Glrb (NM_001281969) Mouse Untagged Clone

CAT#: MC227485

Glrb (untagged) - Mouse glycine receptor, beta subunit (Glrb), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glrb
• Synonyms: AI853901; Glyrb; spa; spastic
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227485 representing NM_001281969
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTATAGAGTTAACATTTTCTTGAGACAGAAATGGAATGACCCCAGACTCAAGCTACCTAGTGACT
  TCAGAGGCTCAGATGCACTGACAGTTGACCCCACCATGTATAAGTGCTTGTGGAAACCTGACTTATTCTT
  TGCAAATGAAAAAAGTGCCAATTTTCATGATGTGACCCAAGAAAATATCCTGTTGTTTATCTTTCGGGAT
  GGAGACGTCCTTGTGAGCATGAGGTTGTCTATTACACTTTCATGTCCTCTAGACTTAACTCTGTTTCCCA
  TGGACACACAACGCTGCAAAATGCAACTTGAGAGCTTTGGATATACAACCGATGATTTAAGATTCATCTG
  GCAGTCAGGAGATCCTGTTCAGTTGGAAAAAATTGCTTTACCTCAATTTGATATTAAAAAGGAGGATATC
  GAATATGGCAACTGTACAAAATACTATAAAGGCACTGGTTACTACACTTGTGTGGAGGTCATCTTCACCC
  TGAGGAGACAGGTTGGGTTCTACATGATGGGCGTATATGCACCAACCTTGCTGATTGTGGTTCTCTCCTG
  GCTCTCTTTCTGGATCAACCCTGATGCTAGTGCTGCCAGAGTACCTCTGGGCATCTTCTCCGTGCTCAGT
  TTGGCCTCAGAGTGCACCACCCTCGCAGCCGAGCTTCCTAAAGTGTCTTATGTGAAGGCGCTGGATGTGT
  GGCTCATTGCCTGCCTGCTCTTCGGGTTTGCCTCCCTCGTGGAGTACGCTGTGGTCCAGGTGATGCTGAA
  CAATCCCAAAAGGGTTGAAGCCGAGAAGGCCCGAATAGCTAAGGCTGAGCAAGCAGATGGGAAAGGTGGA
  AACGCAGCTAAGAAGAATACTGTAAACGGCACGGGGACCCCTGTTCATATCAGCACTTTGCAGGTTGGTG
  AGACCAGATGCAAAAAAGTTTGTACTTCCAAGTCTGATTTGAGATCCAATGACTTCAGCATTGTTGGAAG
  CTTACCAAGAGATTTTGAATTATCCAATTATGACTGCTATGGGAAACCCATCGAAGTCAACAATGGACTT
  GGGAAACCACAGGCAAAGAACAAGAAGCCTCCGCCTGCCAAGCCTGTCATCCCAACAGCAGCCAAGCGCA
  TCGACCTTTATGCAAGAGCATTATTTCCTTTCTGCTTCTTGTTCTTCAATGTTATATATTGGTCTATATA
  TTTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281969
• ORF Size: 1197 bp
• Insert Size: 1197
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001281969.1, NP_001268898.1
• RefSeq Size: 2979
• RefSeq ORF: 1197
• Locus ID: 14658
• Gene Summary: This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. This gene is transcribed throughout the central nervous system of neonatal and adult mice. In humans, mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (8) differs in the 5' UTR, uses a downstream start codon and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 7 which is shorter than isoform 1. Variants 7 and 8 encode the same isoform (7).