Uba3 (NM_001301859) Mouse Untagged Clone

CAT#: MC227473

Uba3 (untagged) - Mouse ubiquitin-like modifier activating enzyme 3 (Uba3), transcript variant 5


  "NM_001301859" in other vectors (1)

Reconstitution Protocol

USD 400.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Uba3
Synonyms A830034N06Rik; AI256736; AI848246; AW546539; Ube1c
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227473 representing NM_001301859
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAGAAAATGGCTG
TTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCG
GTCTGGACCCTTCACACACCCCGATTTCGAACCAAGCACTGAATCACTCCAGTTCTTGTTAGATACATGT
AAAGTTCTAGTCATTGGAGCTGGTGGCTTAGGATGTGAGCTTCTGAAAAATCTGGCATTATCTGGTTTTA
GACAGATTCATGTTATAGACATGGACACTATAGATGTTTCCAATTTAAATAGACAGTTTTTATTTAGGCC
TAAAGATGTCGGAAGACCCAAGGCTGAAGTTGCTGCAGAATTCCTAAATGACAGAGTTCCTAACTGCAAC
GTGGTACCACATTTCAACAAGATACAAGATTTTAACGACACTTTCTACCGACAATTTCATATCATTGTAT
GTGGCCTGGACTCTATCATAGCGAGAAGATGGATCAATGGAATGCTGATATCTCTTCTAAATTATGAAGA
TGGTGTGTTGGATCCAAGCTCCATTGTACCTTTGATAGATGGGGGGACAGAAGGCTTTAAAGGGAATGCC
CGAGTGATTTTGCCTGGAATGACCGCTTGTATTGAGTGCACTCTGGAACTTTACCCACCACAGGTCAATT
TCCCCATGTGTACCATTGCATCTATGCCCAGGCTCCCAGAACACTGTATCGAGTATGTGAGGATGTTGCA
ATGGCCTAAAGAGCAGCCTTTTGGAGATGGGGTTCCATTAGATGGAGATGACCCTGAACATATTCAGTGG
ATTTTCCAAAAGTCCATAGAGAGAGCATCACAATATAATATTAGAGGCGTTACCTACAGACTCACTCAAG
GGGTGGTAAAACGAATCATTCCTGCAGTAGCTTCTACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGA
GGTTTTCAAGATAGCTACAAGTGCGTACATTCCCCTTAATAACTACCTGGTATTCAATGATGTAGATGGG
CTGTACACTTACACGTTTGAAGCAGAGAGAAAGGAAAACTGTCCAGCATGTAGCCAACTTCCTCAAAACA
TTCAGTTTTCCCCATCAGCTAAACTACAGGAGGTCTTAGACTACCTAACCAACAGTGCTTCTCTGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001301859
ORF Size 1188 bp
Insert Size 1188
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
Reference Data
RefSeq NM_001301859.1, NP_001288788.1
RefSeq Size 1361
RefSeq ORF 1188
Locus ID 22200
Gene Summary The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Transcript Variant: This variant (5) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.

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