Sphk2 (NM_203280) Mouse Untagged Clone

CAT#: MC228480

Sphk2 (untagged) - Mouse sphingosine kinase 2 (Sphk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sphk2
• Synonyms: C76851
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228480 representing NM_203280
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACC
  CGGCCAACGGCCCACGGTTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGACTACGCCCAAA
  GCCAGAAGCCCGGCCCCGAGATGGTCTAGTCTCTCTGGATGAGGTCTCGGGCTGTGGCACCCTGCAGAGC
  CGTAGCCCCGAGGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGTCGAGGGGGCCGGC
  GCAGAGCTACGCGGACCTTCCGGGCGGATGGGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCG
  CTGGGCCACTGCCCTCACGTGTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAA
  TTGCTGCCCCGGAAGCCCAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGC
  GCTGTATGGACCACGTGGTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACG
  ACAGAACCATGCCCGTGAGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCT
  GGAGACGGGCTGCTTTACGAGGTGCTGAATGGGCTCCTTGATCGGCCAGACTGGGAGGATGCCGTGCGGA
  TGCCCATTGGTGTCCTCCCCTGTGGATCGGGCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTT
  TGAGCAGGTTGTCGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCT
  CTGGACCTGCTCTCTGTGACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGAT
  TCTTGTCAGATGTGGACATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCACACTGGGTGC
  AGTGCTAGGCCTGGCCTCGTTGCATACCTACCGTGGACGCCTCTCCTACCTCCCCGCTACCACAGAACCA
  GCCTTGCCCATCCCAGGCCACAGTCTGCCTCGAGCCAAGTCAGAACTAGTCTTGGCTCCAGCCCCAGCCC
  CCGCCGCCACCCACTCGCCTCTACATCGATCTGTGTCTGACCTGCCCCTGCCCCTTCCCCAGCCTGCCTT
  GGTCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGA
  GACTGGGGAGGAGCTGGGGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCA
  AAACAGCTCAGCTTTCACCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGTTCCTGCCTCC
  CACCCACAGTGCCCCAGAAGCCTCTACCTGGGGCCCAGTGGACCACCTCCTCCCTCCCCTGGGCTCTCCA
  CTGCCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGGCATCTTGCCGAGCCACCTCT
  GCGCAGACCTGATGGCAGCCCCACATGCACGCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAG
  CGGCATCTCACGGGCTGCACTTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGC
  TGCCCCCATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCA
  CTGTAGATGGGGAGTTAGTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCGCCACGCTGCT
  CACTGGGCCTGCAGGTCAAAAGCCACAAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_203280
• ORF Size: 1854 bp
• Insert Size: 1854
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_203280.3, NP_975009.1
• RefSeq Size: 3552
• RefSeq ORF: 1854
• Locus ID: 56632
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.