(BC025114) Mouse Tagged ORF Clone

CAT#: MG200384

(GFP-tagged) - Mouse cDNA clone MGC:35756 IMAGE:4925089

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Synonyms: 1810008A14Rik|PIG-Y|Pigy
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200384 representing BC025114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGTCGGGACAAGGGGAAGATAAGACAAGAGCGCACCCGCATGAGAACAGAACTTTTCAAACAAC
  TGAGTTTTCTTGAGACAGTTAACCTTTTAAGAAGGGAGGAAGATGATCCGGTCCCTGCCCACCATGACCG
  TCCTCATCCCACTGGTCTCGCTGGCAGGCCTGCTCTACTCCGCCTCTGTGGAGGAAGGCTTCCCAGAGGG
  CTGCACCAGCGCCAGCAGCCTGTGCTTCTACAGCTTGCTCTTGCCGGTCACCGTGCCTGTGTACGTGTTC
  TTCCATCTGTGGACATGGATGGGCCTGAAGCTCTTCAGACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200384 representing BC025114
  Red=Cloning site Green=Tags(s)
  
  MRSRDKGKIRQERTRMRTELFKQLSFLETVNLLRREEDDPVPAHHDRPHPTGLAGRPALLRLCGGRLPRG
  LHQRQQPVLLQLALAGHRACVRVLPSVDMDGPEALQT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC025114
• ORF Size: 323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC025114
• RefSeq Size: 664
• RefSeq ORF: 323
• Gene Summary: This gene encodes a homolog of a human protein that functions in glycosylphosphatidylinositol biosynthesis. The human protein is expressed from an unusual locus that encodes two distinct proteins in upstream and downstream CDSes; however, in mouse these two proteins are expressed from distinct loci. The product of this locus is highly similar to the protein expressed from the human downstream CDS. A separate mouse locus on chromosome 6 is orthologous to the human locus and encodes a protein similar to the human protein expressed from the upstream CDS. [provided by RefSeq, Jul 2008]