Uba3 (NM_001301859) Mouse Untagged Clone

CAT#: MC227473

Uba3 (untagged) - Mouse ubiquitin-like modifier activating enzyme 3 (Uba3), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba3
• Synonyms: A830034N06Rik; AI256736; AI848246; AW546539; Ube1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227473 representing NM_001301859
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAGAAAATGGCTG
  TTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCG
  GTCTGGACCCTTCACACACCCCGATTTCGAACCAAGCACTGAATCACTCCAGTTCTTGTTAGATACATGT
  AAAGTTCTAGTCATTGGAGCTGGTGGCTTAGGATGTGAGCTTCTGAAAAATCTGGCATTATCTGGTTTTA
  GACAGATTCATGTTATAGACATGGACACTATAGATGTTTCCAATTTAAATAGACAGTTTTTATTTAGGCC
  TAAAGATGTCGGAAGACCCAAGGCTGAAGTTGCTGCAGAATTCCTAAATGACAGAGTTCCTAACTGCAAC
  GTGGTACCACATTTCAACAAGATACAAGATTTTAACGACACTTTCTACCGACAATTTCATATCATTGTAT
  GTGGCCTGGACTCTATCATAGCGAGAAGATGGATCAATGGAATGCTGATATCTCTTCTAAATTATGAAGA
  TGGTGTGTTGGATCCAAGCTCCATTGTACCTTTGATAGATGGGGGGACAGAAGGCTTTAAAGGGAATGCC
  CGAGTGATTTTGCCTGGAATGACCGCTTGTATTGAGTGCACTCTGGAACTTTACCCACCACAGGTCAATT
  TCCCCATGTGTACCATTGCATCTATGCCCAGGCTCCCAGAACACTGTATCGAGTATGTGAGGATGTTGCA
  ATGGCCTAAAGAGCAGCCTTTTGGAGATGGGGTTCCATTAGATGGAGATGACCCTGAACATATTCAGTGG
  ATTTTCCAAAAGTCCATAGAGAGAGCATCACAATATAATATTAGAGGCGTTACCTACAGACTCACTCAAG
  GGGTGGTAAAACGAATCATTCCTGCAGTAGCTTCTACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGA
  GGTTTTCAAGATAGCTACAAGTGCGTACATTCCCCTTAATAACTACCTGGTATTCAATGATGTAGATGGG
  CTGTACACTTACACGTTTGAAGCAGAGAGAAAGGAAAACTGTCCAGCATGTAGCCAACTTCCTCAAAACA
  TTCAGTTTTCCCCATCAGCTAAACTACAGGAGGTCTTAGACTACCTAACCAACAGTGCTTCTCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301859
• ORF Size: 1188 bp
• Insert Size: 1188
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301859.1, NP_001288788.1
• RefSeq Size: 1361
• RefSeq ORF: 1188
• Locus ID: 22200
• Gene Summary: The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (5) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.