Mt1 (BC027262) Mouse Tagged ORF Clone

CAT#: MG200758

Mt1 (GFP-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mt1
• Synonyms: MT-I
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200758 representing BC027262
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCAACTGCTCCTGCTCCACCGGCGGCTCCTGCACTTGCACCAGCTCCTGCGCCTGCAAGAACT
  GCAAGTGCACCTCCTGCAAGAAGAGTGAGTTGGGACACCTTGGGTGGCGGCTAAGGCTAGGGGCGGGGAA
  CTCCTACAAAACTGGCTCTGAGAAATGTCCTTTGCTTCCCGGAGGCCATTGTATTGTCTCGGGGACAGAA
  CTATACAGAGAACTATTTAAAAAAACCGAGGTCTTCTCTGTTGGGGACAGGAAGCAGAGGTCTTCAGCCA
  GGCTGACCTCTTCCTCCTCCTTTCTAGGCTGCTGCTCCTGCTGTCCCGTGGGCTGCTCCAAATGTGCCCA
  GGGCTGTGTCTGCAAAGGCGCCGCGGACAAGTGCACGTGCTGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200758 representing BC027262
  Red=Cloning site Green=Tags(s)
  
  MDPNCSCSTGGSCTCTSSCACKNCKCTSCKKSELGHLGWRLRLGAGNSYKTGSEKCPLLPGGHCIVSGTE
  LYRELFKKTEVFSVGDRKQRSSARLTSSSSFLGCCSCCPVGCSKCAQGCVCKGAADKCTCCA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027262
• ORF Size: 398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC027262, AAH27262
• RefSeq Size: 673
• RefSeq ORF: 398
• Locus ID: 17748
• Gene Summary: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. [UniProtKB/Swiss-Prot Function]