Uba3 (NM_001301857) Mouse Untagged Clone

CAT#: MC227721

Uba3 (untagged) - Mouse ubiquitin-like modifier activating enzyme 3 (Uba3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba3
• Synonyms: A830034N06Rik; AI256736; AI848246; AW546539; Ube1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227721 representing NM_001301857
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCC
  TCGAGCGGTCTGGACCCTTCACACACCCCGATTTCGAACCAAGCACTGAATCACTCCAGTTCTTGTTAGA
  TACATGTAAAGTTCTAGTCATTGGAGCTGGTGGCTTAGGATGTGAGCTTCTGAAAAATCTGGCATTATCT
  GGTTTTAGACAGATTCATGTTATAGACATGGACACTATAGATGTTTCCAATTTAAATAGACAGTTTTTAT
  TTAGGCCTAAAGATGTCGGAAGACCCAAGGCTGAAGTTGCTGCAGAATTCCTAAATGACAGAGTTCCTAA
  CTGCAACGTGGTACCACATTTCAACAAGATACAAGATTTTAACGACACTTTCTACCGACAATTTCATATC
  ATTGTATGTGGCCTGGACTCTATCATAGCGAGAAGATGGATCAATGGAATGCTGATATCTCTTCTAAATT
  ATGAAGATGGTGTGTTGGATCCAAGCTCCATTGTACCTTTGATAGATGGGGGGACAGAAGGCTTTAAAGG
  GAATGCCCGAGTGATTTTGCCTGGAATGACCGCTTGTATTGAGTGCACTCTGGAACTTTACCCACCACAG
  GTCAATTTCCCCATGTGTACCATTGCATCTATGCCCAGGCTCCCAGAACACTGTATCGAGTATGTGAGGA
  TGTTGCAATGGCCTAAAGAGCAGCCTTTTGGAGATGGGGTTCCATTAGATGGAGATGACCCTGAACATAT
  TCAGTGGATTTTCCAAAAGTCCATAGAGAGAGCATCACAATATAATATTAGAGGCGTTACCTACAGACTC
  ACTCAAGGGGTGGTAAAACGAATCATTCCTGCAGTAGCTTCTACAAATGCAGTCATTGCAGCTGTGTGTG
  CCACTGAGGTTTTCAAGATAGCTACAAGTGCGTACATTCCCCTTAATAACTACCTGGTATTCAATGATGT
  AGATGGGCTGTACACTTACACGTTTGAAGCAGAGAGAAAGGAAAACTGTCCAGCATGTAGCCAACTTCCT
  CAAAACATTCAGTTTTCCCCATCAGCTAAACTACAGGAGGTCTTAGACTACCTAACCAACAGTGCTTCTC
  TGCAAATGAAGTCTCCGGCTATCACAGCCACATTAGAGGGGAAGAACAGGACACTTTACTTACAGTCAGT
  AACGTCTATTGAAGAACGAACCAGGCCCAATCTTTCCAAAACATTAAAAGAACTGGGACTAGTTGATGGA
  CAAGAACTGGCTGTTGCTGATGTCACTACACCACAGACTGTACTATTCAAACTTCATTTTACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301857
• ORF Size: 1326 bp
• Insert Size: 1326
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301857.1, NP_001288786.1
• RefSeq Size: 2835
• RefSeq ORF: 1326
• Locus ID: 22200
• Gene Summary: The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.