCCAGCCTGG
  TGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCAACAGCAGTACA
  GGTGTGAATCCTAGGACCCAGAACAAGGACTCCCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAATTC
  TGACACTGTCTGCTCCCCCTGTAGTGCCAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTAC
  TACTCCTGCATGCCTTCCCCTCACCACTGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACA
  GAGGGCTGCTATGATCTCCTCCCAGAAGCAGACATGGACAGGAGGGATGAGGAGGGTGTTCAAATGACAG
  CCCAGCAAGTGTTTGAAGAGTTCATCTGCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAA
  GACCCAGAAACCCAACACCACAGTCCCACCCCCATTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTG
  TCCCGAAATCGCCCTGAGGAGGAAGGCCAGTATTGGTTAAGTATGGGCAGAACTTTCCATAAGGTGACAC
  TCAAGGACAAGATGATCACAGTAACTCGTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTA
  CACCTACAGCCTCTGCCCTTCCCACTCTGACTCGGAGTTTGTCTCCTGTTGGGTGGATTTCTGCCACGAA
  CGGCTCGAGGAATACAAGTGGAATTACTTAGATCAGTATATTTGTTCTGCTGGCTCTGAAGACTTCAGCT
  TAATCGAGTCTCTGAAGTTCTGGAGAACCCGATTTTTACTACTGCCAGCCTGTGTTACTGCCACCAAGCG
  CATCACAGAAGGGGAGGTGCACTGTGACATCTATGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAG
  CTTCTAGATGGCTTTATTCGCTTTGTAGAGGGCTTAAATCGCATCCGCAGGCGCCACCGCTCAGACCGCA
  TGATTCGGAAGGGGACTGCCATGAAAGGCTTGCAGATGACCGGGCCCATCTCTGCACACTCTCTCGAGGC
  AGCAGGCCCTCCAGTCGGCAAGAAAGGAACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAAGCTAGTCAG
  AAGAGCCTAGGAGAACAGCAGACGACTGTGCACGGAAAAAGCTCCACTCAGCCAGCTGAGAACAGCAGTG
  TTGCCATGACTCCCACCTATGTGGACAGCCCACGCAAGGACGGGGCCTTCTTTATGGAGTTTGTCCGCAG
  CCCACGCACAGCATCATCTGCCTTCTACCCTCAGGCATCTGTGGACCAAACAGCTCCTCTGGTGTTAGAC
  AGCACCAGTTTAGGGGTCAGCACAGGCCAGCCCATGGATAGAGGCAACAACCAGACCTTTGGGAACTCCC
  AGAACATAGAACAGGCCTTTCCCTCTGCAAACTCTGGTGACTACAGCTCTCAGCAGCATGTAGCAAGCTC
  TCTAACATCGTCTTCTACCCTGGTGGAGATTCTGGAAGCCATGAAGCATCCCTCGACAGGAGTCCAGCTG
  CTGTCTGAACAGAAGGGCCTTTCACCGTGCTGCTTCATCAGTGCTGAGGTCGTACACTGGTTGATGAACA
  ACGTGGAGGGGGTCCAGACGCAGGCGATGGGCATAGACATCATGCAGAAAATGCTGGAAGAGCAGCTCAT
  AACGCATGCATCTGGTGAAGCATGGCGGACCTTCATCTATGGCTTCTATTTCTACAAGATAGTAATGGAC
  AAAGAACCGGAAAGAGTGGCTATGCAGCAGCCCTCTGCCCCCTGGTACACAGCAGGAGCGGATGACTTTG
  CCAGCTTCCAACGCAAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTTGTGCACTCTGAGATTCCTGC
  CTTCCTCCTGCCCTGGCTACCTAGCCGGCCAGCCTCTTATGCAAGCAGACACAGCTCCTTTAGCCGAAGT
  TTTGGAGGACGGAGCCAGGCAGCTGCGCTGTTAGCTGCTACTGTCCCAGAGCAGAGGACTGTGACCCTGG
  ATGTTGATGTGAACAACCGAACGGACCGGCTGGAATGGTGCAGCTGTTACTACCATGGAAACTTCTCTCT
  CAATGCCGCCTTTGAGATCAAGCTACACTGGATGGCAGTGACTGCCACAGTGCTGTTTGAGATGGTCCAA
  GGTTGGCATCGAAAAGCTACCTCCTGTGGCTTCTTGCTGGTCCCAGTTTTGGAGGGTCCTTTTGCTCTGC
  CCAGTTACCTGTATGGTGATCCCCTGAGGGCCCAGCTCTTCATCCCGCTCAACCTCAGCTGCTTGCTCAA
  GGAGGGCAGCGAGCACCTGTTCGATAGTTTGGATTTGTGCAAGATAAATATTCTGTCTCCGCTTTTAACT
  TCCCTGCTGAGAATAAGCCACAGTATATCCATGTCACAGGAACGGTGTTCCTTCAGCTGCCGTACTCCAA
  ACGCAAGTTTTCAGGGCAGCAGCGGCGGCGACGGAACTCTACCAGCTCCACCAACCAAAACATGTTCTGT
  GAGGAGCGGGTTGGCTACAACTGGGCCTACAACACCATGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025426
• Insert Size: 4593 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025426.2, NP_001020597.1
• RefSeq Size: 8213 bp
• RefSeq ORF: 4593 bp
• Locus ID: 277854
• Cytogenetics: 5 17.35 cM