Dnmt3a (NM_001271753) Mouse Untagged Clone

CAT#: MC229155

Dnmt3a (untagged) - Mouse DNA methyltransferase 3A (Dnmt3a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnmt3a
• Synonyms: MmuIIIA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229155 representing NM_001271753
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG
  AGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCCAGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAG
  GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCAGTGACCACC
  AAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA
  GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG
  AACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGAGAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGA
  GCCTCTGCAGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG
  GCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCCTCCGTCAGCGACCCATGCCAAGACTCACCTTCCA
  GGCAGGGGACCCCTACTACATCAGCAAACGGAAACGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT
  GAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCTGTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGA
  AGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACGGACCCTGCTTCTCCGACTGTGGCCACCAC
  CCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAATGCTACCAAAGCAGCCGACGATGAGCCTGAGTAT
  GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAG
  GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTT
  CGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAAGCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTC
  CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA
  GCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACAGTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGT
  GCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGGCTTCCAGCCCTCGGGTCCTAAGGGCCTGGAGCCA
  CCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTT
  ACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCTAAGGTCAAGGAGATCAT
  TGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGTGCGCCAGAAGTGCAGAAACATCGAGGACATTTGT
  ATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCACCCACTCTTCATTGGAGGCATGTGCCAGAACTGTA
  AGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACGACGATGGGTACCAGTCCTATTGCACCATCTGCTG
  TGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGAT
  CTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGC
  ATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAGACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAA
  TAACCATGACCAGGAATTTGACCCCCCAAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC
  CGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG
  ACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGAAAGAT
  CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGACCTGGTG
  ATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTCAACCCTGCCCGCAAGGGACTTTATGAGGGTACTG
  GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT
  CTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTTGAG
  TCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTACTTCTGGGGTAACC
  TTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGAATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCA
  CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCTATAAAGCAGGGCAAA
  GACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAGGACATCCTGTGGTGCACTGAAATGGAAAGGGTGT
  TTGGCTTCCCCGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG
  ATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271753
• ORF Size: 2727 bp
• Insert Size: 2727
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001271753.1, NP_001258682.1
• RefSeq Size: 9689
• RefSeq ORF: 2727
• Locus ID: 13435
• Gene Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same isoform (1, also known as Dnmt3a1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.