Sparc (NM_001290817) Mouse Untagged Clone

CAT#: MC226897

Sparc (untagged) - Mouse secreted acidic cysteine rich glycoprotein (Sparc), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sparc
• Synonyms: BM-40; ON
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226897 representing NM_001290817
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGACTGAAGTTG
  CTGAGGAGATAGTGGAGGAGGAAACCGTGGTGGAGGAGACAGGGGTACCTGTGGGTGCCAACCCAGTCCA
  GGTGGAAATGGGAGAATTTGAGGACGGTGCAGAGGAAACGGTCGAGGAGGTGGTGGCTGACAACCCCTGC
  CAGAACCATCATTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGTGCC
  AGGACCCCACCAGCTGCCCTGCTCCCATTGGCGAGTTTGAGAAGGTATGCAGCAATGACAACAAGACCTT
  CGACTCTTCCTGCCACTTCTTTGCCACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTCCAC
  CTGGACTACATCGGACCATGCAAATACATCGCCCCCTGCCTGGATTCCGAGCTGACTGAATTCCCTCTGC
  GCATGCGTGACTGGCTCAAAAATGTCCTGGTCACCTTGTACGAGAGAGATGAGGGCAACAACCTCCTCAC
  TGAGAAGCAGAAGCTGCGTGTGAAGAAGATCCATGAGAATGAGAAGCGCCTGGAGGCTGGAGACCACCCC
  GTGGAGCTGTTGGCCCGAGACTTTGAGAAGAACTACAATATGTACATCTTCCCTGTCCACTGGCAGTTTG
  GCCAGCTGGATCAGCACCCTATTGATGGGTACCTGTCCCACACTGAGCTGGCCCCACTGCGCGCTCCCCT
  CATCCCCATGGAACATTGCACCACACGTTTCTTTGAGACCTGTGACCTAGACAACGACAAGTACATTGCC
  CTGGAGGAATGGGCCGGCTGCTTTGGCATCAAGGAGCAGGACATCAACAAGGATCTGGTGATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290817
• ORF Size: 906 bp
• Insert Size: 906
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001290817.1, NP_001277746.1
• RefSeq Size: 2638
• RefSeq ORF: 906
• Locus ID: 20692
• Gene Summary: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.