Hgf (NM_001289461) Mouse Untagged Clone

CAT#: MC228773

Hgf (untagged) - Mouse hepatocyte growth factor (Hgf), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hgf
• Synonyms: C230052L06Rik; HGF/SF; NK1; NK2; SF; SF/HGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228773 representing NM_001289461
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTCCTGCACCTCCTCCTGCTTC
  ATGTCGCCATCCCCTATGCAGAAGGACAGAAGAAAAGAAGAAATACACTTCATGAATTTAAAAAGTCAGC
  AAAAACTACTCTTACCAAGGAAGACCCATTACTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG
  TGTGCCAACAGGTGTATCAGGAACAGGGGCTTTACGTTCACTTGCAAGGCCTTCGTTTTTGATAAGTCAA
  GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT
  TGACCTCTATGAAAACAAAGACTATATTAGAAACTGCATCATTGGTAAAGGAGGCAGCTATAAAGGGACG
  GTATCCATCACTAAGAGTGGCATCAAATGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTATC
  GCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGGACCCTGGTGTTTCAC
  AAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTTGAATGCATGACCTGC
  AATGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACTTGTCAGCGCTGGGACCAGC
  AGACACCACACCGGCACAAGTTCTTGCCAGAAAGATATCCCGACAAGGGCTTTGATGATAATTATTGCCG
  CAATCCTGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCTTGGGAGTATTGTGCA
  ATTAAAACGTGCGCTCACAGTGCTGTGAATGAGACTGATGTCCCTATGGAAACAACTGAATGCATTCAAG
  GCCAAGGAGAAGGTTACAGGGGAACCAGCAATACCATTTGGAATGGAATTCCCTGTCAGCGTTGGGATTC
  GCAGTACCCTCACAAGCATGATATCACTCCCGAGAACTTCAAATGCAAGGACCTTAGAGAAAATTATTGC
  CGCAATCCAGATGGGGCTGAATCACCATGGTGTTTTACCACTGACCCAAACATCCGAGTTGGCTACTGCT
  CTCAAATTCCCAAGTGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGGGAAAAATTACATGGG
  CAACTTATCCAAAACAAGGTCTGGACTTACATGTTCCATGTGGGACAAGAATATGGAGGATTTACACCGT
  CATATCTTCTGGGAGCCAGATGCTAGCAAATTGAATAAGAATTACTGCCGGAATCCTGATGATGATGCCC
  ATGGACCTTGGTGCTACACGGGGAATCCTCTTATTCCTTGGGATTATTGCCCTATTTCCCGTTGTGAAGG
  AGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAAACAAAACAACTGCGG
  GTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAATACAGAAATAAACATA
  TCTGTGGAGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGACAATGTTTTCCAGCCAGAAACAA
  AGACTTGAAAGACTATGAAGCTTGGCTTGGCATCCACGATGTTCATGAGAGAGGCGAGGAGAAGCGCAAG
  CAGATCTTAAACATTTCCCAGCTGGTCTATGGTCCTGAAGGCTCAGACTTGGTTTTACTGAAGCTTGCTC
  GACCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGTTGTACAATCCCTGAAAA
  GACCACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCGGATGGTTTATTACGAGTAGCT
  CATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGACTTTGAATGAGTCTG
  AGTTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCATGTGAGGGAGATTATGGTGGCCCACTCATTTG
  TGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGTGCCATCCCAAATCGT
  CCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTTTGACATACAAGTTGT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289461
• ORF Size: 2172 bp
• Insert Size: 2172
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001289461.1, NP_001276390.1
• RefSeq Size: 2645
• RefSeq ORF: 2172
• Locus ID: 15234
• Gene Summary: This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.