Dnmt1 (NM_001199431) Mouse Untagged Clone

CAT#: MC229641

Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnmt1
• Synonyms: Cxxc9; Dnmt; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD2; MTase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229641 representing NM_001199431
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG
  TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA
  CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA
  GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA
  ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC
  AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG
  GACAGTGACACCCTTTCAGTTGAAACTTCACCTAGTTCCGTGGCTACGAGGAGAACCACCAGGCAGACCA
  CCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGAAGAGTCGGAAGAGGGGAACTC
  GGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTTGTAGACACAGAGAGTGGTGCT
  GCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGGGTCCGGAAGAGCCATGTGAAC
  AGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATTGAGGCGGAAATCAAAGGAGGA
  TCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGACGGAAAAAAGGATAAAAGAAGT
  TCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCAAGGAAGCAGAGCCAGAGCAGG
  TAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAAGAGACGAAAAACGACACGTAA
  AAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAAGCCGCTCAAAGCAAAAGTGTG
  ATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAGACGACCCTAACCTGAAGTACC
  AGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGAGAAACTGTCCATCTACGACTC
  CACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTCACTTCCTTCAGTGTGTACTGC
  AGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATGTAGAGCTCTACTTTTCTGGGT
  GTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAATGGCAAAAACCTCGGGCCAAT
  CAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATTGGCTTCTCCACTGCATTTGCT
  GAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGCTGATGCAGGAGAAAATTTACA
  TCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATATGAAGACCTGATCAATAAGAT
  TGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAGGACTCCCTCTTACGCCACGCC
  CAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATGATGAGACCCCCATCTTCTTGT
  CTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACAGAGGCGAGCAACAAGGCGCGT
  CATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACCACCAAGCTGGTCTATCAGATC
  TTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACAAGGAGAATGCCATGAAGCGCC
  GCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGATATGGT
  GAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCGGTGAAG
  GAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCATCACCCAAAAAGCTGCATCAGG
  GGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCCTATGAAGATTGAAGAGAATAG
  AACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGCGACTGCGTCTCGGTCATTCCA
  GATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGGAAGACAAAAATGGTCAGATGA
  TGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGCCACCTCCGACCCCCTGGAACT
  GTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAAA
  GCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCACACTGCCTGGGGCTGAGGATG
  GCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTTTGAATCCCCACCCAAGACCCA
  GCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAA
  ATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACTGCAGTTCCATCACCAAGAATG
  GTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTTTACTTTCAACATCAAAGTGGC
  TAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTACCCTGAGCACTACCGCAAGTAT
  TCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCATCGGTCGGATAAAAGAGATCC
  ACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAGGCTCTACAAGTTCTACAGGCC
  TGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAACATGCTTTACTGGAGCGACGAG
  GAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGTACGGGGAAGACCTACTTGAGA
  GCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGAGGCCTACAATTCAAAGACCAA
  GAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGGAAAGGGAAGGGGAAAGGGAAG
  GGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCATCAAACTGCCCAAGCTCCGGA
  CCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCAAGCAGGCATCTCGGAAACGCT
  GTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAACAACCCCGGCACCACAGTGTTC
  ACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGACCAACTCTCTGGGCCAAAGGC
  TGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCAGGGCTTCAGTGGCATGAACCG
  CTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCCTTCCTCAGCTACTGTGACTAC
  TACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCTACAGACGCTCCATGGTGCTGA
  AGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGGTGTGCTCCAGGCTGGACAGTA
  TGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCAGGAGAAAAGCTGCCTCTGTTC
  CCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTGTGGTGGATGACAAGAAGTTTG
  TTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGTGCGAGACACCATGTCTGACCT
  CCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGAGAGCCACTGTCCTGGTTCCAG
  AGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCCCACTGG
  TGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCGTGACCTGCCCAACATACAGGT
  GCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCATGATGTGAAAAATGGCTACAGC
  AGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCTGCGACCCTGAGTCCAGGCAAT
  TCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTACGG
  GCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAGCCCATGGGCAAGCAGGGTCGG
  GTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCCGCTCCCAGGGCTTTCCAGATA
  GCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAATGCTGTGCCACCACCCCTGGC
  CAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGGGAGAGCGCATCAGCTGCAGTT
  AAAGCAAAAGAGGAGGCTGCTACCAAGGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199431
• ORF Size: 4863 bp
• Insert Size: 4863
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001199431.1, NP_001186360.2
• RefSeq Size: 5370
• RefSeq ORF: 4863
• Locus ID: 13433
• Gene Summary: This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1). This variant has two upstream in-frame AUGs but N-terminal sequencing and western analysis from PMID: 9830015 (Table I and Figure 2) support the use of the third in-frame AUG.