Usp15 (NM_001301628) Mouse Untagged Clone

CAT#: MC229208

Usp15 (untagged) - Mouse ubiquitin specific peptidase 15 (Usp15), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp15
• Synonyms: 4921514G19Rik; AI327321; E430033I05Rik; Gcap18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229208 representing NM_001301628
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC
  TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
  TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT
  CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA
  CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT
  GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG
  AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG
  AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATATGAGCAACAC
  ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG
  ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAATGTGAAAAACTCAAATT
  ATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGAAGAAACAATGAACAGCC
  AGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAAC
  ACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAG
  GAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTA
  CGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCTCTGGATATCAACAGCAG
  GACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAATAGAATTAGGAAAAAAC
  CCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCA
  TTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCT
  GAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAG
  AACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATGCAATACAAAGTTATTGT
  GCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAG
  ATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTA
  TTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAAGACACAGAGCATGTAGT
  TATCCCTGTCTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACTCACCACACTGGCTCTTCACTGTTC
  GGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTATAATCTTCTACTCTTGA
  GAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGA
  CCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGAC
  GAGCCAGATGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATT
  CAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGG
  ACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACATCAACTACATCAAAGAC
  GACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACT
  GGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGT
  GGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAG
  AAGCTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAG
  ATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGA
  CAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAATTCCTAATTAACCCAAAT
  GCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACATTATA
  CTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGTAGCGTCTCCACTGCATC
  TGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACT
  GGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACG
  AAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACTAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301628
• ORF Size: 2859 bp
• Insert Size: 2859
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301628.1, NP_001288557.1
• RefSeq Size: 11477
• RefSeq ORF: 2859
• Locus ID: 14479
• Gene Summary: The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.