March8 (NM_001302385) Mouse Untagged Clone

CAT#: MC226547

42071 (untagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: March8
• Synonyms: 1300017E09Rik; MARCH-VIII; Mir
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226547 representing NM_001302385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCATCCAAGCAACATTTCTAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTGCCT
  TCTCTCGCACTTCTGTCACACCATCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGACGA
  GAGCCCTCTGATCACCCCCTGTCACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAGCAG
  TGGATCAAGAGTTCTGACACACGCTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGCTGA
  AACCTTTGAGGAAATGGGAGAAGTTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGTGAC
  CTTCCATGTCATTGCTATCACCTGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAGGAA
  ATCAAGCAGGGTCAGGTAACAGGAATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCATCG
  GCTTCACTGGAGGACTTCTCTTTATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACTCAA
  GGCTTACAATAGAGTGATCTATGTTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAGTCT
  GCACTTACAGAGCCCACCCTTGAAAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTTCTT
  GCACAGAGCCTGAAGACACTGGAGCAGAAATTATTAACGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302385
• ORF Size: 744 bp
• Insert Size: 744
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001302385.1, NP_001289314.1
• RefSeq Size: 4313
• RefSeq ORF: 744
• Locus ID: 71779
• Gene Summary: The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.