Gjb6 (NM_001010937) Mouse Untagged Clone

CAT#: MC226644

Gjb6 (untagged) - Mouse gap junction protein, beta 6 (Gjb6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gjb6
• Synonyms: AA958971; Cx30; D14Bwg0506e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226644 representing NM_001010937
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGGGGACCCTGCACACCGTCATCGGTGGCGTGAACAAGCACTCTACCAGCATAGGGAAGGTGT
  GGATCACGGTCATCTTTATTTTCCGAGTCATGATCCTAGTGGTGGCTGCCCAGGAAGTGTGGGGTGATGA
  GCAGGAGGACTTTGTCTGCAACACTCTGCAGCCAGGGTGCAAGAACGTCTGCTATGACCATTTCTTCCCG
  GTGTCTCACATCCGGCTCTGGGCCCTGCAGCTGATCTTTGTGTCTACCCCAGCCCTGTTGGTGGCCATGC
  ACGTGGCCTACTACAGACATGAAACTGCCCGAAAGTTTATACGTGGGGAGAAGAGAAACGAGTTTAAAGA
  CCTGGAGGACATCAAACGGCAGAAGGTGCGCATTGAGGGCTCCCTGTGGTGGACGTACACCAGCAGCATT
  TTCTTCCGCATCATCTTCGAAGCCGCCTTCATGTATGTGTTCTACTTCCTCTACAATGGGTACCACCTAC
  CCTGGGTACTGAAATGTGGCATTGACCCCTGCCCCAATCTCGTGGACTGCTTCATTTCGAGGCCAACTGA
  GAAAACGGTGTTCACTGTTTTTATGATTTCCGCATCCGTGATTTGCATGCTGCTCAATGTGGCCGAGTTG
  TGTTACCTGCTGCTTAAATTGTGCTTTAGGAGATCCAAAAGAACACAGGCGCAGAGAAACCACCCCAACC
  ATGCCCTGAAAGAGAGCAAGCAGAATGAAATGAATGAGCTGATCTCAGATAGTGGCCAGAATGCAATCAC
  AAGTTTCCCAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010937
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010937.2, NP_001010937.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 786 bp
• Locus ID: 14623
• Cytogenetics: 14 30.1 cM
• Gene Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the shorter transcript. Both variants 1 and 2 encode the same protein.