Mt1 (BC027262) Mouse Tagged ORF Clone

CAT#: MR200758

Mt1 (Myc-DDK-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mt1
• Synonyms: MT-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200758 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCAACTGCTCCTGCTCCACCGGCGGCTCCTGCACTTGCACCAGCTCCTGCGCCTGCAAGAACT
  GCAAGTGCACCTCCTGCAAGAAGAGTGAGTTGGGACACCTTGGGTGGCGGCTAAGGCTAGGGGCGGGGAA
  CTCCTACAAAACTGGCTCTGAGAAATGTCCTTTGCTTCCCGGAGGCCATTGTATTGTCTCGGGGACAGAA
  CTATACAGAGAACTATTTAAAAAAACCGAGGTCTTCTCTGTTGGGGACAGGAAGCAGAGGTCTTCAGCCA
  GGCTGACCTCTTCCTCCTCCTTTCTAGGCTGCTGCTCCTGCTGTCCCGTGGGCTGCTCCAAATGTGCCCA
  GGGCTGTGTCTGCAAAGGCGCCGCGGACAAGTGCACGTGCTGTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200758 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPNCSCSTGGSCTCTSSCACKNCKCTSCKKSELGHLGWRLRLGAGNSYKTGSEKCPLLPGGHCIVSGTE
  LYRELFKKTEVFSVGDRKQRSSARLTSSSSFLGCCSCCPVGCSKCAQGCVCKGAADKCTCCA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027262
• ORF Size: 396 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027262, AAH27262
• RefSeq Size: 673 bp
• RefSeq ORF: 398 bp
• Locus ID: 17748
• MW: 13.8 kDa
• Gene Summary: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. [UniProtKB/Swiss-Prot Function]