Slit2 (NM_001291227) Mouse Untagged Clone

CAT#: MC229625

Slit2 (untagged) - Mouse slit homolog 2 (Drosophila) (Slit2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slit2
• Synonyms: b2b1200.1Clo; Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; Slil3; slit-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229625 representing NM_001291227
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCGCTGGGGTTAGTGTTGTCGATCTTGAACAAGGTGG
  CGCCGCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACGGTGGACTGTCATGGGCTGGCACTGCG
  CAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTGAATGGAAATAACATCACGAGGATC
  ACGAAGATAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCAGCTCATGGAGAACAGAATCAGCACCA
  TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT
  GTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAAATTCAA
  GCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAACCTGCAACTGGATTACAACCAGATCA
  GCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT
  TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCGAACAAC
  TTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA
  CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG
  CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCCGCTGCT
  TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG
  AGACCATCACAGAAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGAGCCTTCTCACCATA
  CAAAAAGCTTAGACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAGATGCCTTCCAAGGA
  CTGCGCTCTCTGAATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTCGAAG
  GACTATTTTCCTTGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGACCTGCACAACTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTGCCAAGGGCACCTTC
  TCAGCCCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT
  GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGCCCCCGCCGCCT
  GGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGCTAAAGAACAGTATTTCATT
  CCAGGTACAGAAGATTATCGATCAAAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAGAAGT
  GTCGCTGTGAAGGGACCACAGTAGACTGCTCCAATCAAAGACTCAACAAAATCCCTGACCATATTCCCCA
  GTACACAGCAGAGCTGCGTCTCAATAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAA
  CTTCCTCAGTTACGTAAAATCAACTTTAGCAACAATAAGATCACGGATATCGAGGAGGGTGCATTTGAAG
  GCGCGTCTGGTGTGAATGAAATTCTTCTCACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAA
  AGGACTGGAGAGCCTCAAAACATTGATGCTGAGAAGTAATCGAATAAGCTGTGTTGGGAACGACAGTTTC
  ATAGGACTCGGCTCTGTGCGTCTGCTCTCTTTATATGACAATCAAATTACCACAGTGGCACCAGGAGCAT
  TTGATTCTCTCCATTCATTATCCACTCTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGC
  ATGGCTGGGAGAATGGCTCAGAAGGAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTC
  CTGAAGGAAATCCCAATCCAGGATGTAGCCATTCAGGACTTCACCTGTGATGATGGAAATGATGACAATA
  GTTGCTCTCCACTCTCCCGTTGTCCTTCTGAATGTACCTGCTTGGATACAGTGGTACGATGTAGCAACAA
  GGGCTTGAAGGTTTTGCCTAAAGGTATTCCAAAAGATGTCACAGAGCTGTATCTGGATGGGAACCAGTTT
  ACGCTGGTCCCGAAGGAACTCTCTAACTACAAACATTTAACACTTATAGACTTAAGTAACAACCGAATAA
  GCACCCTTTCCAATCAAAGCTTCAGCAACATGACCCAGCTTCTCACCTTAATCCTCAGTTACAACCGTCT
  GAGATGTATCCCTCCACGAACCTTTGATGGATTGAAGTCTCTTCGGTTACTGTCTTTACATGGAAATGAC
  ATTTCTGTTGTGCCTGAAGGTGCCTTCAATGACTTGTCAGCCTTGTCACACTTAGCGATTGGAGCCAACC
  CTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAAT
  TGCACGCTGTGCCGGCCCTGGAGAAATGGCAGATAAATTATTACTCACTACTCCCTCCAAAAAATTTACA
  TGTCAAGGTCCCGTGGATATCACTATTCAAGCCAAGTGTAATCCCTGCTTATCAAATCCATGTAAAAATG
  ATGGCACCTGTAACAATGACCCCGTTGATTTTTATCGATGTACCTGCCCATATGGATTCAAGGGTCAGGA
  CTGTGATGTCCCCATTCATGCTTGTATCAGTAATCCATGTAAACATGGAGGAACTTGTCACTTAAAGGAA
  GGAGAGAATGCTGGATTCTGGTGCACTTGTGCTGATGGGTTTGAAGGAGAAAACTGTGAAGTCAATATTG
  ATGATTGTGAAGATAATGATTGTGAAAATAATTCTACATGCGTTGATGGAATTAACAACTACACATGTCT
  TTGCCCACCGGAATACACAGCTGCTAATCTGAATGAGGTGGAAAAAGGTGAACTGTGTGAGGAAAAGCTG
  GACTTCTGTGCACAAGACTTGAATCCCTGCCAGCATGACTCCAAGTGCATCCTGACTCCAAAGGGATTCA
  AGTGTGACTGCACTCCAGGATACATTGGTGAGCACTGTGACATTGACTTTGATGACTGCCAAGATAACAA
  GTGTAAAAACGGTGCTCACTGCACAGATGCCGTGAACGGATACACGTGCGTCTGTCCTGAAGGCTACAGT
  GGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCCTGTGATAATTTTGATTGCC
  AGAATGGAGCCCAGTGTATCATCAGGATAAATGAACCAATATGCCAGTGTTTGCCTGGCTACCTGGGAGA
  GAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCTTCAGATTCCTTCAGCCAAG
  GTTCGGCCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGCGGCATCCTCTTGTATAAAG
  GTGACAAAGACCACATTGCCGTGGAACTCTATAGAGGGCGAGTTCGAGCCAGCTATGACACCGGCTCTCA
  TCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAGCTACTGACC
  CTGGATTCCAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAGTCATCACCAATTTGTCAAAACAAT
  CTACTCTGAATTTCGACTCTCCACTCTATGTAGGAGGCATGCCTGGGAAAAATAACGTGGCATCCCTGCG
  CCAGGCCCCTGGGCAAAATGGCACCAGCTTCCATGGCTGTATCCGGAACCTTTACATTAACAGTGAGCTG
  CAGGACTTCCGGAAAATGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAACCATGCCACAAGAAAGTAT
  GTGCCCATGGCATGTGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAATGTGAGGAAGGGTGGATGGG
  GCCCCTCTGTGACCAGAGAACCAATGATCCCTGCCTCGGAAACAAATGTGTGCATGGGACCTGCCTGCCC
  ATCAATGCCTTCTCCTATAGTTGCAAGTGCCTGGAGGGCCATGGCGGTGTCCTCTGTGATGAAGAAGAAG
  ATCTCTTTAACCCCTGCCAGATGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCTGGAGTGGGCCAGCC
  CTATTGTGAATGCAACAGTGGATTCACCGGGGACAGCTGTGATAGAGAAATTTCTTGTCGAGGGGAACGG
  ATAAGGGACTATTACCAGAAGCAGCAGGGTTACGCTGCCTGTCAAACAACTAAGAAAGTATCTCGCTTGG
  AATGCAGAGGCGGGTGCGCTGGAGGCCAGTGCTGTGGACCTCTGAGAAGCAAGAGGCGGAAATACTCTTT
  CGAATGCACAGATGGCTCCTCATTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGCGCGAGATGT
  GCCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291227
• ORF Size: 4629 bp
• Insert Size: 4629
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001291227.2, NP_001278156.1
• RefSeq Size: 10021
• RefSeq ORF: 4629
• Locus ID: 20563
• Gene Summary: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.