Atg7 (NM_001253718) Mouse Untagged Clone

CAT#: MC228712

Atg7 (untagged) - Mouse autophagy related 7 (Atg7), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg7
• Synonyms: 1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228712 representing NM_001253718
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACCCTGGACTGGCCAAGTTGCAGTTCGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGC
  ACGAACTGACCCAGAAGAAGTTGAACGAGTACCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTA
  CTACAATGGTGACTCTGCTGGTCTGCCCACCCGCTTGACGTTGGAGTTCAGTGCTTTTGACATGAGTGCC
  TCCACGCCTGCCCACTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGA
  CAGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCAAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGC
  TCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCTCCTGACCTTCGCGGACCTAAAGAAGTACCACTTC
  TACTACTGGTTTTGCTGCCCCGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGAGCT
  TGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTATGATGATCTGTGTCGAGCCGA
  AGGCGTCACGGCCCTGCCCTACTTCTTATTCAAGTACGATGACGACACTGTTCTGGTCTCCTTGCTCAAA
  CACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAGATAACAGTTGGTGTGTACGATCCCTGTAACCTAG
  CCCAGTACCCTGGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCA
  GTCCGTTGAAGTCCTCTGCTTTCGGGACCGCACCATGCAGGGAGCTAGAGACGTGACACATAGCATCATC
  TTTGAAGTGAAACTTCCAGAAATGGCATTTAGCCCAGATTGTCCTAAAGCTGTTGGCTGGGAGAAGAACC
  AGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCAGTGGATGTATGGACCCCAAAAGGCTGGCTGAGTC
  ATCTGTGGATCTGAATCTCAAGCTGATGTGCTGGCGATTGGTCCCCACCTTGGACTTGGACAAGGTCGTG
  TCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGGACACTGATGGGCTGGG
  GCGTCAGACATGTCACCTTTGTGGATAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTA
  TGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTACAGAAAATA
  TTTCCCGGAGTGAATGCCAGAGGGTTCAACATGAGCATCCCCATGCCAGGACACCCTGTGAACTTCTCTG
  ACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGCAGCTCATTGATAACCATGATGTCAT
  CTTCCTGCTAATGGACACCAGGGAGAGCCGGTGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTG
  GTCATCAACGCTGCCTTGGGGTTTGATACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGC
  AGGGAGCCGGAGACCTCTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAA
  CATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCAACCAGAGAC
  CGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCAGGCCTGGCCGTGATTGCAGGTGCCCTGGCTGTGG
  AGCTGATGGTCTCTGTCCTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCAT
  GAATGAGCCTCCCACCTCGCTGGGACTTGTGCCTCACCAGATCCGGGGTTTTCTGTCACGGTTCGATAAT
  GTTCTTCCTGTCAGCCTGGCATTTGATAAATGTACAGCCTGTTCACCCAAAGTTCTTGATCAGTACGAGC
  GAGAAGGATTCACCTTCCTAGCGAAGGTTTTTAACTCCTCACATTCCTTCTTAGAAGACTTGACCGGTCT
  TACCCTGCTCCATCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACTGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253718
• ORF Size: 2097 bp
• Insert Size: 2097
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001253718.1, NP_001240647.1
• RefSeq Size: 3895
• RefSeq ORF: 2097
• Locus ID: 74244
• Gene Summary: This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, resulting in initiation of translation from a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.