Zap70 (NM_001289766) Mouse Untagged Clone

CAT#: MC228561

Zap70 (untagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zap70
• Synonyms: mrtle; mur; Srk; ZAP-70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228561 representing NM_001289766
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAATCTGTCTTGGAAAACCAGGAAGGAGCCTGTTACCCTCTGTGGTGTGCTTACTACAGGGTCCAG
  CGATGCCCGATCCCGCGGCGCACCTGCCATTCTTCTATGGCAGCATCTCGCGGGCTGAGGCCGAGGAGCA
  CCTGAAGCTGGCAGGCATGGCCGACGGGCTGTTCCTCCTGCGCCAGTGTTTGCGCTCCCTGGGCGGCTAC
  GTGCTGTCGTTGGTGCACGACGTGCGCTTCCACCATTTCCCCATCGAGCGCCAACTCAACGGCACGTACG
  CCATCGCGGGCGGGAAGGCGCACTGCGGCCCGGCCGAGCTCTGCCAGTTCTACTCTCAGGACCCCGACGG
  GCTGCCCTGCAACCTGCGTAAGCCGTGTAACCGGCCGCCCGGACTGGAGCCACAGCCCGGGGTCTTCGAC
  TGCCTGCGTGATGCTATGGTGCGCGACTACGTGCGCCAGACCTGGAAGCTGGAGGGCGATGCACTAGAGC
  AGGCCATCATCAGCCAGGCCCCACAGGTGGAGAAGCTCATTGCTACCACAGCCCACGAGCGAATGCCCTG
  GTATCACAGCAGCCTGACTCGTGAGGAGGCCGAGCGCAAACTCTATTCCGGCCAGCAGACCGACGGCAAG
  TTCCTGCTGAGGCCCCGGAAGGAGCAGGGCACATATGCACTGTCCCTGGTCTATGGGAAAACTGTATACC
  ACTATCTCATCAGCCAGGACAAGGCTGGCAAGTACTGCATCCCCGAAGGCACCAAGTTTGACACGCTCTG
  GCAGCTGGTGGAGTACCTGAAGCTGAAGGCGGATGGACTAATTTACCGCTTGAAGGAGGTCTGTCCCAAC
  AGCAGCGCCAGCGCGGCAGTTGCTGCACCCACCCTCCCAGCCCACCCATCCACATTCACTCAGCCTCAGA
  GACGAGTGGACACCCTGAACTCGGACGGATACACCCCTGAACCAGCACGCTTAGCGTCATCGACAGACAA
  GCCCCGGCCGATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGAC
  AAGAAGCTCTTCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCT
  CCGTGCGCCAGGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGG
  CACAGAGAAGGCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTAC
  ATCGTGCGGCTCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGC
  CCCTGCACAAGTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCA
  GGTGGCCATGGGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTT
  CTACTGGTCAATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACA
  GCTATTACACAGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCG
  GAAGTTCTCCAGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAG
  AAGCCCTACAAGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTC
  CGCCGGAGTGTCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCC
  CGACTTCCTGACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCC
  CCACAGTGTGAACAGGTGGCCGAGGCTGCATGTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289766
• ORF Size: 1929 bp
• Insert Size: 1929
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001289766.1, NP_001276695.1
• RefSeq Size: 2255
• RefSeq ORF: 1929
• Locus ID: 22637
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (4) encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.