Plk4 (NM_173169) Mouse Untagged Clone

CAT#: MC229135

Plk4 (untagged) - Mouse polo-like kinase 4 (Plk4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plk4
• Synonyms: 1700028H20; AI385771; Sak; Stk18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229135 representing NM_173169
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTGCATCGGGGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTCGGTAAAGGATCATTTG
  CTGGTGTCTACAGAGCTGAGTCCATACACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC
  CATGTACAAAGCTGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAGTTGAAACACCCC
  TCTGTCTTGGAGCTCTATAATTACTTTGAAGATAACAATTATGTCTACCTGGTATTGGAAATGTGCCACA
  ATGGAGAAATGAACAGATATCTGAAGAACAGAATGAAGCCTTTCTCAGAAAGGGAAGCTAGGCACTTCAT
  GCACCAGATTATCACAGGAATGTTATATCTTCATTCTCATGGCATATTGCACCGGGACCTCACACTCTCT
  AACATCTTACTTACGCGGAATATGAACATAAAAATTGCTGACTTTGGACTAGCAACGCAGTTGAATATGC
  CACATGAAAAGCACTATACACTCTGTGGGACTCCTAATTATATTTCACCAGAAATTGCAACTCGAAGTGC
  ACATGGACTTGAATCTGATATTTGGTCATTGGGCTGTATGTTTTATACGTTACTTATTGGAAGACCACCT
  TTTGACACTGACACAGTCAAGAACACATTGAACAAAGTAGTCCTGGCAGATTATGAAATGCCAGCCTTTT
  TGTCACGAGAGGCCCAGGACCTTATCCACCAGTTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCTGTC
  TTCTGTGTTGGACCATCCTTTCATGTCACGAAATCCTTCACCAAAGAGTAAAGACGTAGGGACTGTAGAG
  GACTCAATGGATAGTGGGCATGCTACACTTTCCACAACAATTACAGCCTCTTCTGGTACCAGTTTGAGTG
  GCAGCCTACTTGACAGAAGACTTTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAAAAA
  TAAAAATTCAAGTGACTTTTCTTCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAACAA
  GAAGCTAATAGTAGGGGACGGGGGAGAGTGATTGAAGATGCAGAAGAGAGGCCGCATTCTCGATACCTGC
  GCAGAGCTCATTCCTCTGATAGAGCCAGCCCCTCTAATCAGTCTCGAGCAAAAACATACTCAGTAGAACG
  TTGTCACTCAGTAGAAATGCTTTCAAAGCCTAGAAGATCACTGGATGAAAATCAACACAGTTCCAATCAT
  CATTGTCTAGGAAAAACTCCTTTTCCATTTGCAGACCAGACACCTCAGATGGAAATGGTACAGCAGTGGT
  TTGGGAATCTGCAAATGAATGCTCATTTAGGAGAAACTAATGAGCACCACACCGTTAGCCCAAACAGAGA
  TTTCCAGGACTATCCAGATTTGCAGGACACGTTACGAAACGCTTGGACTGACACGAGAGCCAGCAAGAAT
  GCTGATACTTCTGCCAATGTTCATGCTGTAAAGCAGCTGAGTGCCATGAAATACATGAGTGCACATCACC
  ATAAGCCTGAGGTCATGCCACAGGAGCCGGGCCTACATCCTCATTCTGAACAAAGCAAGAATAGAAGTAT
  GGAGTCGACACTGGGTTACCAGAAACCTACCTTAAGAAGTATTACATCTCCTCTGATTGCTCACAGATTA
  AAGCCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATCCTTGATTCAGAGGAGATCACTGTTTATT
  ACCCGAACGATGGAAGAGGCTTTCCTCTTGCTGACAGACCTCCCTTGCCTACTGACAACATCAGTAGGTA
  CAGCTTTGACAATCTACCAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGATTCATTCAGCTAGTA
  AGATCTAAAACTCCCAAAATCACTTATTTTACAAGATATGCTAAATGTATTTTGATGGAAAATTCTCCTG
  GTGCTGATTTCGAAGTTTGGTTTTATGATGGAGCCAAAATACATAAAACTGAAAATTTAATTCACATAAT
  TGAGAAAACAGGGATATCTTATAATTTAAAAAATGAAAATGAAGTTACCAGCCTGAAAGAGGAAGTAAAA
  GTATATATGGACCATGCTAATGAGGGTCACCGTATTTGCTTGTCACTGGAATCTGTAATCTCTGAGGAGG
  AAAAGAGAAGCAGGGGTTCTTCATTCTTCCCTATAATCGTAGGAAGAAAACCTGGTAATACTAGTTCACC
  TAAAGCCTTATCAGCTCCTCCTGTGGACCCAAGCTGCTGTAAGGGAGAGCAGGCGTCAGCAAGCAGACTG
  AGCGTGAATAGTGCCGCTTTCCCCACACAGTCCCCAGGACTCAGTCCTTCCACTGTGACAGTTGAAGGAC
  TTGGCCACACAGCGACTGCCACAGGAACAGGCGTCTCTTCAAGTCTTCCTAAATCTGCACAGCTTTTGAA
  ATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGCTAACTAGCGGAGCTGTGTGGGTTCAGTTTAAT
  GATGGGTCACAGTTGGTTGTCCAGGCAGGAGTATCTTCCATCAGTTACACATCACCAGATGGTCAGACAA
  CTAGGTATGGAGAAAATGAAAAATTACCTGAATACATCAAACAGAAATTACAGTGTCTTTCTTCCATCCT
  TCTGATGTTTTCTAATCCAACTCCTAATTTTCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173169
• ORF Size: 2697 bp
• Insert Size: 2697
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_173169.2, NP_775261.2
• RefSeq Size: 3486
• RefSeq ORF: 2697
• Locus ID: 20873
• Gene Summary: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2, to centrosomes during G2/M, and to the cleavage furrow during cytokinesis. It is required for progression through mitosis, cell survival, and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The transcription start site was identified in PMID:10607900.