Il1rn (BC042532) Mouse Tagged ORF Clone

CAT#: MG201227

Il1rn (GFP-tagged) - Mouse interleukin 1 receptor antagonist (cDNA clone MGC:35609 IMAGE:4486098)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il1rn
• Synonyms: IL-1ra
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201227 representing BC042532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAGAGGCAGCCTGCCGCCCTTCTGGGAAAAGACCCTGCAAGATGCAAGCCTTCAGAATCTGGG
  ATACTAACCAGAAGACCTTTTACCTGAGAAACAACCAGCTCATTGCTGGGTACTTACAAGGACCAAATAT
  CAAACTAGAAGAAAAGTTAGACATGGTGCCTATTGACCTTCATAGTGTGTTCTTGGGCATCCACGGGGGC
  AAGCTGTGCCTGTCTTGTGCCAAGTCTGGAGATGATATCAAGCTCCAGCTGGAGGAAGTTAACATCACTG
  ATCTGAGCAAGAACAAAGAAGAAGACAAGCGCTTTACCTTCATCCGCTCTGAGAAAGGCCCCACCACCAG
  CTTTGAGTCAGCTGCCTGTCCAGGATGGTTCCTCTGCACAACACTAGAGGCTGACCGTCCTGTGAGCCTC
  ACCAACACACCGGAAGAGCCCCTTATAGTCACGAAGTTCTACTTCCAGGAAGACCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201227 representing BC042532
  Red=Cloning site Green=Tags(s)
  
  MASEAACRPSGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNIKLEEKLDMVPIDLHSVFLGIHGG
  KLCLSCAKSGDDIKLQLEEVNITDLSKNKEEDKRFTFIRSEKGPTTSFESAACPGWFLCTTLEADRPVSL
  TNTPEEPLIVTKFYFQEDQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC042532
• ORF Size: 479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC042532, AAH42532
• RefSeq Size: 2470
• RefSeq ORF: 479
• Locus ID: 16181
• Gene Summary: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. [UniProtKB/Swiss-Prot Function]