Zap70 (NM_001289612) Mouse Untagged Clone

CAT#: MC226959

Zap70 (untagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zap70
• Synonyms: mrtle; mur; Srk; ZAP-70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226959 representing NM_001289612
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGACAAGAAGCTCT
  TCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCTCCGTGCGCCA
  GGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCACAGAGAAG
  GCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTACATCGTGCGGC
  TCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGCCCCTGCACAA
  GTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCAGGTGGCCATG
  GGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTTCTACTGGTCA
  ATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACAGCTATTACAC
  AGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGAAGTTCTCC
  AGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAAGCCCTACA
  AGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTCCGCCGGAGTG
  TCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCCCGACTTCCTG
  ACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCCCCACAGTGTG
  AACAGGTGGCCGAGGCTGCATGTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289612
• ORF Size: 939 bp
• Insert Size: 939
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001289612.1, NP_001276541.1
• RefSeq Size: 1343
• RefSeq ORF: 939
• Locus ID: 22637
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) contains a 5' terminal exon that extends past a splice site that is used in variant 1. This variant differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (TZK, also known as truncated ZAP kinase; PMID 14985102) has a shorter N-terminus compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.