Alpl (NM_001287176) Mouse Untagged Clone

CAT#: MC228162

Alpl (untagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alpl
• Synonyms: Akp-2; Akp2; APTNAP; TNAP; TNSALP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228162 representing NM_001287176
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCACCATTTTTAGTACTGGCCATCGGCACCTGCCTTACCAACTCTTTTGTGCCAGAGAAAGAGA
  GAGACCCCAGTTACTGGCGACAGCAAGCCCAAGAGACCTTGAAAAATGCCCTGAAACTCCAAAAGCTCAA
  CACCAATGTAGCCAAGAATGTCATCATGTTCCTGGGAGATGGTATGGGCGTCTCCACAGTAACCGCTGCC
  CGAATCCTTAAGGGCCAGCTACACCACAACACGGGCGAGGAGACCCGGCTGGAGATGGACAAATTCCCCT
  TTGTGGCCCTCTCCAAGACATATAACACCAACGCTCAGGTCCCTGACAGCGCGGGCACTGCCACTGCCTA
  CTTGTGTGGCGTGAAGGCCAACGAGGGCACAGTGGGAGTGAGCGCAGCCACAGAGCGCACGCGATGCAAC
  ACCACTCAGGGCAATGAGGTCACATCCATCCTGCGCTGGGCCAAGGATGCTGGGAAGTCCGTGGGCATTG
  TGACTACCACTCGGGTGAACCACGCCACACCCAGTGCAGCCTACGCACACTCGGCCGATCGGGACTGGTA
  CTCGGATAACGAGATGCCACCAGAGGCTCTGAGCCAGGGCTGCAAGGACATCGCATATCAGCTAATGCAC
  AATATCAAGGATATCGACGTGATCATGGGTGGCGGCCGGAAATACATGTACCCGAAGAACAGAACTGATG
  TGGAATACGAACTGGATGAGAAGGCCAGGGGTACAAGGCTAGATGGCCTGGATCTCATCAGTATTTGGAA
  GAGCTTTAAACCCAGACACAAGCATTCCCACTATGTCTGGAACCGCACTGAACTGCTGGCCCTTGACCCC
  TCCAGGGTGGACTACCTCTTAGGTCTCTTTGAGCCCGGGGACATGCAGTATGAATTGAATCGGAACAACC
  TGACTGACCCTTCGCTCTCCGAGATGGTGGAGGTGGCCCTCCGGATCCTGACCAAAAACCTCAAAGGCTT
  CTTCTTGCTGGTGGAAGGAGGCAGGATTGACCACGGACATCATGAGGGTAAGGCCAAGCAGGCTCTGCAT
  GAAGCAGTGGAGATGGACCAGGCCATTGGCAAGGCAGGCGCCATGACATCCCAGAAAGACACCTTGACTG
  TGGTTACTGCTGATCATTCCCACGTTTTCACATTCGGTGGATACACCCCCCGGGGCAACTCCATCTTTGG
  TCTGGCTCCCATGGTGAGCGACACGGACAAGAAGCCCTTCACGGCCATCCTATATGGTAACGGGCCTGGC
  TACAAGGTGGTGGACGGTGAACGGGAAAATGTCTCCATGGTAGATTACGCTCACAACAACTACCAGGCCC
  AGTCCGCTGTTCCCCTGCGCCATGAGACCCACGGTGGAGAAGACGTGGCGGTCTTTGCCAAGGGCCCGAT
  GGCACACCTGCTTCACGGCGTCCATGAGCAGAACTACATTCCCCATGTGATGGCGTATGCCTCCTGCATT
  GGGGCCAACCTTGACCACTGTGCCTGGGCCGGCTCTGGGAGCGCACCCTCCCCAGGGGCCCTGCTGCTTC
  CACTGGCTGTGCTCTCCCTACGCACCCTGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287176
• ORF Size: 1575 bp
• Insert Size: 1575
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001287176.1, NP_001274105.1
• RefSeq Size: 2672
• RefSeq ORF: 1575
• Locus ID: 11647
• Gene Summary: This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]