Cant1 (NM_001025618) Mouse Untagged Clone

CAT#: MC227512

Cant1 (untagged) - Mouse calcium activated nucleotidase 1 (Cant1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cant1
• Synonyms: 5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8; SCAN-1; Shapy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227512 representing NM_001025618
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATCCAGCCCTTTGACCAGCGGGAATGGAATGAGCCTATGCACTCCCTCCGGATCAGTGTAGGGG
  GCCTTCCTGTGCTGGCATCCATGACCAAGGCCACGGACCCTCGCTTCCGCCCCCGCTGGCGGGTGATCCT
  CACGTCCTTTGTGGGTGCTGCCCTCCTCTGGCTGCTGTACTCCCACCATCAGGGCCCAGTGCCAGGCAGG
  CCCCCCACCCACAATGCGCACAATTGGAGGCTCAGCCAGCAGCGCATCTCTCATTACAACGACACCTACC
  CCCTCTCCCCCCCACAGAGGACTCCAGGTGGGATTCGGTACCGAATCGCAGTCATTGCTGACTTGGACAC
  GGGGTCCAGGGCCCAGGAAGAAAACACTTGGTTTAGCTACCTTAAGAAAGGCTACCTGACCCTGTCGGAC
  AGCGGGGACAGGGTCAGCGTGGAGTGGGATAAAGACCACGGGGTCCTAGAGTCCCACCTGGCAGAAAAGG
  GGCGGGGCATGGAGCTCTCAGACCTGATTGTCTTCAATGGGAAACTCTACTCCGTGGATGACCGCACAGG
  GGTCATCTACCAGATCGAAGGCACCAAAGCAGTGCCCTGGGTGATCCTTTCTGATGGCGATGGAACTGTG
  GAGAAGGGTTTCAAAGCTGAATGGCTTGCTGTGAAGGACGAACACCTGTATGTGGGCGGCCTGGGCAAGG
  AGTGGACCACCACGACAGGGGAGGTGATGAACGAGAACCCTGAGTGGGTGAAGGTGGTAGGCCACAGGGG
  CAGTGTGGACCACGAGAACTGGGTGTCCAGCTACAACGCCCTGAGAGCAGCCGCCGGGATCCGACCACCA
  GGCTACCTCATTCACGAGTCCGCCTGCTGGAGCGACACACTGCAGCGCTGGTTCTTCCTGCCTCGTCGGG
  CTAGCCACGAACGCTACAGCGAGAAGGACGATGAACGCAAGGGCAGCAACCTCCTGCTGAGCGCAGCCCA
  GGACTTCAGGGACATCTCTGTGAGGCAAGTGGGGACTCTGATCCCCACCCACGGCTTCTCATCCTTCAAA
  TTCATCCCCAACACGGATGACCAGATCATCGTGGCTCTCAAGTCGGAAGAGGACAATGGCAGAATCGCCA
  CCTATGTCATGGCCTTCACGCTGGACGGCCGCTTCCTGCTTCCAGAGACCAAGATCGGCACTGTGAAGTA
  CGAAGGAATAGAGTTCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025618
• Insert Size: 1212 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025618.2, NP_001020789.1
• RefSeq Size: 2915 bp
• RefSeq ORF: 1212 bp
• Locus ID: 76025
• Cytogenetics: 11 83.15 cM
• Gene Summary: The protein encoded by this gene is a calcium-dependent nucleotidase that preferentially hydrolyzes UDP, GDP, and IDP. The encoded protein has low activity with ADP and ATP and shows no activity with AMP and GMP. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.